CD4 antibody

Principal name

CD4 antibody

Alternative names for CD4 antibody

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

SwissProt ID

A7YY52 (Bovin), F6Y6X8, P01730 (Human), P05540 (Rat), P05542 (Sheep), P06332 (Mouse), P33705 (Canfa), P46630 (Rabit), P79355 (Felca), Q6Q4G0 (Chick), Q6R3N4

Gene ID

24932 (Cd4), 12504, 920 (CD4), 404704, 407098, 403931, 493775, 100009152, 395362

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fe (Feline), Gt (Goat), Sh (Sheep), Rb (Rabbit), Tr (Turkey), Chimp (Chimpanzee), GP (Guinea Pig), Duck

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD4 antibody

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin 
Intracellular ligands: p56Lck 
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

General readings

1. Maddox, J. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies. Immunology. 55: 739 - 748.
2. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol. 16: 19 - 25.
3. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 - 55.
4. Mackay, C. et al. (1986) Thymocyte subpopulations during early fetal development in sheep. J. Immunol. 136 (5): 1592 - 1599.
5. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils. Acta Histochem. May 21. [Epub ahead of print]
6. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 42: 37.
7. Brown, M.N. et al. (2010) Chemoattractant receptors and lymphocyte egress from extralymphoid tissue: changing requirements during the course of inflammation. J Immunol. 185: 4873-82.
8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes. Immunology. 107: 366-72.
9. Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression. J Immunol Methods. 339: 1-10.
10. Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues. Nat Immunol. 6: 889-94.
11. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis. Clin Vaccine Immunol. 17: 108-17.
12. Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models. J Virol. Dec 7. [Epub ahead of print].
13. Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD4 antibody

CD4 Mouse IgG1 OTI10B5 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

CD4 Mouse IgG2b UMAB64 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD4 antibody

CD4 Mouse IgG2b UMAB64 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD4 antibody

Immunohistochemistry on frozen sections: Staining with AM26053PU-N on human tonsil Mouse IgG2a EDU-2 Aff - Purified Hu C, F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497F. Mouse IgG1 CVS4 FITC Eq F
0.1 mg / €440.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497PS followed by FITC conjugated secondary antibody. Mouse IgG1 CVS4 Purified Eq C, F, IP
0.1 mg / €380.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 antibody clone CVS4 Cat.-No SM497R. Mouse IgG1 CVS4 PE Eq F
100 Tests / €500.00
  Acris Antibodies GmbH

CD4 antibody

Figure 1. Immunoprecipitation of Human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-Human CD4 antibody (MEM-241). 
Lane 1: original lysate of T cells. 
Lane 2: immunoprecipitate by Negative Control antibody. 
Lane 3: immunoprecipitate by anti-Human CD4 antibody (MEM-115). Mouse IgG2a MEM-115 Azide Free Hu F, FN, IP
0.1 mg / €210.00
  Acris Antibodies GmbH

CD4 antibody

Western Blot: CD4 Antibody [TA336736] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta. Rabbit Purified Hu, Ms, Rb, Rt C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD4 antibody

Anti-CD4 mouse monoclonal antibody (TA500479) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453). Mouse IgG2a OTI4A11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

CD4 Mouse IgG2b OTI18E3 Purified Hu, Rt F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800512) Mouse IgG2a OTI5D9 Purified Hu, Mky P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800524) Mouse IgG2a OTI2H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802064) Mouse IgG1 OTIG9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802067) Mouse IgG1 OTIC2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802127) Mouse IgG1 OTIE11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802200) Mouse IgG1 OTI1G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802767) Mouse IgG1 OTI1D6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Human tonsil stained with  Anti-CD4 Antibody Cat.-No AM11105PU (Clone SP35). Rabbit SP35 Purified Hu F, P
0.5 ml / €540.00
  Acris Antibodies GmbH

CD4 antibody

Human tonsil stained with  Anti-CD4 Antibody Cat.-No AM11105PU (Clone SP35). Rabbit SP35 Purified Hu F, P
1 ml / €780.00
  Acris Antibodies GmbH

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