CD257 / BAFF antibody

Principal name

CD257 / BAFF antibody

Alternative names for CD257 / BAFF antibody

TNFSF13B, BLYS, TALL1, TNFSF20, ZTNF4, Tumor necrosis factor ligand superfamily member 13B, TNF- and APOL-related leukocyte expressed ligand 1, B lymphocyte stimulator, B cell-activating factor, BAFF, Dendritic cell-derived TNF-like molecule

SwissProt ID

Q9WU72 (Mouse), Q9Y275 (Human)

Gene ID

10673 (TNFSF13B), 24099 (Tnfsf13b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine)

Available hosts

Mouse, Goat, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of CD257 / BAFF antibody

The T7-241 antibody recognizes human BAFF also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog that activates apoptosis, NF- kappaB, and JNK (THANK). BAFF is a type II transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells and is upregulated by IFN- gamma and downregulated by PMA/ionomycin treatment. The T7-241 antibody has been shown to be useful for flow cytometry and ELISA. BAFF stimulates B and T cell immunity and regulates humoral immunity by binding TACI and BCMA receptors; BAFF prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3). The T7-241 antibody has been shown to be useful for flow cytometry and ELISA.

General readings

1. Perchonock, C.E. The related adaptors, adaptor in lymphocytes of unknown function x and rlk/itk-binding protein, have nonredundant functions in lymphocytes. The Journal of Immunology.;179(3):1768-75. 2007.
2. Sakurai D, TACI regulates IgA production by APRIL in collaboration with HSPG Blood. ;109(7):2961-7. 2007.
3. Debnath, I. Analysis of the regulatory role of BAFF in controlling the expression of CD21 and CD23. Molecular Immunology.;44(9):2388-99. 2007.
4. Tangye, S.G. BAFF, APRIL and human B cell disorders. Seminars in Immunology.;18(5):305-17. 2006.
5. Enzler T Alternative and Classical NF-kappaB Signaling Retain Autoreactive B Cells in the Splenic Marginal Zone and Result in Lupus-like Disease Immunity. ;25(3):403-15. 2006.
6. Bischof D Selective activation of TACI by syndecan-2. Blood.;107(8):3235-42. 2006.
Aiba Y BANK negatively regulates Akt activation and subsequent B cell responses. Immunity.;24(3):259-68. 2006.
7. Ohmae T Helicobacter pylori Activates NF-{kappa}B via the Alternative Pathway in B Lymphocytes Journal of Immunology;175(11):7162-9. 2005.
8. Yang M B Cell Maturation Antigen, the Receptor for a Proliferation-Inducing Ligand and B Cell-Activating Factor of the TNF Family, Induces Antigen Presentation in B Cells Journal of Immunology;175(5):2814-24. 2005.
9. Hidenori Hase BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex Blood, VOl: 103 (6) pp. 2257-2265. 2004.
10. Jerome Moreaux BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone Blood, Vol: 103 (8) pp. 31483157, 2004.
11. Estefania Claudio BAFF-induced NEMO-independent processing of NF-kappa B2 in maturing B cells Nature Immunology 3 pp 958-965, 2002.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD257 / BAFF (C-term) antibody

Western blot analysis of BAFF in human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with BAFF antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD257 / BAFF antibody

To detect hBAFF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBAFF. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD257 / BAFF antibody

To detect hBAFF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BAFF (TA328258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBAFF. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD257 / BAFF antibody

Western blot analysis of extracts of various cell lines, using TNFSF13B antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD257 / BAFF antibody

Western Blot: TNFSF13B Antibody [TA336332] - Analysis of TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). I goat anti-rabbit Ig HRP secon Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD257 / BAFF antibody

WB Suggested Anti-TNFSF13B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Eq, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD257 / BAFF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFSF13B (RC204672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFSF13B.(1:500) Mouse IgG2a OTI7F12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD257 / BAFF (134-285) antibody

Western blot analysis: Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 H4-C7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD257 / BAFF (134-285) antibody

Western blot analysis: Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 H4-C7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

CD257 / BAFF Mouse IgG1 2B Purified Hu E
0.5 mg / €430.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG1 T7-241 Aff - Purified Hu E, F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

Immunohistochemical detection of BAFF/BLyS on paraffin embedded sectiof BAFF/BLyS transfected cells (left) and mock transfected cells (right) with AM26518AF-N. Mouse IgG1 1D6 Azide Free Hu F, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

Flow cytometric analysis of CD257/BAFF/BLyS expression on BAFF transfected cells (right) and parental cells (left). Open histogram indicates the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26518FC-N to the cells. Mouse IgG1 1D6 FITC Hu F
0.1 ml / €340.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

Immunocytochemical staining of HL60 promyelocytin leukaemic cells with Rabbit anti CD257 (AP05831PU-N) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CD257 / BAFF (C-term) antibody

AP08477PU-N CD257/BAFF antibody staining of Formalin-Fixed Paraffin-Embedded Human Tonsil at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD257 / BAFF Control Peptide

CD257 / BAFF
50 µg / €160.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

CD257 / BAFF Mouse IgG1 Azide Free Hu E, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

CD257 / BAFF Goat Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

CD257 / BAFF Goat Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

CD257 / BAFF antibody

CD257 / BAFF Goat Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

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