CD36 antibody
Principal name
CD36 antibody
Alternative names for CD36 antibody
Glycoprotein IIIb, PAS IV, PAS-4, Thrombospondin receptor, GP3B, GP4, Platelet glycoprotein 4, Platelet glycoprotein IV, Platelet collagen receptor, Fatty acid translocase
Ncbi ID
9606, NP_001001547, NP_000063, NP_001153027, NP_000063.2, NP_001153027.1, 10090, NP_031669, NP_001001548, NP_001120915, NP_001120916, NM_000072, NM_001159555, NM_001159557, NM_001159558, NM_001159556, NM_001001548, NM_001127443, NM_001127444
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Functional assay (FN), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Luminex (LMNX)
Background of CD36 antibody
CD36 is a heavily N-glycosylated transmembrane protein of ~88 kDa with two short intracellular domains and a large extracellular domain. The protein is sensitive for neuroaminidase, resulting in a shift from 88 to 85 kDa. CD36 is expressed on platelets, mature monocytes and macrophages, microvascular endothelial cells, mammary endothelial cells, during stages of erythroid cell development and on some macrophage derived dendritic cells. The antibody recognizes adult and fetal monocytes, platelets and reticulocytes, but doesn’t stain lymphocytes and granulocytes. Reactivity has also been found in small intestine, kidney, liver and thyroid. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adaptor molecules. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum-infected red blood cells. CD36 is associated with Src-family kinases and with the integrins α3β1 and α6β1. Recently, CD36 has been identified as a protein that is required for toll like receptor (TLR2) recognition of di-acylated bacterial lipopeptides and lipoteichoic acid4. Furthermore, CD36 has been shown to function as phagocytic receptor for apoptotic cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues.
General readings
Febbraio M., et al. (2001) J. Clin. Invest. 108:785-791.Armstrong LC., et al. (2003) Matrix Biol. 22:63-71.
Figure 1. SM1769A staining of human peripheral blood monocytes.
Flow Cytometry: Staining of Human peripheral blood platelets with Mouse anti Human CD36 antibody (SM1769P)
Surface staining of Human platelets with anti-CD36 antibody (TR9) FITC.
Surface staining of human platelets with anti-CD36 (TR9) FITC.
Surface staining of human platelets with anti-CD36 (TR9) FITC.
Human Liver; . Rabbit Anti-CD36 Antibody. . Catalog Number:. ARP48129 . Paraffin Embedded Tissue: Human hepatocyte . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human HepG2; Immunohistochemistry with HepG2 cell lysate tissue at an antibody concentration of 1 ug/ml using anti-CD36 antibody (ARP48129_P050); CD36 antibody - N-terminal region (ARP48129_P050) in Human HepG2 cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-CD36 Antibody Titration: 1 ug/ml. Positive Control: HepG2 cell lysate; CD36 antibody - N-terminal region (ARP48129_P050) in Human HepG2 cells using Western Blot
Flow Cytometry analysis of P3U1 cells stained with CD36 monoclonal antibody, clone NF189 ( Cat # MAB1709 ) .
Staining of CD36 on human adipocytes using CD36 polyclonal antibody ( Cat # PAB12463 ) .
Detection of CD36 in human adipocyte extract ( 30 µg ) using CD36 polyclonal antibody ( Cat # PAB12463 ). Lane 1 : 0.5 µg/ml ; lane 2 : 2 µg/ml.
Detection of CD36 in human adipocyte extract ( 30 µg ) using CD36 polyclonal antibody ( Cat # PAB12464 ). Lane 1 : 0.5 µg/ml ; lane 2 : 2 µg/ml.
Surface staining of human platelets with CD36 monoclonal antibody, clone TR9 (FITC) ( Cat # MAB4415 ).
Surface staining of human platelets with anti-CD36 (TR9) FITC.
Mouse Gut; CD36 antibody - N-terminal region (ARP48127_P050) in Mouse Gut cells using Immunohistochemistry
Hum. Fetal Heart; Host: Rabbit . Target Name: CD36 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; CD36 antibody - N-terminal region (ARP48127_P050) in Hum. Fetal Heart cells using Western Blot
Human Liver; WB Suggested Anti-CD36 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; CD36 antibody - N-terminal region (ARP48127_P050) in Human Liver cells using Western Blot
Human Fetal Liver; Host: Rabbit. Target Name:CD36. Sample Tissue:Human Fetal Liver. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12.; CD36 antibody - N-terminal region (ARP48127_P050) in Human Fetal Liver cells using Western Blot
Representative Standard Curve
Representative Standard Curve
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500921, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500921, Dilution 1:50)
Anti-CD36 mouse monoclonal antibody (TA500921) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD36(RC203254).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD36 (RC203254, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36.
Anti-CD36 mouse monoclonal antibody (TA500935) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD36(RC203254).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD36 (RC203254, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-CD36 monoclonal antibody.(1:200)
Flow cytometric Analysis of Hela cells, using anti-CD36 antibody(TA500935),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-CD36 antibody(TA500935),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500954, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500954, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500954, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD36 (RC203254, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506366)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506366)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506366)
Immunofluorescent staining of HeLa cells using anti-CD36 mouse monoclonal antibody (TA506366).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD36 (RC203254, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36.
