CD3 antibody

Principal name

CD3 antibody

Alternative names for CD3 antibody

T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

SwissProt ID

P07766 (Human), P22646 (Mouse), P27597 (Canfa), P29328 (Sheep), Q28073 (Bovin), Q5R1Q1 (Felca), Q7YRN2 (Pig), Q95LI5 (Macfa), Q98910 (Chick), Q9TUF9 (Rabit)

Gene ID

12501 (Cd3e), 916 (CD3E), 442981, 397455, 493936

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine), Eq (Equine), Fe (Feline), Prim (Primate), Chk (Chicken), Por (Porcine), Rhesus Mky (Rhesus Monkey), Bov (Bovine), Mky (Monkey), Rb (Rabbit), Rt (Rat), Chimp (Chimpanzee), Frog, GP (Guinea Pig), Hst (Hamster), Macaque, Op (Opossum), Orang-Utan, Sh (Sheep)

Available hosts

Hamster, Mouse, Rabbit, Rat, Goat

Available applications

Functional assay (FN), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Dot blot (Dot), Radioimmunoassay (R)

Background of CD3 antibody

The antigen receptor on human T lymphocytes consists of two variable immunoglobulin-like glycoproteins, alpha and beta or TCR-gama and delta, which occur in association (noncovalently) with three invariable T3 membrane proteins (CD3-gamma, CD3-delta and CD3 epsilon). In humans two of these proteins, T3-gamma and T3-delta, are glycoproteins of relative molecular mass (Mr) 25,000 (25K) and 20,000 (20K), respectively, while the third, T3-epsilon, is a 20K non-glycosylated protein. A keystone of the antigen-specific immune response is the T-cell receptor (TCR)/CD3 complex. CD3 antigen has gamma, delta, epsilon, and theta chains. The CD3 complex consist of various chains (alfa, beta, gamma, theta, epsilon and delta) mediates signal transduction in T cell receptor cells. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The infection of CD3 positive cells with HTLV led to a decrease in CD3 antigen expression. The onset of this phenomenon coincided with a decrease of CD3γ followed by the subsequent progressive reduction in CD3δ, then CD3ε and CD3ζ mRNA. This decrease in CD3 expression is a direct result of epigenetic control regulation of CD3 genes as determined by DNAse I hyper sensitivity assay (DHS) (1).
Antibodies to CD3 epsilon chain is a glycosylated protein of 290 amino acids with a single trans-membrane spanning domain residing between 127-149 with an apparent MW of 20 kDa. The epsilon chain of CD3 complex is non-glycosylated and is involved in rapid turnover of phosphotidylserine and accumulation of intracellular Ca after antigen receptor activation on T cell. The CD3 epsilon gene consist of nine exons and three mini exons of 21, 15 and 18 bases, the epsilon gene is transcribed form weak, tissue non-specific TATA less promoter (2).
The CD3E-selective (CD3 epsilon chain) antibodies were generated against peptide form unique region of the CD3 gene that is not present in other CD3 antigen chains or other cellular proteins. The antigenic sequence was common in several species but distinct from other CD antigens.

