CD31 / PECAM1 antibody
Principal name
CD31 / PECAM1 antibody
Alternative names for CD31 / PECAM1 antibody
PECAM-1, EndoCAM, GPIIA', Platelet endothelial cell adhesion molecule
Ncbi ID
9606, 10090, NP_113779.1, 9913, NP_999072.1, NP_000433, 9823, NP_001027550, NP_004235, NP_000433.4, NP_001027550.1, NM_000442, NM_001032378, NM_008816, NM_031591
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Luminex (LMNX)
Selected product citations ‐ of AP15436PU-S
1. Moghadamrad S, McCoy KD, Geuking MB, Sägesser H, Kirundi J, Macpherson AJ, et al. Attenuated portal hypertension in germ-free mice: Function of bacterial flora on the development of mesenteric lymphatic and blood vessels. Hepatology. 2015 Jan 16. doi: 10.1002/hep.27698. PubMed PMID: 25643846.
2. Pfankuche, VM;Spitzbarth, I;Lapp, S;Ulrich, R;Deschl, U;Kalkuhl, A;Baumgärtner, W;Puff, C. Reduced angiogenic gene expression in morbillivirus-triggered oncolysis in a translational model for histiocytic sarcomaJ. Cell. Mol. Med. 2016. PubMed PMID: 27860224.
Background of CD31 / PECAM1 antibody
Platelet endothelial cell adhesion molecule-1 (PECAM-1) or cluster designation 31 (CD31) is a member of the immunoglobulin (Ig) gene superfamily and a transmembrane glycoprotein. The CD31 protein is widely expressed by cells of the vascular endothelium lineage, platelets, Kupffer cells, granulocytes, megakaryocytes, monocytes, neutrophils, and some types of T-cells. CD31 functions as a cell adhesion molecule in endothelial cell homotypic interactions in the process of angiogenesis. CD31 is also involved in leukocyte migration and integrin activation. It plays a key role in the adhesion cascade between the endothelial cells and inflammatory cells, enabling leukocyte migration in inflammatory sites. CD31 is one of the best markers for benign and malignant vascular tumors. It is expressed in certain tumors including angiomas and angiosarcomas including epitheloid hemangioendothelioma, epitheloid sarcoma-like hemangioendothelioma. Its expression in Kaposi sarcoma lesions is variable. Human CD31 comprises 738 amino acids and its predicted molecular weight would therefore be approximately 81kDa. However the observed molecular weight, as reported by various research groups including our own, is higher and at around 120kDa and 140kDa. The disparity probably reflects post translational modifications including glycosylation and probably because different isoforms of the protein may exist.
General readings
1. Williams KC, Zhao RW, Ueno K, Hickey WF. PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J Neurosci Res. 1996 Sep 15;45(6):747-57. PubMed PMID: 8892086.
2. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia / reperfusion injury. Am J Pathol. 163: 1587-98.
3. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 18: 1389-98.
4. Ott, I. et al. (2005) Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction. FASEB J. 19: 992-4.
5. Fujimoto, K.L. et al. (2007) An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction. J Am Coll Cardiol. 49: 2292-300.
6. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation. J Immunol. 183: 3099-108.
7. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration. J Pharmacol Exp Ther. 286:447-58.
8. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis. Arthritis Rheuma48: 2173-7.
9. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 157: 647-58.
10. Lochhead, J.J. et al. (2010) Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation. J Cereb Blood Flow Metab. 30:
1625-36.11. Arkudas, A. et al. (2007) Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model. Mol Med. 13: 480-7.
12. Nakao, A. et al. (2011) Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts. Cardiovasc Res. 89: 457-63.
13. Ohnishi, T. et al. (2007) Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans. Toxicol Pathol. 35: 904-9.
14. Schilte, M.N. et al. (2009) Long-term intervention with heparins in a rat model of peritoneal dialysis. Perit Dial Int. 29: 26-35.
15. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation. J Pharmacol Exp Ther. 306:8-12.
16. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J Immunol Methods. 322: 137-42.
17. Willis, C.L. et al. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. J Cereb Blood Flow Metab. 30: 1847-59.
18. Salehi-Had, H. et al. (2011) Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization. PLoS One. 6: e18864.
19. MacLean, A.G. et al. (2001) Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells. J Neuroimmunol. 118: 223-32.
20. Ceelen, W. et al. (2007) Recombinant human erythropoietin alpha modulates the effects of radiotherapy on colorectal cancer microvessels. Br J Cancer. 96: 692-700.
