CD4 antibody

Principal name

CD4 antibody

Alternative names for CD4 antibody

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

SwissProt ID

A7YY52 (Bovin), F6Y6X8, P01730 (Human), P05540 (Rat), P05542 (Sheep), P06332 (Mouse), P33705 (Canfa), P46630 (Rabit), P79355 (Felca), Q6Q4G0 (Chick), Q6R3N4

Gene ID

24932 (Cd4), 12504, 920 (CD4), 404704, 407098, 403931, 493775, 100009152

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fe (Feline), Gt (Goat), Sh (Sheep), Rb (Rabbit), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD4 antibody

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin 
Intracellular ligands: p56Lck 
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

General readings

1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249. 
2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. 
3. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. 
4. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. 
5. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. 
6. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule. Poult Sci. 1999;78:1526-31. 
7. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. 
8. Koci MD, Kelley LA, Larsen D, Schultz-Cherry S. Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection. J Virol. 2004;78:1564-74. 
9. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. 
10. Tykałowski B, Stenzel T, Mikulski D, Jankowski J, Zduńczyk Z, Juśkiewicz J, et al. Level of electrolytes and percentage of T-lymphocyte subpopulations in blood of broiler chickens fed mixtures with different contents of sodium chloride. Bull Vet Inst Pulawy. 2011;55:333-7. 
11. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. 
12. Young JA, Jefferies W. Towards the conservation of endangered avian species a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One. 2013;8(6):e67137. 
13. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. 
14. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. 
15. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. 
16. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. 
17. Kjærup RM, Dalgaard TS, Norup LR, Hamzic E, Sørensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42. 
18. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. 
19. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33.

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CRISPR

Catalog No. Species Donor Vector  

Cd4 - mouse gene knockout kit via CRISPR

Cd4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cd4 - mouse gene knockout kit via CRISPR

Cd4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cd4 - mouse gene knockout kit via CRISPR

Cd4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cd4 - mouse gene knockout kit via CRISPR

Cd4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

4 Item(s)

per page
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