CD275 / ICOS Ligand antibody

Principal name

CD275 / ICOS Ligand antibody

Alternative names for CD275 / ICOS Ligand antibody

B7 homolog 2, B7-like protein Gl50, B7-related protein 1, ICOSLG, B7H2, B7RP1, B7RP-1, ICOSL, KIAA0653

SwissProt ID

O75144 (Human), Q9JHJ8 (Mouse)

Gene ID

23308 (ICOSLG), 50723 (Icosl)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rat, Rabbit

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Western blot / Immunoblot (WB), Frozen Sections (C), Neutralisation (NEUT), EPair for Elisa (EPair), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP)

Background of CD275 / ICOS Ligand antibody

CD275 is a member of the B7 family, and interaction with its receptor CD278 (ICOS) plays an important role in T cell co-stimulation and inflammatory immune reactions.
CD275 is expressed on activated B-cells, monocytes and dendritic cells and is also induced on non-lymphoid tissue cells such as endothelial cells, epithelial cells and muscle cells. CD275 has an important physiological role in the reactivation of effector/memory T cells on the endothelium, thus controlling entry of immune cells into inflamed tissue. The CD278-CD275 pathway is thought to be critical in the immune response to transplants, allergies and autoimmune diseases.

General readings

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wang, K., et al. Hum. Mol. Genet. 19(10):2059-2067(2010)
Pan, F., et al. Immunogenetics 62(4):237-251(2010)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD275 / ICOS Ligand antibody

Fig.2: SDS-PAGE analysis of purified GM-13C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 µg of purified GM-13C1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent. Mouse IgG1 GM13C1 Purified Hu E, F
0.1 mg / €330.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ICOSLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808779)(1:150) Mouse IgG2a OTI2C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand (full length) antibody

Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. Mouse IgG2b 2D3 Aff - Purified Hu E, F, IP, P
50 µg / €345.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ICOSLG antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

WB Suggested Anti-ICOSLG Antibody; Titration: 1.0 ug/ml; Positive Control: ACHN Whole Cell Rabbit IgG Purified Bov, Eq, GP, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ICOSLG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808594)(1:150) Mouse IgG1 OTI2G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG (RC208975, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICOSLG.(1:2000) Mouse IgG1 OTI1D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG (RC208975, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICOSLG.(1:2000) Mouse IgG1 OTI1B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand antibody

Figure 1. Staining of Human peripheral blood monocytes with Mouse anti Human CD275 antibody FITC conjugated (AM05615FC-N). Mouse IgG1 MIH11 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

CD275 / ICOS Ligand Mouse IgG1 MIH11 Purified Hu C, F
0.2 mg / €480.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

Figure 1. Staining of Human peripheral blood monocytes with Mouse anti Human CD275 antibody (AM05615PU-N) Mouse IgG1 MIH11 Purified Hu C, F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand (C-term) antibody

Western blot analysis of CD275 / ICOS Ligand (C-term) Antibody Cat.-No AP52143PU-N in HL-60 cell line lysates (35ug/lane). This demonstrates the ICOSLG antibody detected the ICOSLG protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

Detection limit for recombinant GST tagged ICOSLG is approximately 1ng/ml as a capture antibody. Mouse IgG1 1E2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD275 / ICOS Ligand antibody

Detection limit for recombinant GST tagged ICOSLG is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 2C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD275 / ICOS Ligand antibody

Detection limit for recombinant GST tagged ICOSLG is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 4D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD275 / ICOS Ligand antibody

ICOSLG monoclonal antibody (M01), clone 1E2 Western Blot analysis of ICOSLG expression in HL-60 ( Cat # L014V1 ). Mouse IgG1 2D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD275 / ICOS Ligand antibody

Human monocyte-derived dendritic cells stained with biotinylated 2D3,followed by Sav-PE Mouse IgG2b 2D3 Biotin Hu F
0.1 mg / €360.00
  Novus Biologicals Inc.

CD275 / ICOS Ligand antibody

CD275 / ICOS Ligand Rat IgG2 AP-MAB0838 Purified Ms F, NEUT
0.5 mg / €380.00
  Novus Biologicals Inc.

CD275 / ICOS Ligand antibody

CD275 / ICOS Ligand Mouse IgG2b 2D3 Hu F, P
50 µg / €480.00
  Novus Biologicals Inc.

CD275 / ICOS Ligand antibody

CD275 / ICOS Ligand Rat IgG2a HK5.3 Aff - Purified Ms F
0.1 mg / €360.00
  Novus Biologicals Inc.

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