CD309 / VEGFR-2 / Flk-1 antibody

Principal name

CD309 / VEGFR-2 / Flk-1 antibody

Alternative names for CD309 / VEGFR-2 / Flk-1 antibody

VEGFR2, FLK1, KDR, VEGF Receptor 2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor Flk-1, Kinase insert domain receptor, Kinase NYK

SwissProt ID

O08775 (Rat), P35918 (Mouse), P35968 (Human)

Gene ID

3791 (KDR), 16542

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Bov (Bovine), Chk (Chicken), Prim (Primate), Ze (Zebrafish), Fe (Feline), Mky (Monkey), Hst (Hamster)

Available hosts

Rabbit, Mouse, Goat, Chicken, Rat

Available applications

Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F), Functional assay (FN), Neutralisation (NEUT)

Background of CD309 / VEGFR-2 / Flk-1 antibody

Vascular endothelial growth factor (VEGF), also called vasculotropin (VAS) and vascular permeability factor (VPF), is a member of a family of endothelial cell mitogens and angiogenic factors. VEGF is a homodimeric heparin-binding glycoprotein, which specifically stimulates the proliferation of endothelial cells isolated from both small and large vessels. These include endothelial cells from adrenal cortex, cerebral cortex, fetal and adult aorta and human umbilical vein. The mitogenic activity of VEGF appears to be stimulated by specific VEGF receptors (160-200 kDa) which can be found on the surface of various endothelial cells. VEGF binds to two structurally similar receptor tyrosine kinases; Flt17(fms-like tyrosine kinase 1, also known as VEGF Receptor 1), and KDR8 (kinase-insert domain containing receptor, also known as VEGF Receptor 2). The homologous gene for the human KDR gene is mouse Flk1 (fetal liver kinase 1) and rat TKrc Both receptors are members of a superfamily of receptor tyrosine kinases (RTKs) which include PDGF receptor, c-fms, M-CSF receptor and c-kit - the receptor for stem-cell factor. The KDR gene has been mapped to human chromosome 4q11-q12, which is the same locus of PDGF receptor and c-kit. The extracellular domains of KDR/Flk1 and Flt1 (approx. 90 kDa), have seven immunoglobulin-like domains and belong to the class III RTKs. This family includes also Flt4, which shares a high homology with the two VEGF receptors. Studies using KDR and Flt1 stably transfected endothelial cell lines have shown that these two receptors exhibit different affinities to VEGF and mediate different responses. KDR expressing cells show striking changes in cell morphology, actin reorganization and membrane ruffling, chemotaxis and mitogenicity in response to VEGF, while Flt1 expressing cells lack such responses. Both KDR and Flt1 are phosphorylated in response to VEGF, however KDR much more efficiently. KDR/Flk1 does not respond to placental growth factor (PlGF), a VEGF related growth factor, while Flt1 binds PlGF specifically. The expression pattern of the two receptors is somewhat different; Flt1 is predominately expressed in human placenta and human vascular endothelial cells, while KDR is more widely expressed in all vessel-derived endothelial cells but low in human and fetal bovine placenta. Both VEGF receptors (KDR and Flt1) are upregulated in human fetal and in adult kidney. It has been suggested that in human postnatal hematopoietic tissues, 0.1% to 0.5% of CD34+ cells express VEGF Receptor 2/KDR; pluripotent hematopoietic stem cells (HSCs) were found to be restricted to the CD34+KDR+ cell fraction, while lineage-committed hematopoietic progenitor cells (HPCs) were found in the CD34+KDR- subset. Antibodies that react specifically with VEGF receptors are useful for the study of the specific differential tissue expression and intracellular localization of the receptor in normal and neoplastic tissue.

General readings

Western Blot Protocol
1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a cell lysate sample (cell lysis buffer: 50 mM Tris-HCl, pH 7.4; 1% NP-40; 0.25% sodium deoxycholate; 150 mM NaCl; 1 mM EDTA; 1 mM PMSF; 1 μg/mL each aprotinin, leupeptin, pepstatin; 1 mM Na3VO4; 1 mM NaF) and transfer the proteins to PVDF (Immobilon-P). Wash the blotted PVDF (Immobilon-P) twice with Tris/0.05% Tween.
2. Block the blotted PVDF in freshly prepared TBST containing 5% nonfat dry milk (Catalog # 20-200), for 0.5 hour at room temperature with constant agitation.
3. Incubate the PVDF with 1mg/mL (1/1,000) of Anti-phospho-VEGFR2 (Tyr1054), diluted in freshly prepared TBST-MILK at room temperature for 2 hours at room temperature (or alternately overnight at 4°C) with agitation
4. Wash the PVDF 4 times with TBST for 3-5 minutes each.
5. Incubate the PVDF in the secondary reagent of choice (donkey anti-Rabbit- HRP conjugate in TBST-MILK for 1 hour at room temperature with agitation.
7. Wash the PVDF 4 times in TBST for 3-5 minutes each wash.
9. Use detection method of choice (enhanced chemiluminescence was used).

