CD274 / PDL1 antibody

Principal name

CD274 / PDL1 antibody

Alternative names for CD274 / PDL1 antibody

PD-L1, PDCD1 ligand 1, B7H1, B7H1, B7 homolog 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1

SwissProt ID

Q9EP73 (Mouse), Q9NZQ7 (Human)

Gene ID

29126 (CD274), 60533 (Cd274)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD274 / PDL1 antibody

Programmed death-ligand 1 (PD-L1), or B7-H1, is a transmembrane, co-stimulatory ligand of programmed cell death protein 1 (PD- 1) that, along with B7-1 and B7-2, belongs to the B7 family and immunoglobulin superfamily. Though more notably expressed on activated Tcells, B-cells, myeloid cells, and a subset of thymocytes, PD-L1 is also expressed constitutively by nonlymphoid, parenchymal organs, including the heart, placenta, skeletal muscle, and lung; with the marked exception of the small intestine. As a member of the B7 family, PD-L1 plays a principal role in immunity: suppressing immune response against autoantigens and tumors, maintaining T-cell homeostasis, maintaining peripheral immune tolerance, and regulating T-cell-mediated cytokine secretion. Unlike B7-1 and B7-2, PD-L1 has not been shown to influence immunity through interaction with CD28, CTL4A or ICOS, but rather through interaction with PD-1, a weak structural homolog of CTLA4 that belongs to the same superfamily. Involvement of PD-1 suggests an inhibitory function during T-cell activation; however, evidence has demonstrated PD-L1’s conflicting responsibility for both the stimulation and inhibition of T-cell-mediated cytokine synthesis. While T-cell co-stimulation with PD-L1 induces proliferation and the secretion of IL-10 and IFN-γ, muscle cell expression of PD-L1 has been shown to inhibit function of CD4 and CD8 Tcells by down-regulating cytokine secretion and the expression of T-cell activation markers. Augmented expression of PD-L1 has been linked to the inhibition of antitumor immune response in cancer, and the up-regulation of IL-10 production in HIV-infection, resulting in increased susceptibility of antigen-specific T-cells to apoptosis.

General readings

Thompson RH, Gillett MD, Cheville JC, Lohse CM, Dong H, Webster WS, Krejci KG, Lobo JR, Sengupta S, Chen L, Zincke H, Blute ML, Strome SE, Leibovich BC, Kwon ED. Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17174-9. Epub 2004 Dec 7. PMID: 15569934

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD274 / PDL1 (C-term) antibody

TA302988 (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

Staining of Mouse peripheral blood lymphocytes with Rat anti Mouse CD274 (Cat.-No AM05612PU-N) Rat IgG2a MIH6 Purified Ms C, F, IP
0.1 mg / €240.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Immunohistochemical detection of CD274 on human 10 weeks placenta paraffin embedded section with AM26531AF-N. Mouse IgG2b 27A2 Azide Free Hu F, P
0.1 mg / €360.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121)(1:25) Mouse IgG1 UMAB229 Aff - Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD274 (PD-L1) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121)(1:25) Mouse IgG1 UMAB229 Aff - Purified Hu P
30 µl / €120.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

Staining of C57Bl/6 splenocytes with 0.25 ug Rat IgG2a K Isotype Control Purified (open histogram) or 0.25 ug Anti-Mouse CD274 (B7-H1) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Rat 1-111A Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

Staining of mouse splenocytes with Anti-Mouse CD274 (B7-H1) PE.  Appropriate isotype controls were used  (open histogram).  Total viable cells were used for analysis. Rat MIH5 Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

Western blot analysis of extracts of human heart cell lines, using CD274 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD274 / PDL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. Mouse IgG1 OTI1B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD274 / PDL1 antibody

Anti-CD274 mouse monoclonal antibody (TA507087) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD274(RC213071). Mouse IgG1 OTI2C7 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD274 / PDL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. Mouse IgG1 OTI4D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD274 / PDL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. Mouse IgG1 OTI2E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD274 / PDL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274. Mouse IgG1 OTI2B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD274 / PDL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.(1:500) Mouse IgG1 OTI9E12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD274 / PDL1 antibody

Staining of Mouse peripheral blood lymphocytes with Rat anti Mouse CD274 antibody -FITC (AM05612FC-N) Rat IgG2a MIH6 FITC Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD274 Antibody Low Endotoxin (Cat.-No AM05612LE-N) Rat IgG2a MIH6 Low Endotoxin Ms C, F, IP
0.5 mg / €560.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

CD274 / PDL1 Rat IgG2a MIH6 Purified Ms C, F, IP
0.25 mg / €390.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Flow Cytometry: AM05613FC-N FITC conjugated CD274 antibody Staining of Human peripheral blood monocytes. Mouse IgG1 MIH2 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274(AM05613PU-L). Mouse IgG1 MIH2 Purified Hu F
0.2 mg / €490.00
  Acris Antibodies GmbH

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