CD254 / RANKL antibody

Principal name

CD254 / RANKL antibody

Alternative names for CD254 / RANKL antibody

OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, Osteoprotegerin ligand, Osteoclast differentiation factor

SwissProt ID

O14788 (Human), O35235 (Mouse), Q9ESE2 (Rat)

Gene ID

8600 (TNFSF11), 21943 (Tnfsf11), 117516

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Functional assay (FN), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Neutralisation (NEUT)

Background of CD254 / RANKL antibody

TRANCE, also called RANKL (RANK ligand), OPGL (Osteoprotegrin ligand), and ODF (Osteoclast differentiation factor), is a type II transmembrane signaling protein of the TNF superfamily. The extracellular domain contains two potential N-linked glycosylation sites. Mouse TRANCE cDNA encodes a 316 amino acid protein with a calculated molecular mass of approximately 28 kDa. As a result of glycosylation, recombinant mouse TRANCE migrates as an approximately 36 kDa protein in SDS-PAGE. Human and mouse TRANCE share approximately 85% amino acid sequence identity. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. It is a key regulator of the immune system and of bone development and homeostasis. Multi-functions of TRANCE include induction of activation of c-jun N-terminal kinase in T cells, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis and lymph node organogenesis. The cell surface signaling receptor of TRANCE (RANKL) is RANK which undergoes receptor clustering during signal transduction. TRANCE is highly expressed in thymus and lymph nodes but not in nonlymphoid tissues.1 It is abundantly expressed in T cells but not in B cells. TRANCE activates mature dendritic cells, inducing cytokine production, suggesting that it is a factor in the T cell dentritic cell interaction during an immune response. TRANCE expression is found on osteoblasts and regulates osteoclast differentiation function.

General readings

1. Grimaud E (2003); Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis.
American Journal of Pathology; 163(5):2021-31

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD254 / RANKL antibody

Western blot analysis of extracts of various cell lines, using TNFSF11 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Western blot analysis of sRANK-L in rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. RANKL (CD254) antibody. TA308970 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Sample (30 ug of whole cell lysate). A: Molt-4 . 10% SDS PAGE. TA308971 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Western blot analysis of extracts from HuvEc cells, using TNF11 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Anti-mouse RANKL antibody in WB shows detection of recombinant mouse RANKL raised in E.coli. Recombinant truncated protein (0.1 µg, 19.9 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was incubated with Anti-Mouse RANKL antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000. Rabbit IgG Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

CD254 / RANKL Rat IK22/5 Purified Ms F
50 µg / €200.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml.  Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml.  Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

To detect msRANKL by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant msRANKL. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

To detect msRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine sRANKL (TA328546) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant msRANKL. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Western Blot: RANKL Antibody [TA336347] - analysis of CD254. A. A CD254 recombinant protein fragment. Note: this is not a full-length protein. B. LNCaP lysate using CD254 antibody at 0.5 ug/ml. Rabbit Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Western Blot: RANKL Antibody (12A668) [TA336377] - Western blot analysis of human lymph node lysate (35ug per lane, RIPA buffer) using RANKL antibody (TA336377) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_00369 Mouse IgG1 12A668 Purified Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Western Blot: RANKL Antibody (12A380) [TA336475] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml. Mouse IgG1 12A380 Purified Hu, Ms C, F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

Western blot analysis on HuvEc cell lysate using TNFSF11 Antibody Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL (287-304) antibody

CD254 / RANKL Rabbit IgG Purified Hu E
0.1 mg / €540.00
  Acris Antibodies GmbH

CD254 / RANKL (287-304) antibody

CD254 / RANKL Rabbit IgG Purified Hu E
10 µg / €190.00
  Acris Antibodies GmbH

CD254 / RANKL (287-304) antibody

CD254 / RANKL Rabbit Serum Hu E
0.1 ml / €480.00
  Acris Antibodies GmbH

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