CD279 / PD1 antibody
Principal name
CD279 / PD1 antibody
Alternative names for CD279 / PD1 antibody
Protein PD-1, hPD-1, PDCD1, Programmed cell death protein 1
Ncbi ID
9606, NP_005009.2, NP_032824.1, NP_005009, NP_032824, AAH74740.1, NM_005018, Q15116
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of CD279 / PD1 antibody
The J43 monoclol antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
General readings
1. Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90.
2. Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw. 2000; 11:483-6.
3. Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol. 2004; 5:373-9.
4. Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor
accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. 2007; 179:2551-5.
5. Carriere V, Roussel L, Ortega N, et al. IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo. Proc. Natl. Acad. Sci. USA 2007; 104:282-7.
PDCD1 MaxPab polyclonal antibody. Western Blot analysis of PDCD1 expression in human stomach.
Western Blot analysis of PDCD1 expression in transfected 293T cell line (H00005133-T01) by PDCD1 MaxPab polyclonal antibody.Lane 1: PDCD1 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
PDCD1 MaxPab polyclonal antibody. Western Blot analysis of PDCD1 expression in HeLa.
Cell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysisCell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
AP07336PU-N PDCD1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small Intestine.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Skin.
PDCD1 polyclonal antibody ( Cat # PAB6790 ) ( 0.6 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis cell lysates of 293T and U937 ( 40 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with PDCD1 monoclonal antibody , clone 4F12 ( 1 : 500 ) ( Cat # MAB1117 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
PDCD1 MaxPab polyclonal antibody. Western Blot analysis of PDCD1 expression in human stomach.
Western Blot analysis of PDCD1 expression in transfected 293T cell line (H00005133-T01) by PDCD1 MaxPab polyclonal antibody.Lane 1: PDCD1 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
PDCD1 MaxPab polyclonal antibody. Western Blot analysis of PDCD1 expression in HeLa.
Western blot using PDCD1 polyclonal antibody ( Cat # PAB11249 ) shows detection of a predominant band at ~47 kDa corresponding to PDCD1 ( arrowhead ) in THP-1 whole cell lysate.The predicted MW of PDCD1 is 32 kDa.PDCD1 was probed in the absence ( lane A ) and presence ( lane B ) of blocking peptide.
Immunohistochemistry of PDCD1 in human brain tissue with PDCD1 polyclonal antibody ( Cat # PAB13253 ) at 5 µg/ml.
Western blot analysis of PDCD1 in THP-1 cell lysate with PDCD1 polyclonal antibody ( Cat # PAB13253 ) at 1 µg/ml in the ( A ) absence and ( B ) presence of blocking peptide.
AP23806PU CD279 antibody staining of Human Tonsil.
Cell Source: Con A/IL2 activated T-Blasts from Balb/c spleen. Percentage of cells stained above control: 36.6%
Cell Source: Con A/IL2 activated T-Blasts from Balb/c spleen Percentage of cells stained above control: 36.6%
Flow Cytometric analysis of PD-1 expression on X 63 (left) and hPD-1/X 63 (right). Open histograms Indicate the reaction of isotypic control to the cells. Shaded Histograms indicate the reaction of AM26479AF-N to the cells.
Flow cytometric analysis of PD-1 expression on Human periferal blood Lymphocytes (1), Monocytes (2), and Granulocytes (3). Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26479AF-N.
Flow cytometric analysis of PD-1 expression on X63 cells (left) and PD-1 transfected X63 cells (right). Open histogram indicates the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26479RP-N to the cells.
Western blot analysis showing 1 μg/well of recombinant Human PD-1 detected with 1 μg/ml of CD279 / PD1 Antibody Cat.-No AM33411PU (Clone 7C9).
Western blot analysis showing 1 μg/well of recombinant Human PD-1 detected with 1 μg/ml of CD279 / PD1 Antibody Cat.-No AM33412PU-N (Clone 7C9).
Western blot analysis of CD279 expression in THP1 (A); HepG2 (B); mouse liver (C); H9C2 (D) whole cell lysates.
