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CD26 / DPP4 antibody

Principal name

CD26 / DPP4 antibody

Alternative names for CD26 / DPP4 antibody

ADCP2, Dipeptidyl peptidase 4, DPP IV, Dipeptidyl peptidase IV, ADABP, TP103, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2

SwissProt ID

P14740 (Rat), P22411 (Pig), P27487 (Human), P28843 (Mouse), P81425 (Bovin), Q9N2I7 (Felca)

Gene ID

1803 (DPP4), 13482 (Dpp4), 281122, 25253

Ncbi ID

NP_001926.2, NP_001153015.1, 9606, NP_776464.1, 10116, NP_001926, NP_036921.1, NM_001935, NM_001159543, NM_010074, NM_012789

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Sh (Sheep), Mky (Monkey)

Available hosts

Mouse, Rat, Goat, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of CD26 / DPP4 antibody

Kit Component:: - KN304781G1, Dpp4 gRNA vector 1 in pCas-Guide vector
- KN304781G2, Dpp4 gRNA vector 2 in pCas-Guide vector
- KN304781D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

1. Kameoka J, Tanaka T, Nojima Y, Schlossman SF, Morimoto C (July 1993). "Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science 261 (5120): 466–9.
2． Darmoul, D., Lacasa, M., Chantret, I., Swallow, D. M., Trugnan, G.Isolation of a cDNA probe for the human intestinal dipeptidylpeptidase IV and assignment of the gene locus DPP4 to chromosome 2.Ann. Hum. Genet. 54: 191-197, 1990

SM1085P staining of human peripheral blood lymphocytes.

SM1085P

SM1085PS staining of human peripheral blood lymphocytes

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD26:RPE

Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD26 (SM2264P).

SM2264P

Detection limit for recombinant GST tagged DPP4 is approximately 1ng/ml as a capture antibody.

H00001803-M03

DPP4 polyclonal antibody ( Cat # PAB7296 ) ( 0.3 µg/ml ) staining of rat lung lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7296

Western Blot analysis of DPP4 expression in transfected 293T cell line (H00001803-T01) by DPP4 MaxPab polyclonal antibody.Lane 1: DPP4 transfected lysate(88.30 KDa).Lane 2: Non-transfected lysate.

H00001803-D01P

Recombinant human CD26, His-tagged

CD26: Optical density was measured at 405 nm after incubating enzyme solution with 1 mM of p-nitroanilid as a substrate.

CD26 (DPP4): 10% SDS-PAGE (2 ug)

Western blot analysis of DPP4 expression using Jurkat cell lysate.

PAB8687

Flow Cytometry using human peripheral blood cells ( 1 µg/10⁶ cells ).FACSCalibur ( Becton Dickinson, San Jose, CA ) flow cytometer, using Cell Quest acquisition software and PAINT-A-GATE. PRO, analysis software.

MAB4975

Immunohistochemical detection of human CD26 on paraffin embedded section of human Thyroid carcinoma.

AM26442SU-L

Flow cytometry

AM26442SU-L

Representative Standard Curve

CEK1087

Representative Standard Curve

CEK1413

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-DPP4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500733, Dilution 1:50)

TA500733

Figure from citation: Immunohistochemistry of DPP4 protein level by using anti-DPP4 antibody in lungs harvested from BALB/c mice at day 3. Dilution: 1:800 View Citation

TA500733

Anti-DPP4 mouse monoclonal antibody (TA500733) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4(RC209466).

TA500733

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP4 (RC209466, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP4.

TA500733

Figure from citation: Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells. View Citation

TA500733

Flow cytometric analysis of Hela cells, using anti-DPP4 antibody(TA500733),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

TA500733

TP309466

TA303245 (0.3µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303245

Staining of normal human peripheral blood cells with Anti-Human CD26 FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

TA320322