CD25 / IL2RA antibody

Principal name

CD25 / IL2RA antibody

Alternative names for CD25 / IL2RA antibody

Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen

SwissProt ID

O02733 (Pig), O62802 (Canfa), P01589 (Human), P01590 (Mouse), P12342 (Bovin), P26897 (Rat), P26898 (Sheep), P41690 (Felca)

Gene ID

25704 (Il2ra), 3559 (IL2RA), 443435, 281861, 16184, 396814

Available reactivities

Rt (Rat), Hu (Human), Sh (Sheep), Bov (Bovine), Mky (Monkey), Ms (Mouse), Por (Porcine)

Available hosts

Mouse, Rat, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Functional assay (FN), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), ELISA (capture) (E(capture))

Background of CD25 / IL2RA antibody

The biological activities of IL-2 are mediated by its binding to a multi-molecular cellular receptor complex. For several years the receptor was thought to consist of two glycoprotein chains, an alpha chain (IL-2 R.) and a beta chain (IL-2 R.) , which acted together to form a high affinity receptor that transduced the IL-2 signal. IL-2 R. (also known as Tac antigen and as CD25) is a 55 kDa transmembrane glycoprotein composed of 351 amino acids with only 13 located on the cytoplasmic side of the membrane . The second chain of the complex was cloned in 1989 , and is a transmembrane glycoprotein of 575 amino acids (75 kDa), 286 of which are located cytoplasmically and clearly participate in signal transduction . Eventually it was discovered that a third chain, IL-2 R.,was necessary for high affinity binding, ligand internalization and signalling. Constitutively expressed on many lymphoid cells, it had been overlooked partly because it has no affinity for IL-2 except when IL-2 R. is present . When cloned, the gene was found to code for a 64 kDa transmembrane protein of 347 amino acids, 84 of which are cytoplasmic . Both IL-2 R. and IL-2 R. are members of the hematopoietin receptor superfamily, whereas IL-2 R. is related only to the IL-15 R .chain .

A model of the IL-2 receptor complex (3, 9, 16 - 21) would describe the high affinity receptor as an ... trimer, in which all three chains are in contact with the ligand. Alone, IL-2 R. binds IL-2 with low affinity, but is unable to transduce a signal. The .. combination will bind IL-2 with intermediate affinity, but still will not transduce a signal. A .. complex has intermediate affinity and is capable of signalling if the IL-2 concentration is relatively high. Regardless of many subtleties that determine the affinity of the ligand for the extracellular portions of the receptor components , signalling will ensue if the .and .cytoplasmic domains are brought into close proximity .

A soluble form of IL-2 R. appears in serum, concomitant with its increased expression on cells . There are reports of a soluble form of IL-2 R. as well . The function of the soluble IL-2 R. is unclear, since it would be expected to be a poor inhibitor of IL-2 because of its low binding affinity. In any case, increased levels of the soluble IL-2 R. in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Results of a number of studies suggest a correlation of levels of IL-2 sR. in serum with the onset of rejection episodes in allograft recipients , with activity of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosis (SLE) and with the course of some leukemias and lymphomas .

General readings

1. Lai KN, Leung JC, Lai FM: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro. Pathology. 1991 Jul;23(3):224-8.
2. Scheibenbogen C, Keilholz U, Richter M, Andreesen R, Hunstein W: The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75). Res Immunol. 1992 Jan;143(1):33-7.
3. Morris JC, Waldmann TA: Advances in interleukin 2 receptor targeted treatment. Ann Rheum Dis. 2000 Nov;59 Suppl 1:i109-14.
4. Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P: CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol. 2004 Oct;28(10):1319-25.
5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
6. Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD25 / IL2RA antibody

Western Blot analysis of MOLT-4 cell lysate with CD25 antibody. Rabbit IgG SP176 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD25 / IL2RA (C-term) antibody


Human tonsil stained with anti-CD25 antibody
Rabbit IgG SP176 Purified Hu P, WB
0.5 ml / €530.00
  Acris Antibodies GmbH

CD25 / IL2RA (C-term) antibody


Human tonsil stained with anti-CD25 antibody
Rabbit IgG SP176 Purified Hu P, WB
1 ml / €760.00
  Acris Antibodies GmbH

CD25 / IL2RA (C-term) antibody


Human tonsil stained with anti-CD25 antibody
Rabbit IgG SP176 Purified Hu P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

  Rabbit polyclonal IL-2Ra/CD25 (Ser268) antibody(Phospho-specific)  
Western blot analysis of extracts from LOVO cells, using IL-2Ra/CD25 (Phospho-Ser268) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD25 / IL2RA antibody

Staining of PHA stimulated human PBMC with Anti-Human CD25 PE.  Appropriate isotype controls were used (open histogram).  Total viable cells were used for analysis. Mouse BC96 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD25 / IL2RA antibody

Predicted band size: 31 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD25 / IL2RA antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using IL2RA antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD25 / IL2RA antibody

PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone BC96) (solid line) or purified mouse IgG1, kappa; isotype control (dotted line). Mouse BC96 Purified Hu F
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD25 / IL2RA antibody

ELISA: IL2 Receptor alpha Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 NR-72 Purified Hu F, FN
0.2 mg / €220.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 IL-A111 FITC Bov, Sh F
0.1 mg / €530.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 IL-A111 PE Bov, Sh F
100 Tests / €570.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG2a Azide Free Hu C, E, FN, WB
0.5 mg / €200.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 1TYV APC Hu F
100 Tests / €390.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 1TYV Biotin Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 1TYV FITC Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 1TYV Purified Hu F
0.1 mg / €250.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

Figure 1. Peripheral blood lymphocytes stained with PE labeled anti-CD25 antibody (AM05184RP-N). Mouse IgG1 1TYV PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD25 / IL2RA antibody

CD25 / IL2RA Mouse IgG1 BC96 PE-Cy5 Hu, Mky F
100 TESTS/2.0ml / €430.00
  Acris Antibodies GmbH

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