CD349 / FZD9 / Frizzled-9 antibody
Principal name
CD349 / FZD9 / Frizzled-9 antibody
Alternative names for CD349 / FZD9 / Frizzled-9 antibody
Fz-9, hFz9
Ncbi ID
NP_003499.1, NP_003499, 9606, ABM84300.1, NM_003508, NP_034376.1, NM_010246
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of CD349 / FZD9 / Frizzled-9 antibody
The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. The frizzled-9 gene is located within the Williams Syndrome common deleted region at chromosomal band 7q11.23. Heterozygous deletion of the frizzled-9 gene may contribute to the Williams Syndrome phenotype. In mouse, frizzled-9 overexpression can induce the hyperphosphorylation and relocalization of Dvl-1 from the cytoplasm to the cell membrane and cytosolic β-catenin accumulation. In rat, frizzled-9 is important in Wnt/β-catenin signaling in 293T cells. Frizzled-9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.
General readings
Winn,R.A., (2005) J. Biol. Chem. 280 (20), 19625-19634
Immunohistochemical staining of Brain (Purkinje neurons) using anti- FZD9 antibody SP4154P
Human Intestine; Rabbit Anti-FZD9 Antibody. Catalog Number: ARP41253. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; FZD9 antibody - N-terminal region (ARP41253_T100) in Human Intestine cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-FZD9 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; FZD9 antibody - N-terminal region (ARP41253_T100) in Human Jurkat cells using Western Blot
Immunohistochemistry: AP06970PU-N FZD9 antibody staining of Formalin-Fixed Paraffin-Embedded Human Testis at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Testis: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot (WB) analysis of Frizzled-9 antibody (Cat.-No.: AP01300PU-N) in extracts from HUVEC cells.
Human Brain; WB Suggested Anti-FZD9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; FZD9 antibody - N-terminal region (ARP41252_P050) in Human Brain cells using Western Blot
AP31110PU-N Frizzled-9 antibody staining of Formalin-Fixed, Paraffin Embedded Human Testis at 5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of Frizzled 9 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Western blot analysis of Frizzled 9 expression in Y79 (A) whole cell lysates.
Immunofluorescent analysis of Frizzled 9 staining in Y79 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of extracts from HUVEC cells COLO cells and COS cells, using FZD9 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using FZD9 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells, using FZD9 antibody.The lane on the right is treated with the synthesized peptide.
Anti-FZD9 / Frizzled 9 antibody IHC of human brains, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD9 / Frizzled 9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD9 / Frizzled 9 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD9 / Frizzled 9 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD9 / Frizzled 9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD9 / Frizzled 9 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
WB Suggested Anti-FZD9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain
WB Suggested Anti-FZD9 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate
Rabbit Anti-FZD9 Antibody; Paraffin Embedded Tissue: Human Intestine; Cellular Data: Epithelial cells of intestinal villas; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314730 | CD349 / FZD9 / Frizzled-9 antibody |
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| Rabbit polyclonal anti-FZD9 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA314731 | CD349 / FZD9 / Frizzled-9 antibody |
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| Rabbit polyclonal anti-FZD9 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA340718 | CD349 / FZD9 / Frizzled-9 (N-term) antibody |
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| Rabbit Polyclonal Anti-FZD9 Antibody (N-Terminus) | |||||||
 |
Rabbit | Aff - Purified | Bov, Can, Hst, Hu, Ms, Rb, Rt | P |
50 µg /
€400.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA340951 | CD349 / FZD9 / Frizzled-9 antibody |
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| Rabbit Polyclonal Anti-FZD9 Antibody (Extracellular Domain) | |||||||
 |
Rabbit | Purified | Hu |
50 µg /
€400.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA340954 | CD349 / FZD9 / Frizzled-9 antibody |
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| Rabbit Polyclonal Anti-FZD9 Antibody (N-Terminus) | |||||||
 |
Rabbit | Purified | Bov, Hu, Ms, Rt |
50 µg /
€400.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA344066 | CD349 / FZD9 / Frizzled-9 antibody |
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| Rabbit Polyclonal Anti-FZD9 Antibody | |||||||
 |
Rabbit | IgG | Purified | Can, GP, Hu, Ms, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344067 | CD349 / FZD9 / Frizzled-9 antibody |
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| Rabbit Polyclonal Anti-FZD9 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Rb, Rt, Ze | P, WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP01300PU-N | CD349 / FZD9 / Frizzled-9 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| AP06970PU-N | CD349 / FZD9 / Frizzled-9 (N-term, Extracel. Dom.) antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Hu, Ms, Platypus, Rb, Rt | E, P |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| AP06971PU-N | CD349 / FZD9 / Frizzled-9 (3rd Extracell. Dom.) antibody |
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 |
Rabbit | Aff - Purified | Hu | E, P |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| AP31110PU-N | CD349 / FZD9 / Frizzled-9 antibody |
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 |
Goat | Aff - Purified | Hu, Ms, Rt | E, P |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| SP4154P | CD349 / FZD9 / Frizzled-9 (N-term, Extracel. Dom.) antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Hu, Platypus | ICC/IF, P |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| G114-1 | CD349 / FZD9 / Frizzled-9 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
50 µg /
€230.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
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| G114-2 | CD349 / FZD9 / Frizzled-9 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€330.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205325 | FZD9 - human gene knockout kit via CRISPR |
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|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205325BN | FZD9 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205325LP | FZD9 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205325RB | FZD9 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN306211 | Fzd9 - mouse gene knockout kit via CRISPR |
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|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN306211BN | Fzd9 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