21. Tung, H.C. et al. (2015) The Beneficial Effects of P2X7 Antagonism in Rats with Bile Duct Ligation-induced Cirrhosis. PLoS One. 10 (5): e0124654.
22. Oboshi, M. et al. (2015) Temporary dietary iron restriction affects the process of thrombus resolution in a rat model of deep vein thrombosis. PLoS One. 10 (5): e0126611.
23. Wu, S.H. et al. (2015) Autologous adipose-derived stem cells attenuate muscular atrophy and protect spinal cord ventral horn motor neurons in an animal model of burn injury.Cytotherapy. 17 (8): 1066-75.
Figure 3. BM4047 CD31 antibody staining of Human Placenta Paraffin Section.
Figure 2. BM4047 CD31 antibody staining of Human Skin Frozen Section.
Figure 1. BM4047 CD31 antibody staining of Human Tonsil Frozen Section.
Immunohistochemical staining on Mouse Kidney Frozen Sections using CD31 antibody clone ER-MP12 Cat.-No BM4086B.
Immunohistochemical staining on Mouse Kidney Frozen Sections using CD31 antibody clone ER-MP12 Cat.-No BM4086B.
Immunohistochemical staining on Mouse Kidney Frozen Sections using CD31 antibody clone ER-MP12 Cat.-No BM4086B.
Surface staining of lysed and washed human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE).
Surface staining of human peripheral blood with anti-human CD31 (MEM-05) biotin.
Surface staining of lysed and washed human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE).
Surface staining of lysed and wash human peripheral blood with purified anti-Human CD31 antibody (MEM-05) (detection by Goat anti-mouse IgG1 PE).
Surface staining of human peripheral blood with anti-human CD31 (MEM-05) biotin.
Immunoperoxidase of monoclonal antibody to PECAM1 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma tissue. [antibody concentration 5ug/ml]
PECAM1 monoclonal antibody (M01), clone 1D2-1A5. Western Blot analysis of PECAM1 expression in human placenta.
Staining of sheep peripheral blood platelets with MOUSE ANTI OVINE CD31 antibody (SM464F).
Surface staining of human peripheral blood with anti-human CD31 (MEM-05) biotin.
Surface staining of lysed and washed human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE).
Surface staining of human peripheral blood with anti-human CD31 (MEM-05) biotin.
Surface staining of lysed and washed human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE).
Immunohistochemistry analysis of PECAM-1/CD31 antibody (Cat.-No AP06560PU-N) in paraffin-embedded human brain tissue.
Western blot analysis of PECAM-1/CD31 antibody (Cat.-No AP06560PU-N) at 1/500 dilution:
Lane 1: Hela whole cell lysate treated with LPS.
Lane 2: PC12 whole cell lysate.
Lane 3: sp2/0 whole cell lysate.
Lane 1: Hela whole cell lysate treated with LPS.
Lane 2: PC12 whole cell lysate.
Lane 3: sp2/0 whole cell lysate.
Staining of porcine peripheral blood lymphocytes with Mouse Anti Pig CD31-APC (SM2146APC).
Surface staining of lysed and wash human peripheral blood with purified PECAM1 monoclonal antibody, clone MEM - 05 ( Cat # MAB0913 ) ( detection by Goat anti - mouse IgG1 PE ) .
PECAM1 MaxPab polyclonal antibody. Western Blot analysis of PECAM1 expression in human placenta.
Western Blot analysis of PECAM1 expression in transfected 293T cell line (H00005175-T01) by PECAM1 MaxPab polyclonal antibody.Lane 1: PECAM1 transfected lysate(81.18 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PECAM1 and CTNNB1. HeLa cells were stained with anti-PECAM1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
PECAM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of PECAM1 expression in human placenta.
Western Blot analysis of PECAM1 expression in transfected 293T cell line (H00005175-T01) by PECAM1 MaxPab polyclonal antibody.Lane 1: PECAM1 transfected lysate(82.40 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of CD31 antibody (C-term) in HL60 cell line lysates (35ug/lane). CD31 (arrow) was detected using the purified Pab.
AP15436PU CD31 antibody staining of Human Tonsil.
Human Cervix stained with anti-CD31 antibody Cat.-No AM11103PU
Human Colon stained with anti-CD31 antibody Cat.-No AM11103PU
Human Tonsil stained with anti-CD31 antibody Cat.-No AM11103PU
Mouse Kidney stained with anti-CD31 antibody Cat.-No AM11103PU
AM08396PU-N CD31 antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 5 µg/ml followed by Biotin conjugated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
BALB/c splenocytes were first incubated with purified Pecam1 monoclonal antibody, clone 390 ( Cat # MAB3230 ) and then with goat anti-rat Ig-FITC. Small lymphocytes were gated and analyzed on a FACScanTM flow cytometer ( BDIS, San Jose, CA ).
