CD281 / TLR1 antibody

Principal name

CD281 / TLR1 antibody

Alternative names for CD281 / TLR1 antibody

Toll-like receptor 1, TIL, KIAA0012, Toll/interleukin-1 receptor-like protein

SwissProt ID

Q15399 (Human), Q9EPQ1 (Mouse)

Gene ID

7096 (TLR1), 21897 (Tlr1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), Functional assay (FN)

Background of CD281 / TLR1 antibody

The Toll-like receptor (TLR) multigene family encodes important recognition receptors of the innate immune system that have been conserved in both the invertebrate and vertebrate lineages . The mammalian host defense system is essentially regulated by these conserved Toll-like receptors. At least 13 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens and activation of innate immunity. TLRs were identified as genes coding for both an N-terminal leucine-rich repeat (LRR) domain and a C-terminal Toll-IL-resistance (TIR) domain. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM) and caused production of inflammatory cytokines such as IL-1, IL-6, IL-8 , TNFa, and IL-12, chemokines and co-stimulatory molecules such as CD40, CD80 and CD86. In the presence of inflammatory cytokines and binding of adaptor molecule, MyD88 that binds FADD and triggers apoptosis through the caspase cascade. TLR induced apoptosis pathway appears to be a repertoire of defense mechanism utilized by innate defense mechanism. The constitutive expression of many human TLRs (1, 2, 3) have been shown on the surface of myeloid lineage cells by RT-PCR and use of specific monoclonal antibodies. Upon activation of these receptors by their respective chemokines and ligands have been shown in literature on various cell lines including endothelial, epithelial and other cells. The expression of TLR 3, 7, 8 and 9 are mainly found on endosomal lysosomal compartments. Human TLR3 is expressed in human fibroblasts cells and TLR 9 in in-vitro derived DC cells. There is significant evidence of TLR involvement is many systemic disorders following bacterial infection including sepsis, peridontitis, cardiac ischemia, cerebral palsy and others, understanding the TLRs involvement in these conditions will allow therapeutic interventions at the receptor level for treatment of these disorders.
The TLR are highly conserved protein and share structural and functional domains across species. These receptors recognize pathogen associated molecular patters (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines needed for production of immediate immunity. The cell-surface TLRs, including TLR1, TLR2, TLR4, and TLR6, recognize microbial membrane lipids, whereas TLR3, TLR7, TLR8, and TLR9 recognize pathogen-derived nucleotides in intracellular compartments. TLR7 and TLR9 respond to host-derived nucleotides as well, and they have been implicated in a variety of autoimmune diseases. Toll-like receptor 9 (TLR9) is a receptor for unmethylated CpG dinucleotides found in bacterial and viral DNA. TLR11-13 and TLR21-23 ubfamilies are represented by pseudogenes in humans. TLR11 is an ancient gene and is found in lower animnal. The TLR11 receptors recognize uropathogenic bacteria antigens (4). TLR7 is a membrane bound protein with two TMD. The first human toll like receptor to be identified, TLR4, senses lipopolysaccharide (LPS) while TLR2 on the other hand senses diacylated or triacylated lipopeptides after heterodimerizing with either TLR6 or TLR1, respectively. The conserved cytoplasmic TIR (Toll/IL-1 receptor) domains of the IL-1 and Toll-like receptors are the critical focal point for the generation of ligand-induced cytoplasmic signaling cascades. For signaling all the TLRs utilize one or more of the four known TIR-containing adaptor molecules: MyD88, TIRAP/MAL, TRIF, and TRAM. Human TLR9 receptor is expressed as A and B isoforms with apparent MW 125kDa (1032 aa) and 120kDa (975) with several splice variants. TLR antibody will label both A and B isoforms. TLR9 is a trans-membrane receptor protein characterized by extracellular domains with leucine-rich repeats and a cytoplasmic domain with type I IL-1 receptor. The TLR11is expressed on cell surface of peripheral blood mononuclear cells along with major histocaompatibility comples (MHC) class II CD19 positive cells. Many specific adapter molecules (TICAM, MyD88, ICAM2, TRAM, TIRAP, TRIF) are involved in signaling of TLRs.

