CD309 / VEGFR-2 / Flk-1 antibody

Principal name

CD309 / VEGFR-2 / Flk-1 antibody

Alternative names for CD309 / VEGFR-2 / Flk-1 antibody

VEGFR2, FLK1, KDR, VEGF Receptor 2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor Flk-1, Kinase insert domain receptor, Kinase NYK

SwissProt ID

O08775 (Rat), P35918 (Mouse), P35968 (Human)

Gene ID

3791 (KDR), 16542 (Kdr)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Mky (Monkey), Hst (Hamster)

Available hosts

Rabbit, Mouse, Chicken, Rat, Goat

Available applications

Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Functional assay (FN), Immunoprecipitation (IP)

Selected product citations ‐ of AM21042PU-N

1. KÖLBL, AC;BIRK, AE;KUHN, C;JESCHKE, U;ANDERGASSEN, U. Influence of VEGFR and LHCGR on endometrial adenocarcinoma. ONCOLOGY LETTERS 2016, 2092-2098, 12. DOI: 10.3892/ol.2016.4906

Background of CD309 / VEGFR-2 / Flk-1 antibody

Disruption of the precise balance of positive and negative molecular regulators of blood and lymphatic vessel growth can lead to myriad diseases. Although dozens of natural inhibitors of hemangiogenesis have been identified, an endogenous selective inhibitor of lymphatic vessel growth has not to our knowledge been previously described. A splice variant of the gene encoding vascular endothelial growth factor receptor-2 (VEGFR-2) that encodes a secreted form of the protein, designated endogenous soluble VEGFR-2 (esVEGFR-2/KDR) has been described. The endogenous soluble esKDR inhibits developmental and reparative lymphangiogenesis by blocking VEGF-C function. Tissue-specific loss of esKDR in mice induced, at birth, spontaneous lymphatic invasion of the normally alymphatic cornea and hyperplasia of skin lymphatics without affecting blood vasculature. Administration of esKDR inhibited lymphangiogenesis but not hemangiogenesis induced by corneal suture injury or transplantation, enhanced corneal allograft survival and suppressed lymphangioma cellular proliferation. Naturally occurring esKDR thus acts as a molecular uncoupler of blood and lymphatic vessels; modulation of esKDR might have therapeutic effects in treating lymphatic vascular malformations, transplantation rejection and, potentially, tumor lymphangiogenesis and lymphedema. 
Recombinant mouse esFlk-1 generated by alternative splicing consists of the first 6 Ig-like loops followed by the unique C-terminal end: GMEASLGDRIAMP.

General readings

Simon et al., J Am Soc Nephrol 9:1032, 1998; Clauss et al., J Biol Chem 271:17629, 1996; Morbidelli et al., Angiogenesis 1:117, 1997.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD309 / VEGFR-2 / Flk-1 antibody

  Mouse Monoclonal VEGF R2/KDR/Flk-1 Antibody (1B6)  
Western Blot: VEGF Receptor 2 Antibody (1B6) [TA337222] - Western blot analysis of VEGF Receptor 2 (1B6) in purified human recombinant protein Mouse IgG1 1B6 Purified Hu C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Mouse Monoclonal VEGF R2/KDR/Flk-1 Antibody (2C6)  
Western Blot: VEGF Receptor 2 Antibody (2C6) [TA337223] - Western blot analysis of VEGFR2 (clone 2C6) in partial recombinant protein Mouse IgG1 2C6 Purified Hu F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

