CD247 / CD3Z antibody

Principal name

CD247 / CD3Z antibody

Alternative names for CD247 / CD3Z antibody

T3Z, TCRZ, CD3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain

SwissProt ID

P20963 (Human), P24161 (Mouse), Q9XSJ9 (Pig)

Gene ID

919 (CD247), 12503 (Cd247)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep)

Available hosts

Rabbit, Mouse, Hamster, Goat

Available applications

Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Microcytotoxicity (MC), Functional assay (FN)

Background of CD247 / CD3Z antibody

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζη). The ζ and η chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the ζ chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the ζ chain ITAMs has been solved.

General readings

Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46Rieux-Laucat F., et al. (2006) N. Engl. J. Med. 354:1913-1921

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD247 / CD3Z antibody

Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-CD247 antibody Cat.-No AM06414SU-N Mouse IgG1 4B10 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CD247 / CD3Z (1-165) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4A12-F6 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-CD247 mouse mAb Mouse IgG1 4B10 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

CD247 / CD3Z antibody

Human tonsil stained with anti-CD3 zeta antibody Rabbit Purified Hu, Ms, Rt IP, P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

Human tonsil stained with anti-CD3 zeta antibody Rabbit Purified Hu, Ms, Rt IP, P
1 ml / €440.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

Human tonsil stained with anti-CD3 zeta antibody Rabbit Purified Hu, Ms, Rt IP, P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD247 / CD3Z (C-term) antibody

Western blot analysis of CD3Z (arrow) using rabbit polyclonal CD3Z Antibody (C-term) (Cat.#1413b).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD3Z gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD247 / CD3Z antibody

Predicted band size: 16 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD247 / CD3Z antibody

Western blot analysis of extracts of Jurkat cell line, using CD247 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD247 / CD3Z antibody

Jurkat (1) and Raiji (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-CD247 (TCR zeta, CD3 zeta) antibody.  Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 6B10.2 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD247 / CD3Z antibody

TA341382 (0.03µg/ml) staining of Human Tonsil (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD247 / CD3Z antibody

Western blot analysis of CD3 ? expression in Jurkat cell lysa Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD247 / CD3Z antibody

Flow Cytometric analysis CD3 expressed in Human peripheral blood cells. Mouse IgG2a 4D10A6 Lyophilized Hu E, F
0.1 ml / €280.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

CD247 / CD3Z Hamster IgG H146-968 Purified Hu, Ms F, ICC/IF
0.3 mg / €480.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

CD247 / CD3Z Hamster IgG H146-968 Purified Hu, Ms F, ICC/IF
0.1 mg / €260.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

CD247 / CD3Z Hamster IgG H146-968 FITC Hu, Ms F, ICC/IF
0.3 mg / €480.00
  Acris Antibodies GmbH

CD247 / CD3Z antibody

Western Blot Analysis of AM12004PU-N CD3z antibody: Lysate from Mouse Spleen and Thymus (Thymus shown) cells probed with AM12004PU-N at 1.0 µg/ml. AP conjugated Goat Anti-Hamster IgG used as a secondary antibody. Hamster IgG H146-968 Purified Hu, Ms E, F, ICC/IF, WB
0.25 mg / €370.00
  Acris Antibodies GmbH

CD247 / CD3Z Tyr153 antibody

Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively. Mouse IgG1 EM-17 Purified Hu, Ms F, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD247 / CD3Z pTyr111 antibody

CD247 / CD3Z Mouse IgG1 EM-55 Purified Hu, Ms F, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD247 / CD3Z pTyr142 antibody

CD247 / CD3Z Mouse IgG1 EM-54 Purified Hu, Ms F, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

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