CD289 / TLR9 antibody

Principal name

CD289 / TLR9 antibody

Alternative names for CD289 / TLR9 antibody

Toll-like receptor 9, UNQ5798/PRO19605

SwissProt ID

Q5I2M3 (Pig), Q5I2M4 (Sheep), Q5I2M5 (Bovin), Q5I2M7 (Felca), Q5I2M8 (Canfa), Q9EQU3 (Mouse), Q9NR96 (Human)

Gene ID

54106 (TLR9), 81897 (Tlr9)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Chimp (Chimpanzee), Bov (Bovine), Por (Porcine), Prim (Primate), Fe (Feline), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Chicken, Rabbit, Goat, Rat

Available applications

Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of CD289 / TLR9 antibody

The mammalian host defense system is essentially regulated by highly conserved Toll-like receptor (TLR) family of proteins. At least 13 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens and activation of innate immunity. The Toll signaling to NF-Kb starts form conserved Toll-IL-1-resistance (TIR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM) and caused production of inflammatory cytokines such as IL-1, IL-6, IL-8 , TNFa, and IL-12, chemokines and co-stimulatory molecules such as CD40, CD80 and CD86. In the presence of inflammatory cytokines and binding of adaptor molecule, MyD88 that binds FADD and triggers apoptosis through the caspase cascade. TLR induced apoptosis pathway appears to be a repertoire of defense mechanism utilized by innate defense mechanism. The constitutive expression of many human TLRs (1, 2, 3) have been shown on the surface of myeloid lineage cells by RT-PCR and use of specific monoclonal antibodies. Upon activation of these receptors by their respective chemokines and ligands have been shown in literature on various cell lines including endothelial, epithelial and other cells. The expression of TLR 3, 7, 8 and 9 are mainly found on endosomal lysosomal compartments. Human TLR3 is expressed in human fibroblasts cells and TLR 9 in in-vitro derived DC cells. There is significant evidence of TLR involvement is many systemic disorders following bacterial infection including sepsis, peridontitis, cardiac ischemia, cerebral palsy and others, understanding the TLRs involvement in these conditions will allow therapeutic interventions at the receptor level for treatment of these disorders.
The TLR are highly conserved protein and share structural and functional domains across species. These receptors recognize pathogen associated molecular patters (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines needed for production of immediate immunity. The cell-surface TLRs, including TLR1, TLR2, TLR4, and TLR6, recognize microbial membrane lipids, whereas TLR3, TLR7, TLR8, and TLR9 recognize pathogen-derived nucleotides in intracellular compartments. TLR7 and TLR9 respond to host-derived nucleotides as well, and they have been implicated in a variety of autoimmune diseases. Toll-like receptor 9 (TLR9) is a receptor for unmethylated CpG dinucleotides found in bacterial and viral DNA. The expression of TLR4 and TLR5 are reduced in asthmatic patients which may be partly responsible for immunopathological mechanism of asthma by reducing the release of Th1 anti-inflammatory cytokines (4). TLR7 is a membrane bound protein with two TMD. The first human toll like receptor to be identified, TLR4, senses lipopolysaccharide (LPS) while TLR2 on the other hand senses diacylated or triacylated lipopeptides after heterodimerizing with either TLR6 or TLR1, respectively. The conserved cytoplasmic TIR (Toll/IL-1 receptor) domains of the IL-1 and Toll-like receptors are the critical focal point for the generation of ligand-induced cytoplasmic signaling cascades. For signaling all the TLRs utilize one or more of the four known TIR-containing adaptor molecules: MyD88, TIRAP/MAL, TRIF, and TRAM. Human TLR9 receptor is expressed as A and B isoforms with apparent MW 125kDa (1032 aa) and 120kDa (975) with several splice variants. TLR antibody will label both A and B isoforms. TLR9 is a trans-membrane receptor protein characterized by extracellular domains with leucine-rich repeats and a cytoplasmic domain with type I IL-1 receptor. The TLR9 is expressed on cell surface of peripheral blood mononuclear cells along with major histocaompatibility comples (MHC) class II CD19 positive cells. TLR9 receptor interacts with ligand CpG ODN.
Many specific adapter molecules (TICAM, MyD88, ICAM2, TRAM, TIRAP, TRIF) are involved in signaling of several other TLRs.

