CD284 / TLR4 antibody

Principal name

CD284 / TLR4 antibody

Alternative names for CD284 / TLR4 antibody

Toll-like receptor 4

SwissProt ID

O00206 (Human), Q68Y56 (Pig), Q9GL65 (Bovin), Q9MYW3 (Horse), Q9QUK6 (Mouse), Q9QX05 (Rat)

Gene ID

7099 (TLR4), 21898 (Tlr4), 29260

Available reactivities

Hu (Human), Can (Canine), GP (Guinea Pig), Mky (Monkey), Por (Porcine), Ms (Mouse), Rt (Rat), Bov (Bovine), Sq (Squirrel), Prim (Primate)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Neutralisation (NEUT)

Background of CD284 / TLR4 antibody

The mammalian host defense system is essentially regulated by highly conserved Toll-like receptor (TLR) family of proteins. At least 13 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens and activation of innate immunity. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM) and caused production of inflammatory cytokines such as IL-1, IL-6, IL-8 , TNFa, and IL-12, chemokines and co-stimulatory molecules such as CD40, CD80 and CD86. In the presence of inflammatory cytokines and binding of adaptor molecule, MyD88 that binds FADD and triggers apoptosis through the caspase cascade. TLR induced apoptosis pathway appears to be a repertoire of defense mechanism utilized by innate defense mechanism. The constitutive expression of many human TLRs (1, 2, 3) have been shown on the surface of myeloid lineage cells by RT-PCR and use of specific monoclonal antibodies. Upon activation of these receptors by their respective chemokines and ligands have been shown in literature on various cell lines including endothelial, epithelial and other cells. The expression of TLR 3, 7, 8 and 9 are mainly found on endosomal lysosomal compartments. Human TLR3 is expressed in human fibroblasts cells and TLR 9 in in-vitro derived DC cells. There is significant evidence of TLR involvement is many systemic disorders following bacterial infection including sepsis, peridontitis, cardiac ischemia, cerebral palsy and others, understanding the TLRs involvement in these conditions will allow therapeutic interventions at the receptor level for treatment of these disorders.
The TLR are highly conserved protein and share structural and functional domains across species. These receptors recognize pathogen associated molecular patters (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines needed for production of immediate immunity. TLR4 signaling pathway are associated with expression of pro-inflammatory cytokines and activation of the PI3K/Akt signaling pathway in the hippocampal formation and is involved in ischemia-induced perfusion hippocampal neuronal death (4). Human TLR4 is a 839 amino acid (105 kDa) type I trans-membrane receptor protein characterized by extracellular domains with leucine-rich repeats and a cytoplasmic domain with type I IL-1 receptor. TLR4 deficiency led to the upregulation of what we believe to be a novel NADPH oxidase (Nox), Nox3, in lungs and endothelial cells, resulting in increased oxidant generation and elastolytic activity (5). The TLR 2 and 4 are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and is involved in innate immunity. TLR4 is expressed on peripheral blood and together with MD-2 and CD14 is responsible for LPS signaling recognition. Many specific adapter molecules (TICAM, MyD88, ICAM2, TRAM, TIRAP, TRIF etc) are also involved in signaling of several other TLRs.

General readings

General / background references:Jiang Q et al. Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B. J Immunol 165:3541-4 (2000). PubMed PMID: 11034352
Shimazu R et al. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med 189:1777-82 (1999). PubMed PMID: 10359581

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD284 / TLR4 antibody

Western Blot: TLR4 Antibody (76B357.1) [TA336410] - analysis of TLR4 antibody using TLR4 antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate. Mouse IgG2b 76B357.1 Purified Hu, Ms, Rt, Sq C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 (N-term) antibody

Western blot analysis of TLR4 in PC-3 cell lysates with TLR4 antibody at (A) 2.5 and (B) 5 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western blot analysis of TLR4 using a 1:1000 dilution of TA309486. Rabbit Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western blot analysis of TLR4 in rat kidney tissue lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 (C-term) antibody

Immunohistochemistry of TLR4 in human placenta tissue with TLR4 antibody at 5 ug/mL. Rabbit IgG Aff - Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Surface staining of normal human peripheral blood cells with Anti-Human CD284 (TLR4) PE (left), and Anti-Human CD284 (TLR4) APC(right).  Appropriate isotype controls were used (open histogram).  Cells in the monocyte population were used for analysis. Mouse HTA125 Purified Hu F, IP, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western blot analysis of extracts of HL-60 cell lines, using TLR4 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

CD284 / TLR4 Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Flow Cytometry: TLR4 Antibody (MTS510) [TA336405] - Cell surface analysis of TLR4 on mouse peritoneal cells using 1 ug/10^6 cells of TLR4 anitbody, anti-ratPE conjugated secondary and TLR cell surface flow kit (green represents isotype control; red repres Rat IgG2a MTS510 Purified Ms F, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western Blot: TLR4 Antibody [TA336414] - analysis of TLR4 using this antibody. 1 ug/lane partial recombinant mouse TLR4 protein (tested at 2 ug/ml) and B) RAW cell lysate and C) Daudi cell lysate (tested at 5 ug/ml on tumor cell lysate). Rabbit Purified Hu, Ms, Prim ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western Blot: TLR4 Antibody [TA336415] - Analysis of TLR4 using NB 100-56581 at 2 ug/ml on partial recombinant mouse TLR4 protein. Rabbit Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Flow Cytometry: TLR4 Antibody (HTA125) [TA336463] - Analysis of TLR4 using TA336463 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC. Mouse IgG2a HTA125 Purified Can, Hu F, ICC/IF, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western Blot: TLR4 Antibody [TA336848] - Analysis of TLR4 in Raw 264.7 cell lysate. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western Blot: TLR4 Antibody [TA337068] - Analysis of TLR4 using TLR4 antibody at 2 ug/ml on partial recombinant mouse TLR4 protein. Rabbit IgG Purified Hu, Ms C, F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Western Blot: TLR4 [p Tyr674] Antibody [TA337086] - Analysis of phospho TLR4 in (A) recombinant fusion protein containing Tyr674 and (B) fusion partner without the phospho amino acid, using phospho TLR4 antibody at 0.01 ug/ml. Rabbit Purified Hu, Ms, Rt WB
20 µg / €325.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Anti-TLR4 antibody IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu, Mky
50 µg / €379.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

Anti-TLR4 antibody IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

CD284 / TLR4 antibody

CD284 / TLR4 Mouse IgG2a 1E11 Purified Hu E, F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD284 / TLR4 (238-609) antibody

AM08389AF-N CD284/TLR4 Antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 1E11 Azide Free Hu E, F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD284 / TLR4 antibody

CD284 / TLR4 Mouse IgG1 3C3 Biotin Hu F
50 µg / €280.00
  Acris Antibodies GmbH

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