CD317 / BST2 antibody

Principal name

CD317 / BST2 antibody

Alternative names for CD317 / BST2 antibody

Bone marrow stromal antigen 2, BST-2, Tetherin, HM1.24 antigen

SwissProt ID

Q10589 (Human), Q811A2 (Rat), Q8R2Q8

Gene ID

684 (BST2), 69550 (Bst2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate)

Available hosts

Rabbit, Rat, Mouse, Goat, Hamster

Available applications

Dot blot (Dot), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), EPair for Elisa (EPair), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of CD317 / BST2 antibody

Bone marrow stromal Antigen-2 (BST-2) is a 30- to 36-kDa type II transmembrane protein, consisting of 180 amino acids. It has been shown to form a complex with Rho-GTPase activating protein, RICH2 (1). This complex is essential for the organization of subapical actin cytoskeleton. BST-2 protein is expressed on certain bonemarrow stromal cell lines and on various normal tissues. The expression pattern of BST2 is relatively different form another bone marrow stromal antigen 1 (BST1). The BST-2 gene is located on chromosome 19p13.2. BST-2 surface expression on fibroblast cell lines facilitated the stromal cell-dependent growth of a murine bone marrow-derived pre-B-cell line, DW34. Some studies suggest that BST-2 may be involved in pre-B-cell growth development by regulating their surface molecules and cytokines (2). BST2 is also expressed in synovial cell lines from where the BST2 gene was cloned. BST2 is predominantly expressed in liver, lungs, heart, placenta and lower levels in pancreas, kidney, skeletal muscle and brain. Naturally all human cells possess an antiviral activity that inhibits the release of retrovirus particles, and other enveloped virus particles. This antiviral activity is antagonized by the HIV-1 accessory protein, Vpu. The antiviral activity can be constitutively induced and expressed by interferon-alfa and is consist of protein based “tetherins” that cause retention of fully formed virions on infected cell surface. BST2 (CD317) was identified as tetherin whose expression correlated with and induced, a requirement for Vpu during HIV-1 and murine leukaemia virus particle release. Vpu and BST2 are co localized in the same cell compartments. Further depletion of BST2 in cells that require Vpu induction for HIV-1 virion release, abolish VPU role in particle release (3). Manipulation of Vpu function and consequent mobilization of tetherin’s antiviral activity could be a potential therapeutic strategy in HIV/AIDS infection. Interferon alfa also enhances BST2 expression and antitumor activity of anti-BST2 antibody in renal cell carcinoma xenograft models (4).

General readings

1. Rollason R, et al. A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. J Cell Biol. 9 March 2009; 184(5):721-736.
2. Ishikawa J, et al. Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth. Genomics. 1995 Apr 10; 26(3):527-34.
3. Nature. 2008 Jan 24; 451(7177):425-30. Epub 2008 Jan 16.
4. Kawai S, et al. Interferon-alpha enhances CD317 expression and the antitumor activity of anti-CD317 monoclonal antibody in renal cell carcinoma xenograft models. Cancer Sci. 2008 Dec; 99(12):2461-6.
5. Perez-Caballero D, Zang T, Ebrahimi A, McNatt MW, Gregory DA, Johnson MC, Bieniasz PD, Tetherin inhibits HIV-1 release by directly tethering virions to cells. Cell 2009 Oct 139 (3): 499-511.
PMID: 19879838

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD317 / BST2 antibody

Western Blot: BST2 Antibody (2E2) [TA337180] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on © partial recombinant BST2 protein with monoclonal BST2 antibody at a concentration of 1 ug/ml. In the tested human Mouse IgG1 2E2 Purified Hu C, F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD317 / BST2 antibody

Immunoperoxidase of purified MaxPab antibody to BST2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Alexa Fluor 488 Ms F, ICC/IF
50 µg / €390.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid dendritic cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Alexa Fluor 488 Ms F, ICC/IF
0.1 mg / €520.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Alexa Fluor 546 Ms ICC/IF
50 µg / €390.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid dendritic cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Alexa Fluor 546 Ms ICC/IF
0.1 mg / €520.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Alexa Fluor 647 Ms F
50 µg / €390.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid dendritic cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Alexa Fluor 647 Ms F
0.1 mg / €520.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Biotin Ms C, F
50 µg / €360.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Biotin Ms C, F
0.1 mg / €480.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Azide Free Ms FN
1 mg / €790.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid Dendritic Cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Azide Free Ms FN
5 mg / €2,560.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid dendritic cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Purified Ms C, F, FN
50 µg / €330.00
  Dendritics SAS

CD317 / BST2 (Plasmacytoid dendritic cells / pDCs) antibody

IHC staining of murine spleen cryosections Rat IgG1 120G8.04 Purified Ms C, F, FN
0.1 mg / €440.00
  Dendritics SAS

CD317 / BST2 (N-term) antibody

Western blot analysis of Bst2 in Daudi cell lysate with Bst2 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD317 / BST2 antibody

(0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD317 / BST2 antibody

Western blot analysis of extracts from Jurkat cells, using BST2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD317 / BST2 antibody

Western Blot: BST2 Antibody [TA337141] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human BST2 protein probed with BST2 antibody at 4 ug/ml. Go Rabbit Purified Hu, Ms, Prim, Rt F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD317 / BST2 antibody

Western Blot: BST2 Antibody (4F6) [TA337181] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on © partial recombinant BST2 protein with monoclonal BST2 antibody (clone 4F6) at a concentration of 1 ug/ml. In the Mouse IgG1 4F6 Purified Hu C, F, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD317 / BST2 antibody

ELISA: BST2 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

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