CD254 / RANKL antibody

Principal name

CD254 / RANKL antibody

Alternative names for CD254 / RANKL antibody

OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, Osteoprotegerin ligand, Osteoclast differentiation factor

SwissProt ID

O14788 (Human), O35235 (Mouse), Q9ESE2 (Rat)

Gene ID

8600 (TNFSF11), 21943 (Tnfsf11), 117516

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of CD254 / RANKL antibody

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kise AKT/PKB through a sigling complex involving SRC kise and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregncy. Two altertively spliced transcript variants have been found. [provided by RefSeq]

General readings

1. Grimaud E (2003); Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis.
American Journal of Pathology; 163(5):2021-31

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD254 / RANKL antibody

  Rabbit anti-TNFSF11 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using TNFSF11 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit Polyclonal sRANK Ligand Antibody  
Western blot analysis of sRANK-L in rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit Polyclonal antibody to RANKL (tumor necrosis factor (ligand) superfamily, member 11)  
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. RANKL (CD254) antibody. TA308970 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit polyclonal antibody to RANKL (CD254) (tumor necrosis factor (ligand) superfamily, member 11)  
Sample (30 ug of whole cell lysate). A: Molt-4 . 10% SDS PAGE. TA308971 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit polyclonal anti-TNF11 antibody  
Western blot analysis of extracts from HuvEc cells, using TNF11 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit polyclonal anti-RANK-L antibody  
Anti-mouse RANKL antibody in WB shows detection of recombinant mouse RANKL raised in E.coli. Recombinant truncated protein (0.1 µg, 19.9 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was incubated with Anti-Mouse RANKL antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000. Rabbit IgG Purified Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rat Anti-Mouse CD254 (RANK Ligand) Purified (50 ug)  
CD254 / RANKL Rat IK22/5 Purified Ms F
50 µg / €200.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Biotinylated Anti-Human sRANK Ligand Rabbit Polyclonal Antibody  
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Biotinylated Anti-Human sRANK Ligand Goat Polyclonal Antibody  
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Anti-Human sRANK Ligand Goat Polyclonal Antibody  
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml.  Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Anti-Human sRANK Ligand Rabbit Polyclonal Antibody  
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml.  Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Biotinylated Anti-Murine sRANK Ligand Rabbit Polyclonal Antibody  
To detect msRANKL by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant msRANKL. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Anti-Murine sRANK Ligand Rabbit Polyclonal Antibody  
To detect msRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine sRANKL (TA328546) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant msRANKL. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit Polyclonal TRANCE/TNFSF11/RANK L Antibody  
Western Blot: RANKL Antibody [TA336347] - analysis of CD254. A. A CD254 recombinant protein fragment. Note: this is not a full-length protein. B. LNCaP lysate using CD254 antibody at 0.5 ug/ml. Rabbit Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody (12A668)  
Western Blot: RANKL Antibody (12A668) [TA336377] - Western blot analysis of human lymph node lysate (35ug per lane, RIPA buffer) using RANKL antibody (TA336377) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_00369 Mouse IgG1 12A668 Purified Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody (12A380)  
Western Blot: RANKL Antibody (12A380) [TA336475] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml. Mouse IgG1 12A380 Purified Hu, Ms C, F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rabbit Polyclonal Anti-TNFSF11 Antibody  
Western blot analysis on HuvEc cell lysate using TNFSF11 Antibody Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD254 / RANKL antibody

  Rat Monoclonal anti-TRANCE Antibody  
Mouse TRANCE transfected cells stained with purified IK22-5, followed by biotinylated anti-rat IgG and Sav-PE Rat IK22/5 Purified Ms F, IP, WB
0.5 mg / €379.00
  OriGene Technologies, Inc.

CD254 / RANKL (287-304) antibody

CD254 / RANKL Rabbit IgG Purified Hu E
0.1 mg / €540.00
  Acris Antibodies GmbH

CD254 / RANKL (287-304) antibody

CD254 / RANKL Rabbit IgG Purified Hu E
10 µg / €190.00
  Acris Antibodies GmbH

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