CD246 / Anaplastic lymphoma kinase antibody

Principal name

CD246 / Anaplastic lymphoma kinase antibody

Alternative names for CD246 / Anaplastic lymphoma kinase antibody

ALK tyrosine kinase receptor

SwissProt ID

P97793 (Mouse), Q9UM73 (Human)

Gene ID

238 (ALK), 11682 (Alk)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD246 / Anaplastic lymphoma kinase antibody

ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.

General readings

1. Pittaluga S, et al. American Journal of Pathology. 151(2):343-351 (1997).
2. Pulford K, et al. Blood. 89(4): 1394-1404 (1997).
3. Downing J R, et al. Bloob. 85(12): 3416-3422(1995).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTIB2 (formerly B2)  
Immunofluorescent staining of HeLa cells using anti-ALK mouse monoclonal antibody (TA800724) Mouse IgG1 OTIB2 Purified Hu ICC/IF, WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)  
Immunohistochemical staining of paraffin-embedded Human non-small cell lung cancer sample with ALK translocation detected by FISH  using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b OTI1A4 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

AP31403PU-N Alk antibody staining of Rat Brain lysate at 0.03 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD246 / Anaplastic lymphoma kinase antibody

  Rabbit polyclonal anti-ALK antibody  
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ALK antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

CD246 / Anaplastic lymphoma kinase antibody

  Rabbit polyclonal anti-ALK antibody  
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ALK antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

CD246 / Anaplastic lymphoma kinase antibody

  Rabbit polyclonal ALK (Ab-1586) antibody  
Western blot analysis of extracts from HepG2 cells, using ALK (Ab-1586) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CD246 / Anaplastic lymphoma kinase antibody

  Anti-ALK Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using ALK antibody at dilution 1/25, on the right is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ALK antibody at dilution 1/25. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

CD246 / Anaplastic lymphoma kinase antibody

  Rabbit Polyclonal ALK Antibody  
Western blot analysis of ALK expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CD246 / Anaplastic lymphoma kinase antibody

  Rabbit Polyclonal ALK (Tyr1507) Antibody (Phospho-specific)  
Western blot analysis of ALK phosphorylation expression in anisomycin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI1F1 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI1B1 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI1H2 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI6G8 (formerly 6G8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI6G8 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI5C8 (formerly 5C8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG2b OTI5C8 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI5E6 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI5D1 (formerly 5D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI5D1 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1D4 (formerly 1D4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG1 OTI1D4 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1H7 (formerly 1H7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG2b OTI1H7 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI7E11 (formerly 7E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Mouse IgG2b OTI7E11 Purified Hu WB
0.1 ml / €350.00
  OriGene Technologies, Inc.

TrueMAB

CD246 / Anaplastic lymphoma kinase antibody

  ALK mouse monoclonal antibody, clone OTI1E12 (formerly 1E12)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ALK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800730) Mouse IgG1 OTI1E12 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

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