CD266 / TweakR antibody

Principal name

CD266 / TweakR antibody

Alternative names for CD266 / TweakR antibody

FGF-inducible 14, FN14, TNFRSF12A, Tweak receptor, Tumor necrosis factor receptor superfamily member 12A, Fibroblast growth factor-inducible immediate-early response protein 14

SwissProt ID

Q9CR75 (Mouse), Q9NP84 (Human)

Gene ID

51330 (TNFRSF12A), 27279 (Tnfrsf12a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of CD266 / TweakR antibody

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mR expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.The ITEM-4 antibody has been show to have blocking function.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD266 / TweakR (55-63) antibody

AP22404PU-N CD266/TweakR antibody staining of Human placenta. Rabbit IgG Aff - Purified Hu FN, P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD266 / TweakR (55-63) antibody

AP22404PU-N CD266/TweakR antibody staining of Human placenta. Rabbit IgG Aff - Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD266 / TweakR (55-63) antibody

AP22404PU-N CD266/TweakR antibody staining of Human placenta. Rabbit IgG Aff - Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD266 / TweakR antibody

Western blot analysis of Fn14 in (A) mock- and (B) Fn14-GPI-transfected 293 cells with Fn14 antibody at 1 ug/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD266 / TweakR antibody

Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 1.0 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD266 (TWEAK Receptor) Purified (filled histogram) followed by Anti-Mouse IgG FITC . Total viable cells were analyzed. Mouse ITEM-1 Purified Hu F, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD266 / TweakR antibody

Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 0.5 ug Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC . Total viable cells were analyzed. Mouse ITEM-4 Purified Hu, Ms F, P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD266 / TweakR antibody

Predicted band size: 14 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD266 / TweakR antibody

Predicted band size: 14 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD266 / TweakR antibody

CD266 / TweakR Mouse IgG2b ITEM-4 Low Endotoxin Hu, Ms C, F, FN, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD266 / TweakR antibody

CD266 / TweakR Mouse IgG2b ITEM-4 Purified Hu, Ms C, F, FN, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD266 / TweakR antibody

CD266 / TweakR Mouse IgG2b ITEM-4 PE Hu, Ms F
0.1 mg / €290.00
  Acris Antibodies GmbH

CD266 / TweakR (C-term) antibody

Human renal cell carcinoma stained with anti-Fn14 Antibody Cat.-No AM33209PU (Clone SP134). Rabbit IgG SP134 Purified Frog, Hu, Ms, Rt F, P, WB
0.5 ml / €520.00
  Acris Antibodies GmbH

CD266 / TweakR (C-term) antibody

Human renal cell carcinoma stained with anti-Fn14 Antibody Cat.-No AM33209PU (Clone SP134). Rabbit IgG SP134 Purified Frog, Hu, Ms, Rt F, P, WB
1 ml / €750.00
  Acris Antibodies GmbH

CD266 / TweakR (C-term) antibody

Human renal cell carcinoma stained with anti-Fn14 Antibody Cat.-No AM33209PU (Clone SP134). Rabbit IgG SP134 Purified Frog, Hu, Ms, Rt F, P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

CD266 / TweakR antibody

Figure 2. AP07793PU-N TweakR antibody staining of Formalin-Fixed Paraffin-Embedded Placenta tissue. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD266 / TweakR antibody

CD266 / TweakR Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD266 / TweakR antibody

Detection limit for recombinant GST tagged TNFRSF12A is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1B6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD266 / TweakR antibody

Western blot analysis of TNFRSF12A in 293 cells. Untransfected ( lane 1 ) or transfectd with human TNFRSF12A ( lane 2 ) . Photograph courtesy of Dr. Taly Spivakk, The MD Anderson Cancer Center, Houston, TX. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD266 / TweakR antibody

Immunohistochemistry of TNFRSF12A in human liver tissue with TNFRSF12A polyclonal antibody ( Cat # PAB12972 ) at 2.5 µg/ml. Rabbit Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

CD266 / TweakR antibody

Immunohistochemistry of Fn14 in human liver tissue with Fn14 polyclonal antibody ( Cat # PAB13804 ) at 2.5 µg/mL. Rabbit IgG Hu P, WB
0.1 mg / €820.00
  Abnova Taiwan Corp.

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