CD33 / SIGLEC3 antibody

Principal name

CD33 / SIGLEC3 antibody

Alternative names for CD33 / SIGLEC3 antibody

Siglec-3, gp67, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3

SwissProt ID

P20138 (Human), Q63994 (Mouse)

Gene ID

945 (CD33), 12489 (Cd33)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Luminex (LMNX)

Background of CD33 / SIGLEC3 antibody

CD33/SIGLEC3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,6-linked sialic acid; the sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, SIGLEC3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. This protein Induces apoptosis in acute myeloid leukemia (in vitro). It has been shown to interact with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SIGLEC3 expresses in monocytic/myeloid lineage cells, and contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. The gene for SIGLEC3 belongs to the immunoglobulin superfamily.

General readings

Freeman SD, Kelm S, Barber EK, et al. Characterization of CD33 as a new member of the sialoadhesion family of cellular interaction molecules. Blood 1995; 85:2005-12.
Crocker PR and Varki A. Siglecs, sialic acids, and innate immunity. Trends Immunol. 2001; 22:337-42.
Taylor VC, Buckley CD, Douglass M, et al. The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. J. Biol. Chem. 1999; 274:11505-12
Naj AC, Jun G, Beecham GW, et al. Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA are associated with late-onset Alzheimer’s disease. Nat. Genet. 2011; 43:436-41

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD33 / SIGLEC3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD33 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506294) Mouse IgG1 OTI2C1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

CD33 ELISA with 1E4 Capture (TA600373) and 2F8 Detection (TA700373) Antibodies. Substrate used: Recombinant Human CD33 (TP307023) Mouse IgG1 OTI1E4 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

CD33 ELISA with 1E4 Capture (TA600373) and 1B10 Detection (TA700372) Antibodies. Substrate used: Recombinant Human CD33 (TP307023) Mouse IgG1 OTI1B10 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 (Center) antibody

Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

CD33 / SIGLEC3 Mouse HIM3-4 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD33 Purified (filled histogram) followed by Anti-Mouse IgG FITC and granulocyte (right) gates were used for analysis. Mouse WM-53 (WM53) Purified Hu F, ICC/IF, IP
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

Western blot analysis of extracts of various cell lines, using CD33 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

Immunohistochemistry staining of Paraffin Acute myeloid leukemia with monocytic differentiation, placenta tissue by CD33 antibody (dilution: 1:20 - 1:80; visualization of staining: Membranous) Mouse IgG2b PWS44 Purified Hu P
1 ml / €820.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

Western Blot: CD33 Antibody (44M12D3) [TA336936] - analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this Mouse IgG2a 44M12D3 Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD33 / SIGLEC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD33 (RC207023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD33. Mouse IgG1 OTI1H8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI1E10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506320) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI1B10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506328) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI2F8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD33 (RC207023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD33. Mouse IgG1 OTI2E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD33 (RC207023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD33. Mouse IgG2b OTI2G8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506335) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI1G10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506347) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI2A1 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD33 (RC207023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD33. Mouse IgG1 OTI1E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506349) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI1C4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD33 / SIGLEC3 antibody

Anti-CD33 mouse monoclonal antibody (TA506350) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD33(RC207023). Mouse IgG1 OTI2B2 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

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