CD3 antibody

Principal name

CD3 antibody

Alternative names for CD3 antibody

T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

SwissProt ID

P07766 (Human), P22646 (Mouse), P27597 (Canfa), P29328 (Sheep), Q28073 (Bovin), Q5R1Q1 (Felca), Q7YRN2 (Pig), Q95LI5 (Macfa), Q98910 (Chick), Q9TUF9 (Rabit)

Gene ID

12501 (Cd3e), 916 (CD3E), 442981, 397455, 493936

Available reactivities

Ms (Mouse), Hu (Human), Can (Canine), Eq (Equine), Fe (Feline), Chk (Chicken), Rt (Rat), Prim (Primate), Por (Porcine), Rhesus Mky (Rhesus Monkey), Chimp (Chimpanzee), Mky (Monkey), Bov (Bovine), Rb (Rabbit), Frog, GP (Guinea Pig), Hst (Hamster), Macaque, Op (Opossum), Orang-Utan, Sh (Sheep), Duck

Available hosts

Hamster, Mouse, Rabbit, Rat, Goat

Available applications

Functional assay (FN), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Radioimmunoassay (R), Dot blot (Dot)

Selected product citations ‐ of AM11102PU-S

1. Kristina Scheibe, Ingo Backert, Stefan Wirtz, Axel Hueber, Georg Schett, Michael Vieth, Hans Christian Probst, Tobias Bopp, Markus F Neurath, Clemens Neufert. IL-36R signalling activates intestinal epithelial cells and fibroblasts and promotes mucosal healing in vivo. Gut doi:10.1136/gutjnl-2015-310374. PubMed PMID: 26783184.

Background of CD3 antibody

The antigen receptor on human T lymphocytes consists of two variable immunoglobulin-like glycoproteins, alpha and beta or TCR-gama and delta, which occur in association (noncovalently) with three invariable T3 membrane proteins (CD3-gamma, CD3-delta and CD3 epsilon). In humans two of these proteins, T3-gamma and T3-delta, are glycoproteins of relative molecular mass (Mr) 25,000 (25K) and 20,000 (20K), respectively, while the third, T3-epsilon, is a 20K non-glycosylated protein. A keystone of the antigen-specific immune response is the T-cell receptor (TCR)/CD3 complex. CD3 antigen has gamma, delta, epsilon, and theta chains. The CD3 complex consist of various chains (alfa, beta, gamma, theta, epsilon and delta) mediates signal transduction in T cell receptor cells. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The infection of CD3 positive cells with HTLV led to a decrease in CD3 antigen expression. The onset of this phenomenon coincided with a decrease of CD3γ followed by the subsequent progressive reduction in CD3δ, then CD3ε and CD3ζ mRNA. This decrease in CD3 expression is a direct result of epigenetic control regulation of CD3 genes as determined by DNAse I hyper sensitivity assay (DHS) (1).
Antibodies to CD3 epsilon chain is a glycosylated protein of 290 amino acids with a single trans-membrane spanning domain residing between 127-149 with an apparent MW of 20 kDa. The epsilon chain of CD3 complex is non-glycosylated and is involved in rapid turnover of phosphotidylserine and accumulation of intracellular Ca after antigen receptor activation on T cell. The CD3 epsilon gene consist of nine exons and three mini exons of 21, 15 and 18 bases, the epsilon gene is transcribed form weak, tissue non-specific TATA less promoter (2).
The CD3E-selective (CD3 epsilon chain) antibodies were generated against peptide form unique region of the CD3 gene that is not present in other CD3 antigen chains or other cellular proteins. The antigenic sequence was common in several species but distinct from other CD antigens.

General readings

1. ang H, Oura CA, Kirkham PA, Parkhouse RM. Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes. Immunology. 1996;88:577-85. 
2. Jamin A, Gorin S, Le Potier M, Kuntz-Simon G. Characterization of conventional and plasmacytoid dendritic cells in swine secondary lymphoid organs and blood. Vet Immunol Immunopathol. 2006;114:224-37. 
3. Kvist PH, Iburg T, Dawson HD, Jensen HE. Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs. Diabetes Technol Ther. 2010;12:791-9. 
4. Debeer S, Le Luduec J, Kaiserlian D, Laurent P, Nicolas J, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dematol. 2013;23:456-66.
5. Tingstedt J, Nielsen J. Cellular immune responses in the lungs of pigs infected in utero with PRRSV: an immunohistochemical study. Viral Immunol. 2004;17:558-64.
6. Jung K, Alekseev KP, Zhang X, Cheon D, Vlasova AN, Saif LJ. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol. 2007;81:13681-93.
7. Isling LK, Aalbæk B, Schrøder M, Leifsson PS. Pyelonephritis in slaughter pigs and sows: morphological characterization and aspects of pathogenesis and aetiology. Acta Vet Scand. 2010;52:48. 
8. Toka FN, Nfon CK, Dawson H, Golde WT. Accessory-cell-mediated activation of porcine NK cells by toll-like receptor 7 (TLR7) and TLR8 agonists. Clin Vaccine Immunol. 2009;16:866-78. 
9. Toka FN, Nfon CK, Dawson H, Estes DM, Golde WT. Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells. J Interferon Cytokine Res. 2009;29:179-92.
10. Toka FN, Nfon C, Dawson H, Golde WT. Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus. Clin Vaccine Immunol. 2009;16:1738-49. 
11. Wen K, Bui T, Li G, Liu F, Li Y, Kocher J, et al. Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection. Comp Immunol Microbiol Infect Dis. 2012;35:289-301. 
12. Liu KY, Comstock SS, Shunk JM, Monaco MH, Donovan SM. Natural killer cell populations and cytotoxic activity in pigs fed mother's milk, formula, or formula supplemented with bovine lactoferrin. Pediatr Res. 2013;74:402-7. 
13. Lohse L, Nielsen J, Eriksen L. Temporary CD8+ T-cell depletion in pigs does not exacerbate infection with porcine reproductive and respiratory syndrome virus (PRRSV). Viral Immunol. 2004;17:594-603. 
14. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. 
15. Yu S, Vincent A, Opriessnig T, Carpenter S, Kitikoon P, Halbur PG, et al. Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro. Vet Microbiol. 2007;123:34-42. 
16. Shi K, Li H, Guo X, Ge X, Jia H, Zheng S, et al. Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet Microbiol. 2008;129:367-77. 
17. Yuan L, Wen K, Azevedo MS, Gonzalez AM, Zhang W, Saif LJ. Virus-specific intestinal IFN-γ producing T cell responses induced by human rotavirus infection and vaccines are correlated with protection against rotavirus diarrhea in gnotobiotic pigs. Vaccine. 2008;26:3322-31.
18. Facci MR, Auray G, Buchanan R, van Kessel J, Thompson DR, Mackenzie-Dyck S, et al. A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs. Immunology. 2010;129:396-405.
19. Martelli P, Gozio S, Ferrari L, Rosina S, De Angelis E, Quintavalla C, et al. Efficacy of a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs naturally exposed to a heterologous European (Italian cluster) field strain: Clinical protection and cell-mediated immunity. Vaccine. 2009;27:3788-99. 
20. Diaz-San Segundo F, Moraes MP, de Los Santos T, Dias CC, Grubman MJ. Interferon-induced protection against foot-and-mouth disease virus infection correlates with enhanced tissue-specific innate immune cell infiltration and interferon-stimulated gene expression. J Virol. 2010;84:2063-77.
21. Ferrari L, Borghetti P, Gozio S, De Angelis E, Ballotta L, Smeets J, et al. Evaluation of the immune response induced by intradermal vaccination by using a needle-less system in comparison with the intramuscular route in conventional pigs. Res Vet Sci. 2011;90:64-71.
22. Oh Y, Seo HW, Han K, Park C, Chae C. Protective effect of the maternally derived porcine circovirus type 2 (PCV2)-specific cellular immune response in piglets by dam vaccination against PCV2 challenge. J Gen Virol. 2012;93:1556-62.
23. Chattha KS, Vlasova AN, Kandasamy S, Rajashekara G, Saif LJ. Divergent immunomodulating effects of probiotics on T cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model. J Immunol. 2013;191:2446-56.
24. Liu P, Pieper R, Tedin L, Martin L, Meyer W, Rieger J, et al. Effect of dietary zinc oxide on jejunal morphological and immunological characteristics in weaned piglets. J Anim S. 2014;92:5009-18.
25. Pasternak JA, Ng SH, Käser T, Meurens F, Wilson HL. Grouping pig-specific responses to mitogen with similar responder animals may facilitate the interpretation of results obtained in an out-bred animal model. J Vaccines Vaccin. 2014;5:5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD3 antibody