General readings

1, Wyllie, D et al; Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses. J Immunol 2000, 165: 7125.
2. Visintin, A et al; Regulation of Toll-like receptors in human monocytes and dendritic cells. J Immunol 2001, 166: 249.
3. Sandor, F et al; Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkB signaling. JCB 2003, 162: 1099.
4. Ochoa, M et al; Distribution of Toll-like receptor 1 and Toll-like receptor 2 in human lymphoid tissue. Immunology 2003, 108: 10.
5. Ritter, M et al; Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm 2005, 2: 16.
6. Månsson, A et al; A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by Pam3CSK4, R-837 and CpG-2006 stimulation. Immunology 2006, 118: 539.
7. Panda, A et al; Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol 2010, 184: 2518.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD281 / TLR1 (N-term) antibody

Immunohistochemistry of APG7 in MCF7 cells with APG7 antibody at 10 ug/ml. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD281 / TLR1 (Center) antibody

Western blot analysis of TLR1 in Daudi cell lysate with TLR1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD281 / TLR1 antibody

Left:  Untransfected control (left) and mouse TLR1-transfected (right) Baf/3 cell lysates were loaded at 1x10e5 cells/lane, probed with 3 ug/mL Anti-Mouse CD281 (TLR1) Purified and revealed with HRP anti-rat IgG. Right:  Peritoneal-exudate (left) and RAW264.7 (right) cell lysates were loaded at 1x10e5 cells/lane, probed with 3 ug/ml Anti-Mouse CD281 (TLR1) Purified and revealed with HRP anti-rat IgG. Rat eBioLD5 Purified Ms WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD281 / TLR1 antibody

Staining of normal human peripheral blood cells with Anti-Human CD281 (TLR1) Purified vs. Purified Mouse IgG1 Isotype Control . Cell sin the monocyte gate were used for analysis. Mouse GD2.F4 Purified Hu F, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD281 / TLR1 antibody

Predicted band size: 90 kDa . Positive control: Mouse thymus tissue and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: Mouse thymus tissue lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/175 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD281 / TLR1 antibody

Western blot analysis of extracts of various cell lines, using TLR1 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD281 / TLR1 antibody

Western Blot: TLR1 Antibody [TA336407] - analysis of TLR1 in Ramos (A), Raw (B), and © TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used. Rabbit Purified Hu, Ms, Rt F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD281 / TLR1 antibody

CD281 / TLR1 Mouse IgG1 GD2.F4 Biotin Hu C, E, F, ICC/IF, P
50 µg / €280.00
  Acris Antibodies GmbH

CD281 / TLR1 antibody

CD281 / TLR1 Mouse IgG1 GD2.F4 FITC Hu C, F, ICC/IF, P
0.1 mg / €390.00
  Acris Antibodies GmbH

CD281 / TLR1 antibody

CD281 / TLR1 Mouse IgG1 GD2.F4 Purified Hu C, E, F, FN, ICC/IF, P
0.1 mg / €340.00
  Acris Antibodies GmbH

CD281 / TLR1 antibody

CD281 / TLR1 Goat Aff - Purified Hu E, WB
0.1 ml / €390.00
  Acris Antibodies GmbH

CD281 / TLR1 (N-term.) antibody

Western blot analysis of TLR1 in mouse spleen lysate with TLR1 antibody at (A) 1, (B) 2, and (C) 4 µg/ml Rabbit IgG Purified Hu, Ms C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CD281 / TLR1 (N-term) antibody

Immunohistochemical staining of mouse spleen with Rabbit anti Human CD281(AP05596PU-N) Rabbit IgG Purified Hu, Ms C, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD281 / TLR1 antibody

Figure 1. AP07365PU-N CD281/TLR1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small Intestine. Rabbit Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD281 / TLR1 antibody

Figure 1. Immunohistochemical staining (FFPE) of Small Intestine tissue. Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD281 / TLR1 Control Peptide

CD281 / TLR1
0.1 mg / €250.00
  Acris Antibodies GmbH

CD281 / TLR1 antibody

CD281 / TLR1 Rabbit IgG Aff - Purified Hu Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD281 / TLR1 (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

CD281 / TLR1 Control Peptide

CD281 / TLR1
50 µg / €160.00
  Acris Antibodies GmbH

CD281 / TLR1 Control Peptide

CD281 / TLR1
50 µg / €160.00
  Acris Antibodies GmbH

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