CD275 / ICOS Ligand antibody

Principal name

CD275 / ICOS Ligand antibody

Alternative names for CD275 / ICOS Ligand antibody

B7 homolog 2, B7-like protein Gl50, B7-related protein 1, ICOSLG, B7H2, B7RP1, B7RP-1, ICOSL, KIAA0653

SwissProt ID

O75144 (Human), Q9JHJ8 (Mouse)

Gene ID

102723996 (LOC102723996), 50723 (Icosl), 23308 (ICOSLG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Western blot / Immunoblot (WB), Frozen Sections (C), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CD275 / ICOS Ligand antibody

Kit Component:
- KN308076G1, Icosl gRNA vector 1 in pCas-Guide vector
- KN308076G2, Icosl gRNA vector 2 in pCas-Guide vector
- KN308076D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wang, K., et al. Hum. Mol. Genet. 19(10):2059-2067(2010)
Pan, F., et al. Immunogenetics 62(4):237-251(2010)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD275 / ICOS Ligand antibody

Fig.2: SDS-PAGE analysis of purified GM-13C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 µg of purified GM-13C1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent. Mouse IgG1 GM13C1 Purified Hu E, F
0.1 mg / €360.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

  ICOSLG mouse monoclonal antibody, clone OTI2C5 (formerly 2C5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ICOSLG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808779)(1:150) Mouse IgG2a OTI2C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand (full length) antibody

  ICOSL / B7-H2 Mouse Monoclonal (2D3) Antibody  
Anti-ICOSLG / ICOSL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. Mouse IgG2b 2D3 Aff - Purified Hu E, F, IP, P
50 µg / €400.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

  Anti-ICOSLG Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ICOSLG antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

  Rabbit Polyclonal Anti-ICOSLG Antibody  
WB Suggested Anti-ICOSLG Antibody; Titration: 1.0 ug/ml; Positive Control: ACHN Whole Cell Rabbit IgG Purified Bov, Eq, GP, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

  Rabbit Polyclonal Anti-ICOSLG Antibody  
Gel: 6%SDS-PAGE, Lysate: 80 ug, Lane 1-2: Human fetal brain tissue, human lymphoma tissue, Primary antibody: (ICOSLG Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

  Mouse Monoclonal anti-B7-H2(CD275, ICOSL) Antibody  
Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE Mouse 2D3 Purified Hu E, F, IP
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD275 / ICOS Ligand antibody

  ICOSLG mouse monoclonal antibody, clone OTI2G10 (formerly 2G10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ICOSLG mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808594)(1:150) Mouse IgG1 OTI2G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand antibody

  ICOSLG mouse monoclonal antibody, clone OTI1D10 (formerly 1D10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG (RC208975, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICOSLG.(1:2000) Mouse IgG1 OTI1D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand antibody

  ICOSLG mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICOSLG (RC208975, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICOSLG.(1:2000) Mouse IgG1 OTI1B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD275 / ICOS Ligand antibody

Figure 1. Staining of Human peripheral blood monocytes with Mouse anti Human CD275 antibody FITC conjugated (AM05615FC-N). Mouse IgG1 MIH11 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

CD275 / ICOS Ligand Mouse IgG1 MIH11 Purified Hu C, F
0.2 mg / €480.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand antibody

Figure 1. Staining of Human peripheral blood monocytes with Mouse anti Human CD275 antibody (AM05615PU-N) Mouse IgG1 MIH11 Purified Hu C, F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD275 / ICOS Ligand (C-term) antibody

Western blot analysis of CD275 / ICOS Ligand (C-term) Antibody Cat.-No AP52143PU-N in HL-60 cell line lysates (35ug/lane). This demonstrates the ICOSLG antibody detected the ICOSLG protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Icosl - mouse gene knockout kit via CRISPR

Icosl - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Icosl - mouse gene knockout kit via CRISPR

Icosl - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Icosl - mouse gene knockout kit via CRISPR

Icosl - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD275 / ICOS Ligand

  Recombinant protein of human inducible T-cell co-stimulator ligand (ICOSLG)  
CD275 / ICOS Ligand Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CD275 / ICOS Ligand

  Recombinant protein of human inducible T-cell co-stimulator ligand (ICOSLG)  
CD275 / ICOS Ligand Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ICOSLG (mGFP-tagged) - Human inducible T-cell co-stimulator ligand (ICOSLG), 200 uL, >10^7 TU/mL

Lenti ORF particles, ICOSLG (mGFP-tagged) - Human inducible T-cell co-stimulator ligand (ICOSLG), 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

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