CD249 / Glutamyl aminopeptidase antibody

Principal name

CD249 / Glutamyl aminopeptidase antibody

Alternative names for CD249 / Glutamyl aminopeptidase antibody

EAP, Aminopeptidase A, ENPEP, Differentiation antigen gp160

SwissProt ID

P16406 (Mouse), P50123 (Rat), Q07075 (Human), Q32LQ0 (Bovin), Q95334 (Pig)

Gene ID

13809 (Enpep), 2028 (ENPEP)

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine), Por (Porcine), Rt (Rat), Bov (Bovine), Mky (Monkey)

Available hosts

Rat, Goat, Rabbit, Mouse

Available applications

Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD249 / Glutamyl aminopeptidase antibody

Aminopeptidase A, also designated APA, gp160 human kidney differentiation antigen, glutamyl aminopeptidase, or enpep, is a differentiation-related kidney glycoprotein. As a cell surface, zinc-dependent metalloprotease, Aminopepti-dase A specifically cleaves amino-terminal acidic residues from peptide substrates such as Angiotensin II. APA is expressed on the surface of epithelial cells of the glomerulus and proximal tubule cells of the human nephron, where it may mediate the constitutive trafficking of Glut4-containing vesicles. These Glut4-containing vesicles are tissue-specific secretory-like microsomal structures that mediate insulin-dependent translocation of GLUT4 to the cell surface in fat and muscle cells. Mutations in the gp160/APA gene, including loss of protein expression or enzymatic activity, occur in 20% of primary clear cell renal carcinomas.

General readings

Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010).
Rose, J. Phd, et al. Mol. Med. (2010) In press.
Perez, I., et al. Head Neck 31(10):1335-1340(2009).
Sevalle, J., et al. J. Neurochem. 109(1):248-256(2009).
Teranishi, J., et al. Prostate 68(15):1666-1673(2008).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503962) Mouse IgG2a OTI3E7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503963) Mouse IgG1 OTI1C4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504037) Mouse IgG2a OTI1D4 Purified Hu, Mky, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504054) Mouse IgG2b OTI3G1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504071) Mouse IgG2b OTI1E3 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504251) Mouse IgG2b OTI4G8 Purified Hu, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

TA303036 staining (1µg/ml) of human testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD249 / Glutamyl aminopeptidase antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENPEP (RC210521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENPEP. Mouse IgG2a OTI1D1 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Anti-ENPEP mouse monoclonal antibody (TA503915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ENPEP(RC210521). Mouse IgG2b OTI1D6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD249 / Glutamyl aminopeptidase antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ENPEP rabbit polyclonal antibody. (TA590717) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD249 / Glutamyl aminopeptidase antibody

CD249 / Glutamyl aminopeptidase Rat IgG1 FG35.4 Biotin Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase antibody

CD249 / Glutamyl aminopeptidase Rat IgG1 FG35.4 FITC Ms F
0.5 mg / €400.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase antibody

CD249 / Glutamyl aminopeptidase Rat IgG1 FG35.4 Purified Ms F, IP
0.5 mg / €290.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase antibody

Immunofluorescent Staining: Cells from BALB/c fetal liver were cultured with IL-7-producing stromal cells for 3 days at 37°C, stained with Rat anti-Mouse BP1/6C3-PE, and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.2 µg/10e6 cells. Rat IgG1 FG35.4 PE Ms F
0.2 mg / €360.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase antibody

Immunofluorescent Staining: Cells from BALB/c fetal liver were cultured with IL-7-producing stromal cells for 3 days at 37°C, stained with Rat anti-Mouse BP1/6C3-PE, and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.2 µg/10e6 cells. Rat IgG1 FG35.4 PE Ms F
0.1 mg / €300.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase (805-818) antibody

Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Bt, Can, Elephant, Eq, Hu, Mky, Ms, Por, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase (689-032) antibody

Human Brain, cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Por, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase (C-term) antibody

Formalin fixed and paraffin embedded human kidney tissue reacted with CD249 / Glutamyl aminopeptidase Antibody (C-term) Cat.-No AP51433PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD249 / Glutamyl aminopeptidase antibody

ENPEP polyclonal antibody ( Cat # PAB6806 ) staining ( 1 µg/ml ) of human testis lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD249 / Glutamyl aminopeptidase antibody

ELISA: Aminopeptidase A / APA Antibody [43180002] Rabbit Hu E, WB
50 µg / €480.00
  Novus Biologicals Inc.

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