CD282 / TLR2 antibody

Principal name

CD282 / TLR2 antibody

Alternative names for CD282 / TLR2 antibody

Toll-like receptor 2

SwissProt ID

B2LT65 (Sheep), O60603 (Human), Q95LA9 (Bovin), Q9QUN7 (Mouse)

Gene ID

7097 (TLR2), 24088 (Tlr2), 7097979 (accA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Bov (Bovine)

Available hosts

Mouse, Rat, Rabbit, Chicken, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ChIP assay (ChIP)

Background of CD282 / TLR2 antibody

The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single a-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.

General readings

1. Kajava AV, Vasselon T. A network of hydrogen bonds on the surface of TLR2 controls ligand positioning and cell signaling. J Biol Chem. 26 February 2010; 285(9):6227-6234.
2. Kaisho T, Akira S. Toll-like receptors as adjuvant receptors. Biochim Biophys Acta. 13 February 2002; 1589(1):1-13.
3. Wetzler LM. The role of Toll-like receptor 2 in microbial disease and immunity. Vaccine. 1 June 2003; 21(2):555-560.
4. Satta N, et al. Induction of TLR2 expression by inflammatory stimuli is required for endothelial cell responses to lipopeptides. Mol Immunol. November 2008;
46(1):145-157.
5. Sallustio F, et al. TLR2 plays a role in the activation of human resident renal stem/progenitor cells. FASEB J. February 2010; 24(2):514-525.
6. Goto Y, et al. Activation of Toll-like receptors 2, 3 and 4 on human melanoma cells induces inflammatory factors. Mol Cancer Ther. November 2008; 7(11):3642-3653.
7. Stadlbauer V, et al. Role of Toll-like receptors 2, 4 and 9 in mediating neutrophil dysfunction in alcoholic hepatitis. Am J Physiol Gastrointest Liver Physiol. January
2009; 296(1):G15-G22.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD282 / TLR2 antibody

Human liver; . Sample Type: Human Macrophange Cells. Green: primary. Red: phallodin. Blue: DAPI. Yellow: green/red. Primary Dilution: 1:200. Secondary Antibody: anti-Rabbit IgG-FITC. Secondary Dilution: 1:1000. Image Submitted By: Milan Fiala. University of California, Los Angeles .; TLR2 antibody - C-terminal region (ARP30551_P050) in Human liver cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €350.00
  AVIVA Systems Biology

CD282 / TLR2 (C-term) antibody

CD282 / TLR2 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
20 µl / €50.00
  SICGEN

CD282 / TLR2 (C-term) antibody

CD282 / TLR2 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €300.00
  SICGEN

CD282 / TLR2 (N-term) antibody

Western blot analysis of TLR2 in A20 cell lysates with TLR2 antibody at 1 ug/ml in the presence (A) and absence (B) of its blocking peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Staining of Raw246.7 cells with 0.25 ug of Mouse IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD282 (TLR2) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Mouse mT2.7 Purified Ms F, IP, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Staining of RAW264.7 cells with 0.25 ug Purified Mouse IgG  Isotype Control (open histogram) or 0.25 ug Anti-Human/Mouse CD282 (TLR2) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Mouse T2.5 Purified Hu, Ms F, IP, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Human peripheral blood monocytes were stained with Anti-Human CD282 (TLR2) Purified followed by Anti-Mouse IgG FITC . (Gated on normal peripheral monocytes) Mouse TL2.1 Purified Hu F, IP, P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 1.0 ug of Anti-Human CD282 (TLR2) Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Cells in the monocyte gate were used for analysis. Mouse TL2.3 Purified Hu F, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using TLR2 antibody at dilution 1/55, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Western Blot: TLR2 Antibody [TA336413] - Analysis of TLR2 in (A) 15 ugs of Raw cell lysate (B) Mouse TLR2 transfected cell lysate and © Ramos cell lysate. 1 ug/ml. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Western Blot: TLR2 Antibody [TA336461] - analysis of TLR2 in transfected cell lysate using this antibody. Rabbit Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Flow Cytometry: TLR2 Antibody (TL2.1) [TA336462] - Cell surface flow analysis of TLR2 in 10^6 PBMCs using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR2 antibody. Mouse IgG2a TL2.1 Purified Can, Hu F, ICC/IF, IP, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Western Blot: TLR2 Antibody (1030A5.138) [TA336466] - Analysis of TLR2 in 30 ugs of Ramos whole cell lysate using TLR2 antibody at 1:500. Mouse IgG1 1030A5.138 Purified Hu F, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Western Blot: TLR2 Antibody (11G5) [TA337143] - analysis of TLR2 antibody. Mouse RAW cell lysate was probed using TLR2 antibody at 5 ug/ml.  goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Rat IgG2a 11G5 Purified Ms F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD282 / TLR2 antibody

Western blot analysis of TLR2 in Ramos cell lysate. Mouse IgG1 BV31-9 Purified Hu WB
0.1 ml / €420.00
  Acris Antibodies GmbH

CD282 / TLR2 antibody

CD282 / TLR2 Rat IgG2b 6C2 Biotin Ms F
50 µg / €280.00
  Acris Antibodies GmbH

CD282 / TLR2 antibody

CD282 / TLR2 Rat IgG2b 6C2 FITC Ms F, ICC/IF
0.1 mg / €390.00
  Acris Antibodies GmbH

CD282 / TLR2 antibody

CD282 / TLR2 Rat IgG2a 6C2 Purified Ms F, IP
0.25 mg / €500.00
  Acris Antibodies GmbH

CD282 / TLR2 antibody

CD282 / TLR2 Rat IgG2b 6C2 Purified Ms F, ICC/IF, IP
0.1 mg / €340.00
  Acris Antibodies GmbH

CD282 / TLR2 antibody

CD282 / TLR2 Rat IgG2a 6C2 Purified Ms F, IP
25 µg / €190.00
  Acris Antibodies GmbH

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