General readings

1. Beverley, P.C.L. and Callard, R.E. (1981) Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti T-cell antibody. Eur. J. Immunol. 11: 329-334.
2. Zarkesh-Esfahani H, Pockley G, Metcalfe RA, Bidlingmaier M, Wu Z, Ajami A, et al. High-dose leptin activates human leukocytes via receptor expression on monocytes. J Immunol. 2001 Oct 15;167(8):4593-9. PubMed PMID: 11591788.
3. Kung, P. et al. (1979) Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 206: 347-9.
4. Clevers, H. et al. (1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. Eur J Immunol. 18 (5): 705-10.
5. Salmerón, A. et al. (1991) A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 147: 3047-52.
6. Hirsh, M.I. and Cohen, V. (2006) Chloroquine prevents T lymphocyte suppression induced by anthrax lethal toxin. J Infect Dis. 194: 1003-7.
7. Mahon, N.G. et al. (2002) Immunohistologic evidence of myocardial disease in apparently healthy relatives of patients with dilated cardiomyopathy. J Am Coll Cardiol. 39: 455-62.
8. Lawson, C.A. et al. (2006) Early rheumatoid arthritis is associated with a deficit in the CD4+CD25high regulatory T cell population in peripheral blood. Rheumatology (Oxford). 45: 1210-7.
9. Campana, D. et al. (1987) The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 138 (2): 648-55.
10. Battaglia, A. et al. (2003) Lymphocyte populations in human lymph nodes. Alterations in CD4+ CD25+ T regulatory cell phenotype and T-cell receptor Vbeta repertoire. Immunology. 110: 304-12.
11. McIntosh, R.S. et al. (1997) Analysis of the T cell receptor V alpha repertoire in Hashimoto'sthyroiditis: evidence for the restricted accumulation of CD8+ T cells in the absence of CD4+ T cell restriction. J Clin Endocrinol Metab. 82: 1140-6.
12. Aird, I.A. et al. (1999) Leukocytes in semen from men with spinal cord injuries. Fertil Steril. 72: 97-103.
13. Coli?, M. et al. (2006) Correlation between phenotypic characteristics of mononuclear cells isolated from human periapical lesions and their in vitro production of Th1 and Th2 cytokines. Arch Oral Biol. 51: 1120-30.
14. Oliver, B. et al. (2001) Low-dose theophylline does not exert its anti-inflammatory effects in mild asthma through upregulation of interleukin-10 in alveolar macrophages. Allergy. 56: 1087-90.
15. Guntermann, C. and Alexander, D.R. (2002) CTLA-4 suppresses proximal TCR signaling in resting human CD4(+) T cells by inhibiting ZAP-70 Tyr(319) phosphorylation: a potential role for tyrosine phosphatases. J Immunol. 168: 4420-9.
16. Baturcam, E. et al. (2014) Physical exercise reduces the expression of RANTES and its CCR5 receptor in the adipose tissue of obese humans. Mediators Inflamm. 2014: 627150.
17. Clark, E.A. et al. (1983) Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens. Immunogenetics. 18 (6): 599-615.
18. Erber, W.N. et al. (1984) Immunocytochemical detection of T and B cell populations in routine blood smears. Lancet. 1 (8385): 1042-6.
19. Maggiorella, M. et al. (1998) Detection of Infectious Simian Immunodeficiency Virus in Band T- cell lymphomas of experimentally infected macaques. Blood. 91 (9): 3103 - 3111.
20. Kumpel, B. et al. (2014) Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis. Transfusion. 54 (5): 1305-16.
21. Mjösberg, J. et al. (2009) Systemic reduction of functionally suppressive CD4dimCD25highFoxp3+ Tregs in human second trimester pregnancy is induced by progesterone and 17beta-estradiol. J Immunol. 183: 759-69.
22. Hess, C. et al. (2000) Induction of neutrophil responsiveness to myeloperoxidase antibodies by their exposure to supernatant of degranulated autologous neutrophils. Blood. 96: 2822-7.
23. Choudhuri, K. et al. (2009) Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem. 284: 26096-105.
24. Dong, D. et al. (2006) T cell receptor for antigen induces linker for activation of T cell-dependent activation of a negative signaling complex involving Dok-2, SHIP-1, and Grb-2. J Exp Med. 203: 2509-18.
25. Libri, V. et al. (2008) Jakmip1 is expressed upon T cell differentiation and has an inhibitory function in cytotoxic T lymphocytes. J Immunol. 181: 5847-56.
26. Churchman, S.M. et al. (2014) Modulation of peripheral T-cell function by interleukin-7 in rheumatoid arthritis. Arthritis Res Ther. 16 (6): 511.
27. Ward, S.T. et al. (2015) The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Br J Cancer. 112 (2): 319-28.
28. Saavedra, D. et al. (2015) Biomarkers related to immunosenescence: relationships with therapy and survival in lung cancer patients. Cancer Immunol Immunother. Nov 20. [Epub ahead of print]
30. Bhat, S.S. et al. (2016) Syntaxin 8 is required for efficient lytic granule trafficking in cytotoxic T lymphocytes. Biochim Biophys Acta. Apr 17. pii: S0167-4889(16)30103-3. [Epub ahead of print]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD3 antibody

CD3 Mouse IgG1 PPT3 Biotin Por F
0.5 mg / €420.00
  Acris Antibodies GmbH

CD3 antibody

Human tonsil stained with CD3 Antibody Cat.-No  AM11102PU (Clone SP7) Rabbit SP7 Supernatant Bov, Can, Hu, Ms, Por, Rb, Rt F, P, WB
0.5 ml / €470.00
  Acris Antibodies GmbH

CD3 antibody

Human tonsil stained with CD3 Antibody Cat.-No  AM11102PU (Clone SP7) Rabbit SP7 Supernatant Bov, Can, Hu, Ms, Por, Rb, Rt F, P, WB
1 ml / €690.00
  Acris Antibodies GmbH

CD3 antibody

Human tonsil stained with CD3 Antibody Cat.-No  AM11102PU (Clone SP7) Rabbit SP7 Supernatant Bov, Can, Fe, Hu, Ms, Rb, Rt F, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

CD3 antibody

Formalin fixed paraffin embedded human tonsil stained with CD3 T Cell antibody DM112/DM112-05 Mouse IgG2a PS1 Supernatant Hu P
1 ml / €600.00
  Acris Antibodies GmbH

CD3 (Cytopl. Dom.) antibody

Staining of human peripheral blood lymphocytes with Rat Anti Human CD3 (SM1754F) following permeabilisation with Leucoperm. Rat IgG1 CD3-12 FITC Bov, Can, Chk, Eq, Hu, Mky, Por
0.1 mg / €510.00
  Acris Antibodies GmbH

CD3 (Cytopl. Dom.) antibody


Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody Cat.-No SM1754P following heat mediated antigen retrieval in EDTA buffer pH 9.0
Rat IgG1 CD3-12 Purified Can, Chk, Eq, Hu, Ms, Por C, F, ICC/IF, P, WB
0.2 mg / €640.00
  Acris Antibodies GmbH

CD3 (Cytopl. Dom.) antibody


Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody Cat.-No SM1754P following heat mediated antigen retrieval in EDTA buffer pH 9.0
Rat IgG1 CD3-12 Purified Can, Chk, Eq, Hu, Ms, Por C, F, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD3 antibody

CD3 Mouse IgM MEM-92 Purified Hu F, FN, IP
0.1 mg / €220.00
  Acris Antibodies GmbH

CD3 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB54 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD3 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB54 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD3 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506064) Mouse IgG2b OTIE10 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
0.1 ml / €280.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
0.2 ml / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
0.5 ml / €460.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
1 ml / €720.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 antibody

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162). Rabbit IgG SP162 Purified Hu F, P, WB
0.5 ml / €470.00
  Acris Antibodies GmbH

CD3 antibody

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162). Rabbit IgG SP162 Purified Hu F, P, WB
1 ml / €690.00
  Acris Antibodies GmbH

CD3 antibody

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162). Rabbit IgG SP162 Purified Hu F, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD3 (156-168) antibody

Human tonsil stained with anti-CD3 antibody Cat.-No AP15431PU Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

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