PECAM1 MaxPab polyclonal antibody. Western Blot analysis of PECAM1 expression in human placenta.
Western Blot analysis of PECAM1 expression in transfected 293T cell line (H00005175-T01) by PECAM1 MaxPab polyclonal antibody.Lane 1: PECAM1 transfected lysate(81.18 KDa).Lane 2: Non-transfected lysate.
T-2001 CD31 (PECAM-1; macrophage precursors), rat anti mouse, clone ER-MP12 (purified monoclonal IgG2a, lyophilized)
T-2018 CD31 (PECAM-1; macrophage precursors), rat anti mouse, clone ER-MP12 Biotin (biotinylated monoclonal IgG2a, lyophilized)
AM20527SU-N CD31 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM20527SU-N CD31 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western Blot using anti-Human CD31 monoclonal antibody against CD31 recombinant protein
Formalin-Fixed, Paraffin-Embedded Human Placenta stained with CD31 / PECAM1 Antibody Cat.-No AM31062PU-N (Clone 1D2-1A5) at 5 µg/ml after heat-induced antigen retrieval.
Western Blot analysis of PECAM1 expression in human placenta using CD31 / PECAM1 Antibody Cat.-No AM31062PU-N (Clone 1D2-1A5).
A typical staining pattern with the B-B38 monoclonal antibody of platelets
A typical staining pattern with the B-B38 monoclonal antibody of platelets
A typical staining pattern with the B-B38 monoclonal antibody of platelets
A typical staining pattern with the B-B38 monoclonal antibody of platelets
Human Tonsil, Vessel: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using PECAM-1 Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells, using PECAM-1 Antibody. The lane on the right is treated with the synthesized peptide.
Formalin-Fixed, Paraffin-Embedded Human skin sections stained with CD31 antibody Cat.-No. AM10064SU-N using peroxidase conjugate and DAB chromogen. Note membranous staining of vascular endothelial cells.
Immunofluorescence analysis of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 antibody Cat.-No AM06169SU-N (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis of paraffin-embedded human placenta using CD31 antibody Cat.-No AM06169SU-N
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), lymphonodus tissue (B) and breast cancer (C), showing cytoplasmic localization of vascular endothelial cells using CD31 antibody Cat.-No AM06169SU-N with DAB staining.
Western blot analysis using CD31 antibody Cat.-No AM06169SU-N against truncated CD31 recombinant protein.
Staining of sheep peripheral blood platelets with Mouse anti Ovine CD31 Antibody Cat.-No SM464PS (Clone CO.3E1D4)
Human tonsil stained with Rabbit anti-CD31 / PECAM1 Antibody Cat.-No AM32446PU (Clone SP164).
Flow Cytometric analysis of Rabbit anti-CD31 / PECAM1 Antibody Cat.-No AM32446PU (Clone SP164) in Jurkat (green) compare to negative control of Rabbit IgG (blue).
Staining of FFPE Human colon cancer (10x and 40x) with CD31/PECAM1 Antibody Cat.-No AM32708PU-N (Clone BC16-6.4EF) at 2 µg/ml. Antibody positive in cytoplasm of interstitial endotelial cells modulating neoangiogenesis.
Human Umbilical Vein Endothelial Cells (HUVEC) stained with 5 µg/ml CD31/PECAM1 Antibody Cat.-No AM32708PU-N (Clone BC16-6.4EF) diluted in PBS with 1% BSA. FITC conjugated Rabbit anti Mouse Ig secondary.
CD31/PECAM1 Antibody Cat.-No AM32708PU-N (Clone BC16-6.4EF) staining of the vasculature in small round cell tumors. Indirect immunoperoxidase staining with Peroxidase conjugated anti-Mouse Ig secondary antibody. DAB substrate, hematoxylin counterstain.
Western blotting with anti CD31/PECAM1 Antibody Cat.-No AM32708PU-N (Clone BC16-6.4EF) used at 2 µg/ml (in Blotto) on HUVEC cell lysate loaded at 10, 5 and 2.5 µg/lane.
Secondary Antibody: Peroxidase conjugated Rabbit anti-Mouse Ig used at 1/10,000 dilution in Blotto.