  1. Quick-Filters:
  2. Highly Validated (11)
  3. Overnight Delivery (2)

Items 1 to 20 of 188 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD309 / VEGFR-2 / Flk-1 antibody

Western Blot: VEGF Receptor 2 Antibody (1B6) [TA337222] - Western blot analysis of VEGF Receptor 2 (1B6) in purified human recombinant protein Mouse IgG1 1B6 Purified Hu C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

Western Blot: VEGF Receptor 2 Antibody (2C6) [TA337223] - Western blot analysis of VEGFR2 (clone 2C6) in partial recombinant protein Mouse IgG1 2C6 Purified Hu F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 (N-term) antibody

Human Prostate Adenocarcinoma stained with FLK1 Antibody Cat.-No AM21042PU (Clone SP123) Rabbit IgG SP123 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 (N-term) antibody

Human Prostate Adenocarcinoma stained with FLK1 Antibody Cat.-No AM21042PU (Clone SP123) Rabbit IgG SP123 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 (N-term) antibody

Human Prostate Adenocarcinoma stained with FLK1 Antibody Cat.-No AM21042PU (Clone SP123) Rabbit IgG SP123 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunofluorescence staining of VEGFR-2/Flk-1 in mouse primary endothelial cells (SnoMec) with monoclonal rat anti-mouse VEGFR-2/Flk-1 Cat.-No AM33395PU-N (Clone 3C8) (upper panel).Control with goat anti-rat ALEXA Flour 488 (lower panel). Rat IgG1 3C8 Purified Ms F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Western Analysis of anti-Human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Mouse IgG1 KDREIC Purified Hu C, E, F, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Figure 5. Consecutive sections of unfixed, human foreskin. A) Staining with anti-soluble VEGFR2/KDR antibodies (Cat.-No AP26034PU-L). Note signal in epidermis and vessels. B) Staining with anti-membrane-bound VEGFR-2/KDR (Cat.-No DM3522P). Note staining in vessels. C) Negative control. Note non-specific fluorescence in the hornified layer of the epithelium. Provided by Prof. J. Wilting, Göttingen, Germany. Mouse IgG1 4H3 Purified Hu E, F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Up-regulation of VEGFR-2 in vein ECs of an intact Human umbilical cord by bFGF: A fresh human umbilical cord was rinsed with PBS to remove residual blood cells, cut in small pieces (about 0.5 cm), incubated in EBM (1% FCS) and stimulated with or without 20 ng/ml bFGF for 24 h. Pieces were frozen in liquid nitrogen and used for immunohistochemistry using the mab anti-human VEGFR-2 antibody Cat.-No DM3523P as detection antibody. (Bernhard Barleon et al., unpublished data!) Mouse IgG1 4 (20I6) Purified Hu C, E, F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunohistochemistry with Cryo sections using polyclonal anti-Human VEGFR-2/KDR antibody (RT, Cat.-No DP3509S). The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany. Rabbit Azide Free Hu C, E, FN, IP, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunohistochemistry with Cryo sections using polyclonal anti-Human VEGFR-2/KDR antibody (RT, Cat.-No DP3509S). The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany. Rabbit Azide Free Hu C, E, FN, IP, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Human placenta stained with Anti-Flk-1 antibody Rabbit Purified Hu, Ms P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Human placenta stained with Anti-Flk-1 antibody Rabbit Purified Hu, Ms P
1 ml / €440.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Human placenta stained with Anti-Flk-1 antibody Rabbit Purified Hu, Ms P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunofluorescence analysis of MCF-7 cells, using GPR42 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

Surface staining of bEnd.3 cell line with Anti-Mouse CD309 (FLK1) PE.  Appropriate isotype controls were used  (open histogram).  Total viable cells were used for analysis. Rat Avas12a1 Purified Ms F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

Predicted band size: 152 kDa . Positive control: Rat brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

CD309 / VEGFR-2 / Flk-1 Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

Western blot analysis of extracts of HT29 cell line, using VEGFR2 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

Western Blot: VEGF Receptor 2 Antibody [TA336288] - Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using TA336288. ECL 10 second exposure. Rabbit Purified Hst, Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Items 1 to 20 of 188 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn