CD329 / SIGLEC8 antibody

Principal name

CD329 / SIGLEC8 antibody

Alternative names for CD329 / SIGLEC8 antibody

Siglec-8, CDw329, SAF2, Sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2

SwissProt ID

Q9NYZ4 (Human)

Gene ID

27181 (SIGLEC8)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of CD329 / SIGLEC8 antibody

SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.

General readings

Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS.
Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement.
J Allergy Clin Immunol. 2008 Feb;121(2):499-505.e1. Epub 2007 Nov 26.
PMID: 18036650

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD329 / SIGLEC8 antibody

TA305896 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD329 / SIGLEC8 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD329 / SIGLEC8 antibody

CD329 / SIGLEC8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8 antibody

CD329 / SIGLEC8 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8 antibody

Detection limit for recombinant GST tagged SIGLEC8 is approximately 3ng/ml as a capture antibody. Mouse IgG2b 3H1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SIGLEC8 polyclonal antibody ( Cat # PAB3640 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8 antibody

SIGLEC8 polyclonal antibody ( Cat # PAB11442 ) ( 0.3 µg/ml ) staining of MOLT 4 lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8 antibody

NBP1-06077(0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

CD329 / SIGLEC8 antibody

Western Blot: SIGLEC8 Antibody [NBP1-31141] - Western blot analysis of SIGLEC8 Antibody on: A: A431 7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

CD329 / SIGLEC8 antibody

Immunohistochemistry-Paraffin: SIGLEC8 Antibody [NBP1-82666] Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD329 / SIGLEC8

CD329 / SIGLEC8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD329 / SIGLEC8

CD329 / SIGLEC8 Human
10 µg / €540.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8

CD329 / SIGLEC8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

CD329 / SIGLEC8

CD329 / SIGLEC8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SIGLEC8 overexpression lysate

SIGLEC8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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