Developed by enhanced chemiluminescence.
Secondary Antibody: Peroxidase conjugated Rabbit anti-Mouse Ig used at 1/10,000 dilution in Blotto.
Developed by enhanced chemiluminescence.
Western blot analysis of CD31 in THP-1 lysate probed with CD31 Antibody (Clone C31.7).
Formalin-Fixed, Paraffin-Embedded Human Angiosarcoma stained with CD31 Antibody Cat.-No AM32851PU (Clone C31.7).
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD31 Antibody Cat.-No AM32851PU (Clone C31.7).
Formalin-Fixed, Paraffin-Embedded Human liver (left) and prostate tissue (right) stained with CD31 Antibody Cat.-No AM32852PU (Clone 31.3). Note membrane staining.
Formalin-Fixed, Paraffin-Embedded Human lung squamous cell carcinoma (left) and bladder transitional cell carcinoma (right) stained with CD31 Antibody Cat.-No AM32852PU (Clone 31.3). Note membrane staining.
Formalin-Fixed, Paraffin-Embedded Human Angiosarcoma stained with CD31 Antibody Cat.-No AM32852PU (Clone 31.3).
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD31 Antibody Cat.-No AM32852PU (Clone 31.3). Note membrane staining.
Surface staining of lysed and wash human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE, BM4047R).
Surface staining of human peripheral blood with anti-human CD31 (MEM-05) biotin.
Paraffin Sections stained with CD31 Anitbody Cat.-No AM33193PU (Clone BS50)
Formalin-paraffin tonsil stained with CD31 Antibody Cat.-No AM50152PU (Clone SPM532)
Formalin-fixed, paraffin-embedded human tonsil stained with CD31 Antibody CAt.-No AM50153PU (Clone SPM122).
Formalin-Paraffin Human Angiosarcoma stained with CD31 Antibody Cat.-No AM50225PU (Clone C31.7+JC/70A)
Formalin-Paraffin tonsil stained with CD31 Antibody Cat.-No AM50225PU (Clone C31.7+JC/70A)
Formalin-Fixed, Paraffin-Embedded Human Angiosarcoma stained with CD31 Antibody Cat.-No AM50226PU (Clone JC/70A).
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD31 Antibody Cat.-No AM50226PU (Clone JC/70A).
Western blot analysis of extracts from Jurkat tissue using PECAM-1 (Phospho-Tyr713) antibody AP55942PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of CD31 (pY713) expression in HeLa (A); Jurkat (B); K562 (C); NIH3T3 (D); PC12 (E) whole cell lysates.
Western blot analysis of CD31 expression in Jurkat (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Representative Standard Curve
Representative Standard Curve
Flow cytometric analysis of human platelet using Anti-CD31 Antibody-FITC labled.
Flow cytometric analysis of human platelet using Anti-CD31 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood platelets using Anti-CD31 Antibody; followed by anti-mouse IgG PE.
Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-PECAM1 (TA000121) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1(RC208654) (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504759)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504759)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504759)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504759)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504759)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504762)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504762)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504762)
Anti-PECAM1 mouse monoclonal antibody (TA504762) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504762), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504763)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504763), and then analyzed by flow cytometry.
Anti-PECAM1 mouse monoclonal antibody (TA504773) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PECAM1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504773), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504774)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504774)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504774)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504774)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504798)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504803)
Anti-PECAM1 mouse monoclonal antibody (TA504803) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504803), and then analyzed by flow cytometry.
Anti-PECAM1 mouse monoclonal antibody (TA504804) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-PECAM1 monoclonal antibody at 1:200.
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504804), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504811)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-PECAM1 monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504812)
Anti-PECAM1 mouse monoclonal antibody (TA504812) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PECAM1 monoclonal antibody at 1:200.
Flow cytometric analysis of live Jurkat cells, using anti-PECAM1 antibody (TA504812 at 1:100, red), compare to IgG2b isotype control (Black).
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504812), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504841)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504842)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504842)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504842)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504842)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504842)
Anti-PECAM1 mouse monoclonal antibody (TA504842) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC208654 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PECAM1 antibody(TA504842), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504861)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504861)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504861)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western blot analysis of extracts (10ug) from 1 cell line by using anti-PECAM1 monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Flow cytometric Analysis of HUVEC cells, using anti-PECAM1 antibody(TA504862),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504880)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504880)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504880)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504880)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504891)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504891)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504891)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504891)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PECAM1 monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504922)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-PECAM1 monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504964)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504964)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504964)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505003)
Anti-PECAM1 mouse monoclonal antibody (TA505003) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1(RC208654).