Immunofluorescence staining of VEGFR-2/Flk-1 in mouse primary endothelial cells (SnoMec) with monoclonal rat anti-mouse VEGFR-2/Flk-1 Cat.-No AM33395PU-N (Clone 3C8) (upper panel).Control with goat anti-rat ALEXA Flour 488 (lower panel). Rat IgG1 3C8 Purified Ms F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Western Analysis of anti-Human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Mouse IgG1 KDREIC Purified Hu C, E, F, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Figure 5. Consecutive sections of unfixed, human foreskin. A) Staining with anti-soluble VEGFR2/KDR antibodies (Cat.-No AP26034PU-L). Note signal in epidermis and vessels. B) Staining with anti-membrane-bound VEGFR-2/KDR (Cat.-No DM3522P). Note staining in vessels. C) Negative control. Note non-specific fluorescence in the hornified layer of the epithelium. Provided by Prof. J. Wilting, Göttingen, Germany. Mouse IgG1 4H3 Purified Hu E, F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Up-regulation of VEGFR-2 in vein ECs of an intact Human umbilical cord by bFGF: A fresh human umbilical cord was rinsed with PBS to remove residual blood cells, cut in small pieces (about 0.5 cm), incubated in EBM (1% FCS) and stimulated with or without 20 ng/ml bFGF for 24 h. Pieces were frozen in liquid nitrogen and used for immunohistochemistry using the mab anti-human VEGFR-2 antibody Cat.-No DM3523P as detection antibody. (Bernhard Barleon et al., unpublished data!) Mouse IgG1 4 (20I6) Purified Hu C, E, F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunohistochemistry with Cryo sections using polyclonal anti-Human VEGFR-2/KDR antibody (RT, Cat.-No DP3509S). The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany. Rabbit Azide Free Hu C, E, FN, IP, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit anti-human KDR [B, #DP3509P], (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE. Rabbit IgG Aff - Purified Hu E, F, FN, ICC/IF, IP, WB
50 µg / €270.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunohistochemistry with Cryo sections using polyclonal anti-Human VEGFR-2/KDR antibody (RT, Cat.-No DP3509S). The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany. Rabbit Azide Free Hu C, E, FN, IP, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit anti-VEGFR (Phospho-Tyr1175) polyclonal antibody (Phospho-specific)  
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (phospho-Tyr1175) antibody. Rabbit IgG Purified Hu, Ms, Rt P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit anti-VEGFR (Phospho-Tyr1214) polyclonal antibody (Phospho-specific)  
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (phospho-Tyr1214) antibody. Rabbit IgG Purified Hu, Ms, Rt P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit anti-VEGFR (Phospho-Tyr951) polyclonal antibody (Phospho-specific)  
Western blot analysis of extracts from SK-OV3 cells using VEGFR2 antibody (Lanes 1 and 2) and VEGRF2 (phospho-Tyr951) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit polyclonal KDR / VEGFR2 (Tyr1214) antibody(Phospho-specific)  
Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using VEGFR2 (Phospho-Tyr1214) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit polyclonal anti-GPR42 antibody  
Immunofluorescence analysis of MCF-7 cells, using GPR42 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rat Anti-Mouse CD309 (FLK1) Purified (100 ug)  
Surface staining of bEnd.3 cell line with Anti-Mouse CD309 (FLK1) PE.  Appropriate isotype controls were used  (open histogram).  Total viable cells were used for analysis. Rat Avas12a1 Purified Ms F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Anti-KDR Rabbit Polyclonal Antibody  
Predicted band size: 152 kDa . Positive control: Rat brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Anti-KDR (phospho-Tyr1059) Rabbit Polyclonal Antibody  
Predicted band size: 152 kDa . Positive control: HT29 cells treated with SCF-1 lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells untreated with SCF-1 lysate Lane2: HT29 cells treated with SCF-1 lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit Polyclonal VEGFR2 Antibody  
CD309 / VEGFR-2 / Flk-1 Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit Polyclonal VEGFR2 (Tyr1059) Antibody (Phospho-specific)  
Western blot analysis of VEGFR2 phosphorylation expression in Na3VO4 treated HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD309 / VEGFR-2 / Flk-1 antibody

  Rabbit Polyclonal VEGFR2 (Tyr1214) Antibody (Phospho-specific)  
Western blot analysis of VEGFR2 phosphorylation expression in Na3VO4 treated HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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