CD3 Mouse IgG1 PPT3 Biotin Por F
0.5 mg / €420.00
  Acris Antibodies GmbH

CD3 antibody

Human tonsil stained with CD3 Antibody Cat.-No  AM11102PU (Clone SP7) Rabbit SP7 Supernatant Bov, Can, Hu, Ms, Por, Rb, Rt F, P, WB
0.5 ml / €460.00
  Acris Antibodies GmbH

CD3 antibody

Human tonsil stained with CD3 Antibody Cat.-No  AM11102PU (Clone SP7) Rabbit SP7 Supernatant Bov, Can, Hu, Ms, Por, Rb, Rt F, P, WB
1 ml / €680.00
  Acris Antibodies GmbH

CD3 antibody

Human tonsil stained with CD3 Antibody Cat.-No  AM11102PU (Clone SP7) Rabbit SP7 Supernatant Bov, Can, Fe, Hu, Ms, Rb, Rt F, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

CD3 antibody

Formalin fixed paraffin embedded human tonsil stained with CD3 T Cell antibody DM112/DM112-05 Mouse IgG2a PS1 Supernatant Hu P
1 ml / €590.00
  Acris Antibodies GmbH

CD3 (Cytopl. Dom.) antibody

Staining of human peripheral blood lymphocytes with Rat Anti Human CD3 (SM1754F) following permeabilisation with Leucoperm. Rat IgG1 CD3-12 FITC Bov, Can, Chk, Eq, Hu, Mky, Por
0.1 mg / €510.00
  Acris Antibodies GmbH

CD3 (Cytopl. Dom.) antibody


Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody Cat.-No SM1754P following heat mediated antigen retrieval in EDTA buffer pH 9.0
Rat IgG1 CD3-12 Purified Can, Chk, Eq, Hu, Ms, Por C, F, ICC/IF, P, WB
0.2 mg / €640.00
  Acris Antibodies GmbH

CD3 (Cytopl. Dom.) antibody


Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody Cat.-No SM1754P following heat mediated antigen retrieval in EDTA buffer pH 9.0
Rat IgG1 CD3-12 Purified Can, Chk, Eq, Hu, Ms, Por C, F, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD3 antibody

CD3 Mouse IgM MEM-92 Purified Hu F, FN, IP
0.1 mg / €220.00
  Acris Antibodies GmbH

CD3 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB54 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD3 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG2b UMAB54 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD3 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506064) Mouse IgG2b OTIE10 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
0.1 ml / €280.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
0.2 ml / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
0.5 ml / €460.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 μm) stained with anti-CD3 antibody (DB 082) shows strong immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D. from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG N26-R Aff - Purified Hu C, P
1 ml / €720.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD3 antibody

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162). Rabbit IgG SP162 Purified Hu F, P, WB
0.5 ml / €460.00
  Acris Antibodies GmbH

CD3 antibody

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162). Rabbit IgG SP162 Purified Hu F, P, WB
1 ml / €680.00
  Acris Antibodies GmbH

CD3 antibody

Human Tonsil stained with anti-CD3 Antibody Cat.-No AM32443PU (Clone SP162). Rabbit IgG SP162 Purified Hu F, P, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

CD3 (156-168) antibody

Human tonsil stained with anti-CD3 antibody Cat.-No AP15431PU Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

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