Representative Standard Curve
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806806)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806806)(Left) . The rihgt is negative control.(1:100)(1:900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA806806), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806806) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806806) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806807)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806807) (Red) compared to a nonspecific negative control antibody (Blue).(1:100)(1:50)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806808)(1:150)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806808 (green) and nucleus with Hoechst33342 (blue) . HEK293T cells serve as negative control (right). (1:100)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806808)(Left) . The rihgt is negative control.(1:100)(1:900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PDCD1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA806809)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDCD1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA806809)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806810)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806810 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806810)(Left) . The rihgt is negative control.(1:100)(1:900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA806810), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806810) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806810) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806811)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806811 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806811)(Left) . The rihgt is negative control.(1:100)(1:900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806813)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806813 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806813) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806834)(1:150)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806834 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806834)(Left) . The rihgt is negative control.(1:100)(1:900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806835)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806835 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA806835), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806835) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806835) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806837)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806837 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806837)(Left) . The rihgt is negative control.(1:100)(1:900)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA806837), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806837) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806837) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806927)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806927 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806927) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806927) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806929)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806929 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806929) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806929) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA806929) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806940)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806940 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806940) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806940) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806961)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806961 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA806961) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806961) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA806961) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807094) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807094) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807094) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807098)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807098 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807098) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807098) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807207)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807207 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807207) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807207) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807847) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807847) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807851 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA807851), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807851) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807851) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807851) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807861)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807861 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA807861), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807861) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807861) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807861) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807862) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807862) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807864) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA807864), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807864) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807864) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807865)(Left) . The rihgt is negative control.(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807865 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA807865), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807865) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807865) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807865) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807866) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807866) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807866) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807867)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807867 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807867) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807867) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807867) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807868)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807868 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807868) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807868) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807868) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807879)(Left) . The rihgt is negative control.(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807879 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA807879), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807879) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807879) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807879) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807880) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807880) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807888) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807888) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807889 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA807889), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807889) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA807889) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807889) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDCD1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807942)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807988)(Left) . The rihgt is negative control.(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807988 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807988) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807988) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807995) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807995) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA808002 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA808002)(Left) . The rihgt is negative control.(1:900)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808002) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA808002) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808033) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA808033) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA808033) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808034) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA808034) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA808034) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808035) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA808036)(Left) . The rihgt is negative control.(1:100)(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA808036 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA808036), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808036) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA808036) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA808036) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808043) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA808043), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry.(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA808043) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Flow cytometric Analysis of PDL2(RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody(TA808043) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA808069)(Left) . The rihgt is negative control.(1:900)
Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA808069 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:500)
Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA808069) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA808069) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc(human) IgG-FITC.(1:50)
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody (UM800090). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody (UM800091). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
TA303025 (0.6µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of PDCD1 in human brain tissue with PDCD1 antibody at 2.5 ug/ml.
Immunofluorescence of PD-1 in Human Brain tissue with PD-1 antibody at 20 ug/mL.
Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of PDCD1 in human brain tissue with PDCD1 antibody at 5 ug/ml.
Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking recombinant protein.
Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 ug/mL.
Immunofluorescence of PD-1 in mouse brain tissue with PD-1 antibody at 20 ug/mL.
Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 ug/mL.
Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 ug/mL.
Staining of human PD-1 transfected cells with anti-human Anti-Human CD279 (PD-1) FITC (left)or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin and Streptavidin PE . Total viable cells were used for analysis.
Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Purified Rat IgG2b Isotype Control (open histogram) or 0.25 ug of Anti-Mouse CD279 (PD-1) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysis.
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using PDCD1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PDCD1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Con A (3-day) activated C57BL/6 mouse splenocytes stained with RMP1-30 PE
Human Tonsil stained with anti-PD-1_CD279 antibody.
- Quick-Filters:
- Highly Validated (33)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM800089 | CD279 / PD1 antibody |
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| PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB197 | |||||||
 |
Mouse | IgG2a | UMAB197 | Purified | Hu | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
Highly validated antibody!
|
|||||||
| UM800090 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB198 | |||||||
 |
Mouse | IgG2a | UMAB198 | Purified | Hu | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
Highly validated antibody!
|
||||||
| UM800091 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB199 | |||||||
 |
Mouse | IgG2a | UMAB199 | Purified | Hu | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
Highly validated antibody!
|
||||||
| UM870089 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB197 | |||||||
|
Mouse | IgG2a | UMAB197 | Purified | Hu | P |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
Highly validated antibody!
|
|||||||
| UM870090 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB198 | |||||||
|
Mouse | IgG2a | UMAB198 | Purified | Hu | P |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
Highly validated antibody!
|
||||||
| UM870091 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB199 | |||||||
|
Mouse | IgG2a | UMAB199 | Purified | Hu | P |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
Highly validated antibody!
|
||||||
| TA806806 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI4F10 (formerly 4F10) | |||||||
 |
Mouse | IgG1 | OTI4F10 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA806808 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI8H8 (formerly 8H8) | |||||||
 |
Mouse | IgG2a | OTI8H8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA806810 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI8H7 (formerly 8H7) | |||||||
 |
Mouse | IgG2a | OTI8H7 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA806811 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI11B2 (formerly 11B2) | |||||||
 |
Mouse | IgG2a | OTI11B2 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA806813 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI12D11 (formerly 12D11) | |||||||
 |
Mouse | IgG2a | OTI12D11 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA806834 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI2F6 (formerly 2F6) | |||||||
 |
Mouse | IgG1 | OTI2F6 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA806835 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI8B1 (formerly 8B1) | |||||||
 |
Mouse | IgG1 | OTI8B1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA806837 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI4E5 (formerly 4E5) | |||||||
 |
Mouse | IgG1 | OTI4E5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
| TA806927 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI21F5 | |||||||
 |
Mouse | IgG2a | OTI21F5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA806929 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI16D4 | |||||||
 |
Mouse | IgG1 | OTI16D4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA806940 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI14C5 | |||||||
 |
Mouse | IgG2b | OTI14C5 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA806961 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI14B3 | |||||||
 |
Mouse | IgG1 | OTI14B3 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA807098 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI26C4 | |||||||
 |
Mouse | IgG1 | OTI26C4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA807207 | CD279 / PD1 antibody |
||||||
| PD-1 (PDCD1) mouse monoclonal antibody, clone OTI17G9 | |||||||
 |
Mouse | IgG1 | OTI17G9 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
