CD4 antibody

Principal name

CD4 antibody

Alternative names for CD4 antibody

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

SwissProt ID

A7YY52 (Bovin), F6Y6X8, P01730 (Human), P05540 (Rat), P05542 (Sheep), P06332 (Mouse), P33705 (Canfa), P46630 (Rabit), P79355 (Felca), Q6Q4G0 (Chick), Q6R3N4

Gene ID

24932 (Cd4), 12504, 920 (CD4), 404704, 407098, 403931, 493775, 100009152

Available reactivities

Rt (Rat), Ms (Mouse), Hu (Human), Mky (Monkey), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fe (Feline), Gt (Goat), Sh (Sheep), Rb (Rabbit), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD4 antibody

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin 
Intracellular ligands: p56Lck 
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

General readings

1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249. 
2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. 
3. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. 
4. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. 
5. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. 
6. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule. Poult Sci. 1999;78:1526-31. 
7. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. 
8. Koci MD, Kelley LA, Larsen D, Schultz-Cherry S. Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection. J Virol. 2004;78:1564-74. 
9. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. 
10. Tykałowski B, Stenzel T, Mikulski D, Jankowski J, Zduńczyk Z, Juśkiewicz J, et al. Level of electrolytes and percentage of T-lymphocyte subpopulations in blood of broiler chickens fed mixtures with different contents of sodium chloride. Bull Vet Inst Pulawy. 2011;55:333-7. 
11. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. 
12. Young JA, Jefferies W. Towards the conservation of endangered avian species a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One. 2013;8(6):e67137. 
13. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. 
14. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918. 
15. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80. 
16. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. 
17. Kjærup RM, Dalgaard TS, Norup LR, Hamzic E, Sørensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42. 
18. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92. 
19. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD4 antibody

  Rat Anti-Mouse CD4 Purified (100 ug)  
Staining of C57Bl/6 splenocytes with 0.06 ug of Rat IgG2b Isotype Control Purified (open histogram) or 0.06 ug of Anti-Mouse CD4 Purified (filled histogram) followed by Anti-Rat IgG FITC .  Total viable cells were used for analysis. Rat GK1.5 Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD4 antibody

  Rat Anti-Mouse CD4 Purified (100 ug)  
Staining of C57Bl/6 thymocytes with 0.25 ug of Rat IgG2b Isotype Control Purified (open histogram) or 0.25 ug of  Anti-Mouse CD4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Cells in the lymphocyte gate were used for analysis. Rat RM4-4 Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD4 antibody

  Rat Anti-Mouse CD4 Purified (100 ug)  
Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD4 Purified  (filled histogram) followed by Anti-Rat IgG FITC .  Total viable cells were used for analysis. Rat RM4-5 Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD4 antibody

  Mouse Anti-Human CD4 Purified (100 ug)  
Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD4 Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Cells in the lymphocyte gate were used for analysis. Mouse RPA-T4 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD4 antibody

  Mouse Monoclonal CD4 Antibody (B486A1)  
Western Blot: CD4 Antibody (B486A1) [TA336281] - Western blot analysis of human tonsil tissue using CD4 antibody at a concentration of 2 ug/ml. Mouse IgG1 B486A1 Purified Hu C, E, F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Goat Polyclonal Anti-CD4 Antibody  
TA348948 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Mouse Monoclonal Anti-CD4 Antibody [9H5A8]  
Western blot analysis of CD4 in human thymus tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 ug/mL. Mouse IgG 9H5A8 Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Mouse Monoclonal Anti-CD4 Antibody [8G1B12]  
Western blot analysis of CD4 in rat lung tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 ug/mL. Mouse IgG 8G1B12 Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Rat Monoclonal anti-CD4 Antibody  
C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, kappa; isotype control (open histogram). Rat RM4-5 Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI9C1 (formerly 9C1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Mouse IgG1 OTI9C1 Purified Can, Hu, Mky, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  anti-CD4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Mouse IgG1 OTI9B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  anti-CD4 mouse monoclonal antibody, clone OTI6E10 (formerly 6E10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD4 mouse monoclonal antibody. (TA500481) Mouse IgG1 OTI6E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  CD4 mouse monoclonal antibody, clone OTI3B4 (formerly 3B4)  
Anti-CD4 mouse monoclonal antibody (TA500482) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453). Mouse IgG2b OTI3B4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  anti-CD4 mouse monoclonal antibody, clone OTI9G7 (formerly 9G7)  
Anti-CD4 mouse monoclonal antibody (TA500484) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453). Mouse IgG1 OTI9G7 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  anti-CD4 mouse monoclonal antibody, clone OTI4F8 (formerly 4F8)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (TA500488)(1:150) Mouse IgG2b OTI4F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD4 antibody

  Rabbit Polyclonal CD4 Antibody  
CD4 Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Rabbit Polyclonal CD4 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Rabbit Polyclonal CD4 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Rabbit IgG Purified Hu, Mky, Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Luminex validated CD4 mouse monoclonal antibody (clone 6B7, clone OTI6B7 (formerly 6B7)  
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 2D4 Detection (TA700277) Antibodies. Substrate used: Recombinant Human CD4 (TP306453) Mouse IgG1 OTI6B7 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD4 antibody

  Luminex validated CD4 mouse monoclonal antibody (clone 7F2, clone OTI7F2 (formerly 7F2)  
CD4 Luminex Elisa with 7F2 Capture (TA600278) and 4A9 Detection (TA700279) Antibodies. Substrate used: Recombinant Human CD4 (TP306453) Mouse IgG1 OTI7F2 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

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