CD271 / NGFR antibody

Principal name

CD271 / NGFR antibody

Alternative names for CD271 / NGFR antibody

TNFRSF16, Tumor necrosis factor receptor superfamily member 16

SwissProt ID

P07174 (Rat), P08138 (Human), P18519 (Chick), Q9Z0W1 (Mouse)

Gene ID

4804 (NGFR), 24596 (Ngfr), 4747 (NEFL)

Available reactivities

Fe (Feline), Hu (Human), Por (Porcine), Ms (Mouse), Ft (Ferret), Mky (Monkey), Rb (Rabbit), Rt (Rat), Bab (Baboon), GP (Guinea Pig), Can (Canine), Sh (Sheep), Bov (Bovine), Prim (Primate)

Available hosts

Mouse, Goat, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Immunoelectron Microscopy (EM), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), ELISA (detection) (E(detection)), Neutralisation (NEUT)

Background of CD271 / NGFR antibody

Nerve Growth Factor Receptor, also termed NGFR p75, p75NTR, or CD271 is the low affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is a 75kD transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.). NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. The signal transduction mechanisms and components leading to NGFR p75 multiple signals are complex and not well understood.
In contrast to other members of the neurotrophin receptors family (TrkA, TrkB and TrkC tyrosine kinases), NGFR p75 lacks intrinsic catalytic activity. It has been suggested that NGFR p75 interacts with TrkA to form high affinity binding sites and to modulate TrkA signaling. NGFR p75 belongs to the TNF receptor superfamily which includes TNFR, CD40 and Fas. These receptors all have an intracellular death domain and can couple to parallel signaling pathways leading to apoptotic cell death or activation of the transcription factor NF kappaB. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).

General readings

General / background references:Frade JM & Barde YA Nerve growth factor: two receptors, multiple functions. Bioessays 20:137-45 (1998). PubMed PMID: 9631659
Carter BD et al. Selective activation of NF-kappa B by nerve growth factor through the neurotrophin receptor p75. Science 272:542-5 (1996). PubMed PMID: 8614802
Bothwell M p75NTR: a receptor after all. Science 272:506-7 (1996). PubMed PMID: 8614797
Barinaga M Signaling inside neurons takes some new twists. Science 272:1742-3 (1996). PubMed PMID: 8650570
Van der Zee CE et al. Survival of cholinergic forebrain neurons in developing p75NGFR-deficient mice. Science 274:1729-32 (1996). PubMed PMID: 8939868
DiStefano PS et al. Involvement of a metalloprotease in low-affinity nerve growth factor receptor truncation: inhibition of truncation in vitro and in vivo. J Neurosci 13:2405-14 (1993). PubMed PMID: 8501514
Johnson D et al. Expression and structure of the human NGF receptor. Cell 47:545-54 (1986). PubMed PMID: 3022937

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD271 / NGFR (1-160) antibody

Surface staining (mass cytometry) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets. Mouse IgG1 NGFR5 Purified Fe, Ft, Hu, Mky, Rb F, ICC/IF, IP, P, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD271 / NGFR antibody

Western blot analysis of rat brain membranes: 1. Anti-Human p75NTR (extracellular) antibody, (1:200). 2. Anti-Human p75NTR (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western blot analysis of rat dorsal root ganglion lysates: 1. Anti-Neurotensin Receptor 2 antibody, (1:200). 2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CD271 / NGFR (202-215) antibody

AP31775PU-N NGFR antibody staining of Rat Heart lysate at 1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD271 / NGFR antibody

TA303032 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western blot analysis of (A) 25 ng, (B) 50 ng, and (C) 100 ng of purified recombinant NGFR with NGFR antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Predicted band size: 45 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Predicted band size: 45 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/950 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western blot analysis of extracts from HepG2 cells, using TNR16 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western blot analysis of extracts of various cell lines, using NGFR antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Immunohistochemistry staining of Paraffin Breast tissue by NGFR antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Mouse IgG1 MRQ-21 Purified Hu P
1 ml / €410.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western blot analysis of rat brain membranes: 1. Anti-Human p75 NTR (intracellular) antibody, (1:200). 2. Anti-Human p75 NTR (intracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western Blot: p75NTR/NGF Receptor Antibody (2F1C2) [TA336800] - Western blot analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 2F1C2 Purified Hu E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

Western blot analysis on Jurkat and NIH-3T3 cell lysate using CD271 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR antibody

ELISA: NGF Receptor Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

CD271 / NGFR (1-160) antibody

Surface staining (mass cytometry) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets. Mouse IgG1 NGFR5 Biotin Fe, Ft, Hu, Prim, Rb F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD271 / NGFR antibody

Immunofluorescence analysis of EC cells using NGFR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 2F1C2 Ascites Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CD271 / NGFR antibody

Formalin-Fixed, Paraffin-Embedded Human breast sclerosing adenosis stained with NGFR antibody Cat.-No. AM10123SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of myoepithelial cells. Mouse IgG1 MRQ-21 Supernatant Hu P
1 ml / €500.00
  Acris Antibodies GmbH

CD271 / NGFR antibody

CD271 / NGFR Mouse IgG1 ME20.4 APC Hu F
0.1 mg / €880.00
  Acris Antibodies GmbH

CD271 / NGFR antibody

CD271 / NGFR Mouse IgG1 ME20.4 Biotin Hu C, F, ICC/IF, IP, NEUT, P, WB
0.1 mg / €770.00
  Acris Antibodies GmbH

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