CD36 ELISA with 3F4 Capture (TA600031) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
CD36 Luminex ELISA with 3F4 Capture (TA600031) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
CD36 ELISA with 6A5 Capture (TA600044) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
CD36 Luminex ELISA with 6A5 Capture (TA600044) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
CD36 ELISA with 3F4 Capture (TA600031) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
CD36 Luminex ELISA with 3F4 Capture (TA600031) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
CD36 Luminex ELISA with 6A5 Capture (TA600044) and 4H7 Detection (TA700031) Antibodies. Substrate used: Recombinant Human CD36 (TP303254)
Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml anti-CD36; lane 2: 2 ug/ml anti-CD36. ECL: 3 second exposure.
Staining of humnan adipocytes
Immunocytochemistry/Immunofluorescence: CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml anti-CD36; lane 2: 2 ug/ml anti-CD36. ECL: 3 second exposure.
Detection of CD36 in mouse adipose lysate
Staining of CD36 in mouse liver
Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD36 Purified (filled histogram) followed by Anti-Mouse IgM PE . Cells in the monocyte gate were used for analysis.
Staining of J774.1 cells with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD36 Purified (filled histogram) followed by Anti-Rat IgG PE . Total viable cells were used for analysis.
Western blot analysis of extracts of various cell lines, using CD36 antibody.
Western Blot: CD36 Antibody [TA337173] - Analysis of CD36 in human platelets using this antibody at 1:200.
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with purified HM36, followed by anti-Armenian hamester IgG FITC
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human placenta tissue, Human fat tissue, Primary antibody: (CD36 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CD36 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| SM1769A | CD36 antibody |
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Mouse | IgM | SM0 | Azide Free | Hu | C, F, FN, WB |
1 mg /
€810.00
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| Acris Antibodies GmbH | |||||||
Published recently! |
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| TA500921 | CD36 antibody |
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| anti-CD36 mouse monoclonal antibody, clone OTI3F4 (formerly 3F4) | |||||||
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Mouse | IgG1 | OTI3F4 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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| TA500935 | CD36 antibody |
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| CD36 mouse monoclonal antibody, clone OTI4H7 (formerly 4H7) | |||||||
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Mouse | IgG1 | OTI4H7 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
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| TA500954 | CD36 antibody |
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| anti-CD36 mouse monoclonal antibody, clone OTI6A5 (formerly 6A5) | |||||||
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Mouse | IgG1 | OTI6A5 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA506366 | CD36 antibody |
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| CD36 mouse monoclonal antibody, clone OTI1B3 (formerly 1B3) | |||||||
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Mouse | IgG1 | OTI1B3 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA301400 | CD36 antibody |
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| Rabbit Polyclonal Antibody against CD36 | |||||||
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Rabbit | IgG | Purified | Bov, Hu, Mky | C, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA301401 | CD36 antibody |
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| Rabbit Polyclonal Antibody against CD36 | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA301402 | CD36 antibody |
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| Mouse Monoclonal Antibody against CD36 (D-2712) | |||||||
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Mouse | IgA | D-2712 | Purified | Ms, Rt | IP, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA320264 | CD36 antibody |
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| Mouse Anti-Human CD36 Purified (100 ug) | |||||||
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Mouse | eBioNL07 (NL07) | Purified | Hu | F |
0.1 mg /
€180.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA332917 | CD36 antibody |
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| Rabbit anti-CD36 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA337173 | CD36 antibody |
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| Rabbit Polyclonal CD36/SR-B3 Antibody | |||||||
![Western Blot: CD36 Antibody [TA337173] - Analysis of CD36 in human platelets using this antibody at 1:200.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-27414-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.15 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA351048 | CD36 antibody |
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| Rabbit Polyclonal Anti-CD36 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA352306 | CD36 antibody |
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| Hamster Monoclonal anti-CD36 Antibody | |||||||
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Hamster | IgG | HM36 | Purified | Ms | F |
0.5 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA600031 | CD36 antibody |
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| ELISA and Luminex validated CD36 Mouse monoclonal antibody clone (3F4, clone OTI3F4 (formerly 3F4) | |||||||
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Mouse | IgG1 | OTI3F4 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA600044 | CD36 antibody |
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| ELISA and Luminex validated CD36 Mouse monoclonal antibody clone (6A5, clone OTI6A5 (formerly 6A5) | |||||||
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Mouse | IgG1 | OTI6A5 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA700031 | CD36 antibody |
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| ELISA and Luminex validated biotinylated CD36 Mouse monoclonal antibody clone (4H7), clone OTI4H7 (formerly 4H7) | |||||||
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Mouse | IgG1 | OTI4H7 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM03058AC-N | CD36 antibody |
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Mouse | IgG1 | TR9 | APC | Hu | F |
100 Tests /
€290.00
|
| Acris Antibodies GmbH | |||||||
| AM03058FC-N | CD36 antibody |
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Mouse | IgG1 | TR9 | FITC | Hu | F |
100 Tests /
€260.00
|
| Acris Antibodies GmbH | |||||||
| AM03058PU-N | CD36 antibody |
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Mouse | IgG1 | TR9 | Purified | Hu | F |
0.1 mg /
€210.00
|
| Acris Antibodies GmbH | |||||||
| AM03058RP-N | CD36 antibody |
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Mouse | IgG1 | TR9 | PE | Hu | F |
100 Tests /
€290.00
|
| Acris Antibodies GmbH | |||||||
