CD4 antibody

Principal name

CD4 antibody

Alternative names for CD4 antibody

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

SwissProt ID

A7YY52 (Bovin), F6Y6X8, P01730 (Human), P05540 (Rat), P05542 (Sheep), P06332 (Mouse), P33705 (Canfa), P46630 (Rabit), P79355 (Felca), Q6Q4G0 (Chick), Q6R3N4

Gene ID

24932 (Cd4), 12504, 920 (CD4), 404704, 407098, 403931, 493775, 100009152

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fe (Feline), Gt (Goat), Sh (Sheep), Rb (Rabbit), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD4 antibody

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin 
Intracellular ligands: p56Lck 
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

General readings

1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249. 
2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. 
3. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. 
4. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. 
5. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. 
6. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule. Poult Sci. 1999;78:1526-31. 
7. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. 
8. Koci MD, Kelley LA, Larsen D, Schultz-Cherry S. Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection. J Virol. 2004;78:1564-74. 
9. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. 
10. Tykałowski B, Stenzel T, Mikulski D, Jankowski J, Zduńczyk Z, Juśkiewicz J, et al. Level of electrolytes and percentage of T-lymphocyte subpopulations in blood of broiler chickens fed mixtures with different contents of sodium chloride. Bull Vet Inst Pulawy. 2011;55:333-7. 
11. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. 
12. Young JA, Jefferies W. Towards the conservation of endangered avian species a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One. 2013;8(6):e67137. 
13. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. 
14. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. 
15. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. 
16. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. 
17. Kjærup RM, Dalgaard TS, Norup LR, Hamzic E, Sørensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42. 
18. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. 
19. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497F. Mouse IgG1 CVS4 FITC Eq F
0.1 mg / €440.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497PS followed by FITC conjugated secondary antibody. Mouse IgG1 CVS4 Purified Eq C, F, IP
0.1 mg / €380.00
  Acris Antibodies GmbH

CD4 antibody

Horse peripheral blood lymphocytes stained with mouse anti horse CD4 antibody clone CVS4 Cat.-No SM497R. Mouse IgG1 CVS4 PE Eq F
100 Tests / €500.00
  Acris Antibodies GmbH

CD4 antibody

Figure 1. Immunoprecipitation of Human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-Human CD4 antibody (MEM-241). 
Lane 1: original lysate of T cells. 
Lane 2: immunoprecipitate by Negative Control antibody. 
Lane 3: immunoprecipitate by anti-Human CD4 antibody (MEM-115). Mouse IgG2a MEM-115 Azide Free Hu F, FN, IP
0.1 mg / €210.00
  Acris Antibodies GmbH

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI18E3 (formerly 18E3)  
CD4 Mouse IgG2b OTI18E3 Purified Hu, Rt F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

Profile of human whole blood analyzed by the BD FACSCanto II. Staining of blood cells with anti-human CD4 FITC (blue, 10 μl per test). Rabbit IgG A19-I FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD4 antibody

Flow Cytometric analysis of blood T cells using CD4 antibody Cat.-No AM06077PU-N (M2) and Negative Control (M1). Mouse IgG1 B486A1 Purified Hu E
0.1 ml / €280.00
  Acris Antibodies GmbH

CD4 antibody

Immunofluorescent Staining: Peripheral blood mononuclear cells from a healthy domestic Cat were double-stained with Mouse anti-Feline CD4-FITC and Mouse anti-Feline CD8-PE. Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScan(TM) flow cytometry (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG1 3-4F4 FITC Fe F
0.5 mg / €450.00
  Acris Antibodies GmbH

CD4 antibody

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD4-PE and Mouse anti-Chicken CD3-FITC (AM08114FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.1 µg/10e6 cells. Mouse IgG1 CT-4 PE Chk F
0.1 mg / €320.00
  Acris Antibodies GmbH

CD4 antibody

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with anti-Chicken CD4-FITC and anti-Chicken TCR alpha/beta-PE (AM08114FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgM EP96 FITC Chk F
0.5 mg / €450.00
  Acris Antibodies GmbH

CD4 antibody

Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC. Rat IgG2b GK1.5 APC Ms F
0.1 mg / €290.00
  Acris Antibodies GmbH

CD4 antibody

Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC. Rat IgG2b GK1.5 Azide Free Ms C, F, FN, ICC/IF, IP
0.1 mg / €160.00
  Acris Antibodies GmbH

CD4 antibody

Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC. Rat IgG2b GK1.5 FITC Ms F
0.1 mg / €180.00
  Acris Antibodies GmbH

CD4 antibody

Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC. Rat IgG2b GK1.5 Low Endotoxin Ms C, F, FN, ICC/IF, IP
0.1 mg / €160.00
  Acris Antibodies GmbH

CD4 antibody

Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC. Rat IgG2b GK1.5 PE Ms F
0.1 mg / €200.00
  Acris Antibodies GmbH

CD4 antibody

Flow cytometric analysis of CD4 antigen expression on human lymphocyte (left) and human monocyte (right). Open histograms indicate the reaction of Isotypic control to the cells. Shaded histograms indicate the reaction of AM26438AF-N to the cells. Mouse IgG1 4H5 Azide Free Hu F
0.1 mg / €390.00
  Acris Antibodies GmbH

CD4 antibody

A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes Mouse IgG2a B-A1 Azide Free Hu F, FN, P
0.2 mg / €270.00
  Acris Antibodies GmbH

CD4 antibody

A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes Mouse IgG2a B-A1 FITC Hu F
1ml (100 Tests) / €270.00
  Acris Antibodies GmbH

CD4 antibody

A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes Mouse IgG2a B-A1 Purified Hu F, P
2ml (200 Tests) / €250.00
  Acris Antibodies GmbH

CD4 antibody

A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes Mouse IgG2a B-A1 PE Hu F
1ml (100 Tests) / €290.00
  Acris Antibodies GmbH

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