CD34 antibody

Principal name

CD34 antibody

Alternative names for CD34 antibody

Hematopoietic progenitor cell marker, Hematopoietic progenitor cell antigen CD34

SwissProt ID

P28906 (Human), Q28270 (Canfa), Q64314 (Mouse)

Gene ID

947 (CD34), 12490 (Cd34), 415130

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Can (Canine), Prim (Primate), Rt (Rat), Rhesus Mky (Rhesus Monkey)

Available hosts

Mouse, Rat, Chicken, Rabbit

Available applications

Frozen Sections (C), Functional assay (FN), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT), ELISA (detection) (E(detection))

Background of CD34 antibody

CD34 is a type I transmembrane glycoprotein (Mr = 104-120 kDa) expressed on stem cells, early hematopoietic progenitor cells, bone marrow stroma cells, endothelial cells, embryonic fibroblasts, and neurons.  CD34 analysis can be used for the differential identification of acute leukemias as well as routine analysis of cultured stem cell lines. 
CD34 a type 1 transmembrane protein, belonging to the sialomucin family, has important roles in adhesion which remain to be fully elucidated.  CD34 has an intracellular cytoplasmic domain containing consensus sites for serine, theronine, tryrosine and active protein kinase C (PKC) phosphorylation thereby implicating a role for the protein in signal transduction processes.  CD34 expression, as identified by antibody, is a hallmark for identifying pluripotential hematopoietic stem or progenitor cells.   CD34 antibody positive populations expand and differentiate into the various lymphohematopoietic lineages.  During differentiation, lineages lose CD34 expression and become CD34 antibody negative.
CD34 is also expressed on vascular endothelium, bone marrow stroma, embryonic fibroblasts, and neurons and antibody to CD34 is also useful in analyzing these populations.  Positive staining has also been described in a number of malignancies including various sarcomas and fibromas, preB-ALL, peripheral nerve sheath tumors, and papillary thyroid carcinoma.  If certain kinds of tumors are characteristically positive for CD34 antibody staining such as Ewing’s sarcoma, a CD34 antibody negative result may potentially rule out that particular tumor.

General readings

1. Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10(6):863-71.
2. Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan
1;101(1):112-8.
3. Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14(5):470-7.
4. Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15(3):305-13.
5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
6. Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P: Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007;54(4):311-20.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD34 antibody

Figure from citation: Immunofluorescence of CD34 protein level by using anti-CD34 antibody in human pulmonary arterial endothelial cells. Dilution: 1:100 View Citation Mouse IgG2a OTI51A1 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD34 antibody

Staining of CLL-1390 cells with Mouse anti Canine CD34:RPE (Cat.-No SM2257R). Mouse IgG1 1H6 PE Can F
100 Tests / €590.00
  Acris Antibodies GmbH

CD34 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808864)(1:150) Mouse IgG2a OTI12F2 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD34 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808885)(1:150) Mouse IgG2a OTI11E1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD34 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808886)(1:150) Mouse IgG2a OTI11A5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD34 (N-term) antibody

Focal CD34 positivity in the GIST (gastrointestinal stromal tumor) of small intestine (A) and in the endothelium of the muscular artery (B), stained with DB 151 monospecific antibody.
Kindly performed and provided by Marián Švajdler, MD and Dr. Lucia Fröhlichová, from Louis Pasteur University Hospital Department of Pathology, Košice, Slovak Republic. Rabbit IgG Q19-E Aff - Purified Hu P
0.1 ml / €270.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD34 (N-term) antibody

Focal CD34 positivity in the GIST (gastrointestinal stromal tumor) of small intestine (A) and in the endothelium of the muscular artery (B), stained with DB 151 monospecific antibody.Kindly performed and provided by Marián Švajdler, MD and Dr. Lucia Fröhlichová, from Louis Pasteur University Hospital Department of Pathology, Košice, Slovak Republic. Rabbit IgG Q19-E Aff - Purified Hu P
0.2 ml / €360.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD34 (N-term) antibody

Focal CD34 positivity in the GIST (gastrointestinal stromal tumor) of small intestine (A) and in the endothelium of the muscular artery (B), stained with DB 151 monospecific antibody.Kindly performed and provided by Marián Švajdler, MD and Dr. Lucia Fröhlichová, from Louis Pasteur University Hospital Department of Pathology, Košice, Slovak Republic. Rabbit IgG Q19-E Aff - Purified Hu P
0.5 ml / €460.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD34 (N-term) antibody

Focal CD34 positivity in the GIST (gastrointestinal stromal tumor) of small intestine (A) and in the endothelium of the muscular artery (B), stained with DB 151 monospecific antibody.Kindly performed and provided by Marián Švajdler, MD and Dr. Lucia Fröhlichová, from Louis Pasteur University Hospital Department of Pathology, Košice, Slovak Republic. Rabbit IgG Q19-E Aff - Purified Hu P
1 ml / €700.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD34 Class II antibody

Human lung stained with anti-CD34 antibody Mouse IgG1 QBEND/10 Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD34 Class II antibody

Human lung stained with anti-CD34 antibody Mouse IgG1 QBEND/10 Purified Hu P
1 ml / €510.00
  Acris Antibodies GmbH

CD34 Class II antibody

Human lung stained with anti-CD34 antibody Mouse IgG1 QBEND/10 Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD34 antibody

Formalin-Paraffin Human tonsil (20X) stained with CD34 Antibody Cat.-No AM50193PU (Clone SPM123). Mouse IgG1 SPM123 Purified Hu, Mky C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD34 antibody

Formalin-Paraffin Human tonsil (20X) stained with CD34 Antibody Cat.-No AM50193PU (Clone SPM123). Mouse IgG1 SPM123 Purified Hu, Mky C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD34 antibody

Formalin-Paraffin Human tonsil (20X) stained with CD34 Antibody Cat.-No AM50193PU (Clone SPM123). Mouse IgG1 SPM123 Purified Hu, Mky C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD34 (Endothelial Cell Marker) antibody

Human angiosarcoma stained with anti-CD34 antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD34 (Endothelial Cell Marker) antibody

Human angiosarcoma stained with anti-CD34 antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD34 (Endothelial Cell Marker) antibody

Human angiosarcoma stained with anti-CD34 antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD34 antibody

Staining of TF-1 cell line with 0.25 ug of Mouse IgG1 K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD34 Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Total viable cells were used for analysis. Mouse 4H11 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD34 antibody

Predicted band size: 110 kDa . Positive control: HUVEC cells and Hematopoietic Stem Cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: HUVEC cells and Hematopoietic Stem Cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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