Western blot analysis of extracts (10ug) from 1 cell line by using anti-PECAM1 monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
ELISA: CD31 Antibody
PECAM1 Luminex Elisa with 1B10 Capture (TA600244) and 2C6 Detection (TA700244) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 1B10 Capture (TA600244) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4C1 Capture (TA600245) and 2C6 Detection (TA700244) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4C1 Capture (TA600245) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 2C6 Capture (TA600246) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 3B5 Capture (TA600247) and 1D2 Detection (TA700246) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 3B5 Capture (TA600247) and 2C6 Detection (TA700244) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 3B5 Capture (TA600247) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4D1 Capture (TA600248) and 1D2 Detection (TA700246) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4D1 Capture (TA600248) and 3B5 Detection (TA700247) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 1D2 Capture (TA600249) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 2B5 Capture (TA600250) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 2B5 Capture (TA600250) and 3B5 Detection (TA700247) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 1B10 Capture (TA600244) and 2C6 Detection (TA700244) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 3B5 Capture (TA600247) and 2C6 Detection (TA700244) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4C1 Capture (TA600245) and 2C6 Detection (TA700244) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 1D2 Capture (TA600249) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 1B10 Capture (TA600244) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 2B5 Capture (TA600250) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 2C6 Capture (TA600246) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4C1 Capture (TA600245) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 3B5 Capture (TA600247) and 2E4 Detection (TA700245) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 3B5 Capture (TA600247) and 1D2 Detection (TA700246) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4D1 Capture (TA600248) and 1D2 Detection (TA700246) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 2B5 Capture (TA600250) and 3B5 Detection (TA700247) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
PECAM1 Luminex Elisa with 4D1 Capture (TA600248) and 3B5 Detection (TA700247) Antibodies. Substrate used: Recombinant Human PECAM1 (TP308654)
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (Clone UMAB29). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 (Clone UMAB29) very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (Clone UMAB30). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 (Clone UMAB30) very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (Clone UMAB32). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 (Clone UMAB32) very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (UM500106). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Western Blot: CD31 Antibody - WB detection of CD31 in HUVEC cell lysates.
Immunohistochemistry: CD31 Antibody - IHC staining of CD31 in mouse brain.
Western blot analysis of extracts from Jurkat cells, using PECAM-1 (Ab-713) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COS7 cells (Lane 2) and JK cells (Lane 3), using PECAM-1 (Ab-713) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PECAM-1 (Ab-713) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using PECAM-1 (Ab-713) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from mouse brain cells, using GAD1/2 Antibody (#TA313340).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using GAD1/2 Antibody (#TA313340).The picture on the right is treated with the synthesized peptide.
Staining of mouse splenocytes with Anti-Mouse CD31 (PECAM-1) FITC (left) and PE (right). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
Surface staining of normal human peripheral blood cells with Anti-Human CD31 (PECAM-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.
Western blot analysis of PECAM-1 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Immunohistochemistry staining of Paraffin Tonsil tissue by CD31 antibody (dilution: 1:25 - 1:100; visualization of staining: Cytoplasmic, membranous)
Flow Cytometry: CD31/PECAM1 Antibody (MEC 7.46) [TA336264] - CD31/PECAM1 (MEC 7.46) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
Immunocytochemistry/Immunofluorescence: CD31/PECAM1 Antibody (MEC13.3) [TA336675] - CD31/PECAM1 (MEC13.3) antibody was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Flow Cytometry: CD31/PECAM1 Antibody (MEC13.3) [TA336675] - C57BL/6 mouse splenocytes stained with purified MEC13.3, followed by anti-rat IgG FITC
Western Blot: CD31/PECAM1 Antibody (JC/70A) [TA336692] - Western blot analysis of CD31/PECAM1 expression in 1) Jurkat whole cell lysate, 2) human platelet lysate and 3) U937 whole cell lysate.
Immunohistochemistry-Frozen: CD31/PECAM1 Antibody (JC/70A) [TA336692] - Rabbit Heart, CD31 Stained Red. Image from verified customer review.
Immunohistochemistry-Paraffin: CD31 Antibody (JC/70A) [TA336692] - Formalin fixed paraffin embedded human tonsil stained with CD31 antibody.
Immunohistochemistry-Paraffin: CD31/PECAM1 Antibody (JC/70A) [TA336692] - IHC analysis of CD31/PECAM1 in human spleen using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: CD31/PECAM1 Antibody (JC/70A) [TA336692] - Formalin fixed paraffin embedded human tonsil stained with CD31, using ABC method.
