CD329 / SIGLEC8 antibody

Principal name

CD329 / SIGLEC8 antibody

Alternative names for CD329 / SIGLEC8 antibody

Siglec-8, CDw329, SAF2, Sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2

SwissProt ID

Q9NYZ4 (Human)

Gene ID

27181 (SIGLEC8)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of CD329 / SIGLEC8 antibody

Kit Component:
- KN208582G1, SIGLEC8 gRNA vector 1 in pCas-Guide vector
- KN208582G2, SIGLEC8 gRNA vector 2 in pCas-Guide vector
- KN208582D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS.
Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
J Allergy Clin Immunol. 2008 Feb;121(2):499-505.e1. Epub 2007 Nov 26.
PMID: 18036650

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD329 / SIGLEC8 antibody

  Goat Anti-SIGLEC8 Antibody  
TA305896 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD329 / SIGLEC8 antibody

  Rabbit Polyclonal Anti-SIGLEC8 Antibody  
Gel: 6%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (SIGLEC8 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD329 / SIGLEC8 antibody

  Rabbit Polyclonal Anti-SIGLEC8 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (SIGLEC8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD329 / SIGLEC8 antibody

  Mouse monoclonal Anti-SIGLEC8 Clone 7C9  
CD329 / SIGLEC8 Mouse IgG1 7C9 Purified Hu E, F, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD329 / SIGLEC8 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SIGLEC8 - human gene knockout kit via CRISPR

SIGLEC8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIGLEC8 - human gene knockout kit via CRISPR

SIGLEC8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIGLEC8 - human gene knockout kit via CRISPR

SIGLEC8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIGLEC8 - human gene knockout kit via CRISPR

SIGLEC8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD329 / SIGLEC8

  Recombinant protein of human sialic acid binding Ig-like lectin 8 (SIGLEC8)  
CD329 / SIGLEC8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SIGLEC8 overexpression lysate

SIGLEC8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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