CD4 antibody

Principal name

CD4 antibody

Alternative names for CD4 antibody

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

SwissProt ID

A7YY52 (Bovin), F6Y6X8, P01730 (Human), P05540 (Rat), P05542 (Sheep), P06332 (Mouse), P33705 (Canfa), P46630 (Rabit), P79355 (Felca), Q6Q4G0 (Chick), Q6R3N4

Gene ID

24932 (Cd4), 12504, 920 (CD4), 404704, 407098, 403931, 493775, 100009152

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fe (Feline), Gt (Goat), Sh (Sheep), Rb (Rabbit), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD4 antibody

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin 
Intracellular ligands: p56Lck 
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

General readings

1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249. 
2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. 
3. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. 
4. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. 
5. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. 
6. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule. Poult Sci. 1999;78:1526-31. 
7. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. 
8. Koci MD, Kelley LA, Larsen D, Schultz-Cherry S. Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection. J Virol. 2004;78:1564-74. 
9. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. 
10. Tykałowski B, Stenzel T, Mikulski D, Jankowski J, Zduńczyk Z, Juśkiewicz J, et al. Level of electrolytes and percentage of T-lymphocyte subpopulations in blood of broiler chickens fed mixtures with different contents of sodium chloride. Bull Vet Inst Pulawy. 2011;55:333-7. 
11. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. 
12. Young JA, Jefferies W. Towards the conservation of endangered avian species a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One. 2013;8(6):e67137. 
13. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. 
14. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. 
15. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. 
16. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. 
17. Kjærup RM, Dalgaard TS, Norup LR, Hamzic E, Sørensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42. 
18. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. 
19. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI10B5 (formerly 10B5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Mouse IgG1 OTI10B5 Purified Can, Hu, Mky, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody,clone UMAB64  
CD4 Mouse IgG2b UMAB64 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD4 antibody

  CD4 mouse monoclonal antibody,clone UMAB64  
CD4 Mouse IgG2b UMAB64 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD4 antibody

Immunohistochemistry on frozen sections: Staining with AM26053PU-N on human tonsil Mouse IgG2a EDU-2 Aff - Purified Hu C, F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497F. Mouse IgG1 CVS4 FITC Eq F
0.1 mg / €440.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497PS followed by FITC conjugated secondary antibody. Mouse IgG1 CVS4 Purified Eq C, F, IP
0.1 mg / €380.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 antibody clone CVS4 Cat.-No SM497R. Mouse IgG1 CVS4 PE Eq F
100 Tests / €500.00
  Acris Antibodies GmbH

CD4 antibody

Figure 1. Immunoprecipitation of Human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-Human CD4 antibody (MEM-241). 
Lane 1: original lysate of T cells. 
Lane 2: immunoprecipitate by Negative Control antibody. 
Lane 3: immunoprecipitate by anti-Human CD4 antibody (MEM-115). Mouse IgG2a MEM-115 Azide Free Hu F, FN, IP
0.1 mg / €210.00
  Acris Antibodies GmbH

CD4 antibody

  Rabbit Polyclonal CD4 Antibody  
Western Blot: CD4 Antibody [TA336736] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta. Rabbit Purified Hu, Ms, Rb, Rt C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI4A11 (formerly 4A11)  
Anti-CD4 mouse monoclonal antibody (TA500479) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453). Mouse IgG2a OTI4A11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI18E3 (formerly 18E3)  
CD4 Mouse IgG2b OTI18E3 Purified Hu, Rt F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800512) Mouse IgG2a OTI5D9 Purified Hu, Mky P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI2H8 (formerly 2H8)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800524) Mouse IgG2a OTI2H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTIG9 (formerly G9)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802064) Mouse IgG1 OTIG9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTIC2 (formerly C2)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802067) Mouse IgG1 OTIC2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTIE11 (formerly E11)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802127) Mouse IgG1 OTIE11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802200) Mouse IgG1 OTI1G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802767) Mouse IgG1 OTI1D6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 (50-235) antibody

CD4 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
20 µl / €50.00
  SICGEN

CD4 (50-235) antibody

CD4 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €300.00
  SICGEN

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