Immunocytochemistry/Immunofluorescence: CD31/PECAM1 Antibody (JC/70A) [TA336692] - The CD31/PECAM antibody was tested in Huvec cells at a 1:10 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAP
Western blot analysis on Jurkat cell lysate using PECAM-1 Antibody
C57BL/6 splenocytes stained with 390 PE
HUVEC cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 10 ug/ml of purified anti-CD31 (clone WM59) overnight, followed by 2.5 ug/ml DyLight™ 594 anti-mouse IgG for 2 hours at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
Human peripheral blood lymphocytes, monocytes and granulocytes stained with purified WM59, then detected with anti-mouse IgG FITC
Western blot analysis of PECAM-1 phosphorylation expression in Jurkat whole cell lysates, the lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells, using PECAM-1 (Phospho-Tyr713) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using PECAM-1 (Phospho-Tyr713) antibodyThe picture on the right is treated with the synthesized peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500121 | CD31 / PECAM1 antibody |
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| anti-PECAM1 (CD31) mouse monoclonal antibody, clone OTI8A9 (formerly 8A9) | |||||||
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Mouse | IgG2a | OTI8A9 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
Top Selling product!
|
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| TA504773 | CD31 / PECAM1 antibody |
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| PECAM1 (CD31) mouse monoclonal antibody, clone OTI1H6 (formerly 1H6) | |||||||
 |
Mouse | IgG1 | OTI1H6 | Purified | Can, Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Top Selling product! Highly validated antibody!
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| BM4086 | CD31 / PECAM1 antibody |
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 |
Rat | IgG2a | ER-MP12 | Purified | Ms | C, F, P |
0.1 mg /
€480.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Top Selling product! Published recently! |
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| BM4047 | CD31 / PECAM1 antibody |
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 |
Mouse | IgG1 | MEM-05 | Hu | C, F, P |
0.2 mg /
€310.00
|
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| Acris Antibodies GmbH | |||||||
| +2 additional images |
Published recently! |
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| BM4047APC | CD31 / PECAM1 antibody |
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 |
Mouse | IgG1 | MEM-05 | APC | Hu | F |
100 Tests /
€290.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Published recently! |
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| BM4047B | CD31 / PECAM1 antibody |
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 |
Mouse | IgG1 | MEM-05 | Biotin | Hu | F |
0.1 mg /
€260.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Published recently! |
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| BM4047F | CD31 / PECAM1 antibody |
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Mouse | IgG1 | MEM-05 | FITC | Hu | F |
100 Tests /
€260.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Published recently! |
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| BM4047R | CD31 / PECAM1 antibody |
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Mouse | IgG1 | MEM-05 | PE | Hu | F |
100 Tests /
€290.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Published recently! |
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| BM4047S | CD31 / PECAM1 antibody |
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 |
Mouse | IgG1 | MEM-05 | Purified | Hu | F, WB |
0.1 mg /
€210.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Published recently! |
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| BM4086B | CD31 / PECAM1 antibody |
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 |
Rat | IgG2a | ER-MP12 | Biotin | Ms | C, F, P |
0.3 mg /
€500.00
|
| Acris Antibodies GmbH | |||||||
Published recently! |
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| UM500029 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB29 | |||||||
 |
Mouse | IgG2a | UMAB29 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| UM500030 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB30 | |||||||
 |
Mouse | IgG1 | UMAB30 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| UM500031 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB31 | |||||||
 |
Mouse | IgG1 | UMAB31 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| UM500032 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB32 | |||||||
 |
Mouse | IgG1 | UMAB32 | Purified | Hu | P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| UM500106 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB164 | |||||||
 |
Mouse | IgG1 | UMAB164 | Purified | Hu | P |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| UM570029 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB29 | |||||||
|
Mouse | IgG2a | UMAB29 | Purified | Hu | P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| UM570030 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB30 | |||||||
|
Mouse | IgG1 | UMAB30 | Purified | Hu | P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| UM570031 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB31 | |||||||
|
Mouse | IgG1 | UMAB31 | Purified | Hu | P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| UM570032 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB32 | |||||||
|
Mouse | IgG1 | UMAB32 | Purified | Hu | P, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| UM570106 | CD31 / PECAM1 antibody |
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| PECAM1 mouse monoclonal antibody,clone UMAB164 | |||||||
|
Mouse | IgG1 | UMAB164 | Purified | Hu | P |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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