CD300E / CMRF35A5 antibody

Principal name

CD300E / CMRF35A5 antibody

Alternative names for CD300E / CMRF35A5 antibody

CLM-2, CD300LE, CLM2, IREM2, CMRF35-A5, CMRF35-like molecule 2, Immune receptor expressed on myeloid cells 2, CD300 antigen-like family member E

SwissProt ID

Q496F6 (Human), Q8K249 (Mouse)

Gene ID

342510 (CD300E)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Functional assay (FN)

Background of CD300E / CMRF35A5 antibody

The monoclonal antibody UP-H2 recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE. It is a member of the CMRF-35 family. This 34 kDa Ig-like containing receptor is an activating receptor and through its charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12. Expression is found exclusively on monocytes and myeloid DCs. Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs. It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2. In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes.

General readings

Clark G J., et al. (2009) Immunobiology. 214: 730-736.
Clark G J., et al. (2009) Trends Immunol. 30: 209-217.

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD300E / CMRF35A5 antibody

Staining of normal human peripheral blood cells with 0.06 ug of Mouse IgG1 kappa Isotype Control Purified (blue histogram) or 0.06 ug of Anti-Human CD300e (IREM-2) Purified (purple histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Cells in the monocyte gate were used for analysis. Mouse UP-H2 Purified Hu F, IP, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD300E / CMRF35A5 antibody

CD300E / CMRF35A5 Mouse IgG1 UP-H2 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD300E / CMRF35A5 antibody

CD300E / CMRF35A5 Mouse IgG1 UP-H2 Purified Hu F, FN, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD300E / CMRF35A5 antibody

CD300E / CMRF35A5 Mouse IgG1 UP-H2 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD300E / CMRF35A5 antibody

Western Blot analysis of CD300E expression in transfected 293T cell line (H00342510-T01) by CD300E MaxPab polyclonal antibody.

Lane 1: CD300LE transfected lysate(22.55 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CD300E / CMRF35A5 antibody

Flow Cytometry using human peripheral blood cells ( 1 µg/106 cells ).
FACSCalibur ( Becton Dickinson, San Jose, CA ) flow cytometer, using Cell Quest acquisition software and PAINT-A-GATE. PRO, analysis software. Mouse IgG2a UP-H2 Hu F
100 reaction / €640.00
  Abnova Taiwan Corp.

CD300E / CMRF35A5 antibody

CD300E / CMRF35A5 Mouse IgG1 UP-H2 Aff - Purified Hu F, IP, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

CD300E / CMRF35A5 antibody

CD300E / CMRF35A5 Mouse IgG1 UP-H2 Aff - Purified Hu F, IP, WB
25 µg / €310.00
  Novus Biologicals Inc.

CD300E / CMRF35A5 antibody

Immunohistochemistry-Paraffin: CD300E Antibody [NBP1-84592] Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD300E / CMRF35A5 (18-173, His-tag)

CD300E / CMRF35A5 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

CD300E / CMRF35A5 (18-173, His-tag)

CD300E / CMRF35A5 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

CD300E / CMRF35A5

CD300E / CMRF35A5 Human
10 µg / €540.00
  Abnova Taiwan Corp.

CD300E / CMRF35A5

CD300E / CMRF35A5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

CD300E / CMRF35A5

CD300E / CMRF35A5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CD300E 293T Cell Transient Overexpression Lysate(Denatured)

CD300E 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

15 Item(s)

per page
  • LinkedIn