General readings

General / background references:Ahmad-Nejad P et al. Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 32:1958-68 (2002). PubMed PMID: 12115616

  1. Quick-Filters:
  2. Cited (3)
  3. Highly Validated (1)

Items 1 to 20 of 56 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD289 / TLR9 antibody

CD289 / TLR9 Mouse IgG2a 5G5 Purified Can, Hu, Ms C, F, P, WB
0.25 mg / €500.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

CD289 / TLR9 Mouse IgG2a 5G5 Purified Can, Hu, Ms C, F, P, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

CD289 / TLR9 Mouse IgG2a 5G5 Purified Can, Hu, Ms C, F, P, WB
25 µg / €210.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

Immunohistochemistry of Formalin-Fixed, Paraffin-Embedded Human Spleen tissue using TLR9 Antibody Cat.-No AM33402PU-N (Clone 72-1665) at 20 µg/ml after heat-induced antigen retrieval. Rat IgG2a 72-1665 Aff - Purified Hu F, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD289 / TLR9 (C-term) antibody

Western blot analysis of TLR9 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD289 / TLR9 (Center) antibody

Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD289 / TLR9 antibody

HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC alone (thin line) or with 1 ug of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line). Rat eB72-1665 Purified Hu F, IP, P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD289 / TLR9 antibody

CD289 / TLR9 Rat M9.D6 Purified Ms F, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD289 / TLR9 antibody

WB Suggested Anti-TLR9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: ACHN cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD289 / TLR9 antibody

Western Blot: TLR9 Antibody [TA336729] - Western Blot on Jurkat whole cell lysate. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD289 / TLR9 antibody

Western Blot: TLR9 Antibody (26C593.2) [TA337047] - analysis of TLR9 antibody in A) human PBMCs, B) human intestine, C) mouse intestine and D) rat intestine tissue lysates using this antibody at 3.0 ug/ml Mouse IgG1 26C593.2 Purified Can, Eq, Hu, Ms, Prim, Rt F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD289 / TLR9 antibody

Western Blot: TLR9 Antibody (2E5) [TA337198] - TLR9 Antibody (2E5) [TA337198] - WB analysis of partial recombinant TLR9 protein (expected molecular weight ~21kDa) with TLR9 antibody (clone 2A4C6.2E5) at a concentration of 2 ug/ml. Rat IgG2a 2A4C6.2E5 Purified Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD289 / TLR9 (1-815) antibody

THP1 cells were incubated with IgG2a isptype controle (A) or α-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5 Mouse IgG2a 5G5 Biotin Can, Hu, Ms C, E, ICC/IF, P, WB
50 µg / €290.00
  Acris Antibodies GmbH

CD289 / TLR9 (1-815) antibody

THP1 cells were incubated with IgG2a isptype controle (A) or α-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5 Mouse IgG2a 5G5 FITC Can, Hu, Ms C, E, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

CD289 / TLR9 Rabbit IgG Purified Hu, Ms C, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

Figure 1. Staining TLR9 in Lung by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD289 / TLR9 antibody

Figure 1. Immunohistochemistry (Formalin-Fixed Paraffin-Embedded Tissue) - TLR9 Antibody: Staining TLR9 in Spleen by Immunohistochemistry. Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD289 / TLR9 control peptide

CD289 / TLR9
0.1 mg / €250.00
  Acris Antibodies GmbH

CD289 / TLR9 (437-455) antibody

CD289 / TLR9 Rabbit IgG Aff - Purified Chimp, Hu, Mky Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Items 1 to 20 of 56 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn