CD40 antibody

Principal name

CD40 antibody

Alternative names for CD40 antibody

TNFRSF5, CDw40, Bp50, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, CD40L receptor

SwissProt ID

P25942 (Human), P27512 (Mouse), Q28203 (Bovin), Q3ZTK5 (Horse), Q7YRL5 (Canfa), Q8SQ34 (Pig)

Gene ID

958 (CD40), 21939 (Cd40)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), GP (Guinea Pig), Hst (Hamster), Rb (Rabbit), Sh (Sheep), Chk (Chicken), Bov (Bovine)

Available hosts

Mouse, Rabbit, Rat, Goat, Hamster

Available applications

Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunohistochemistry (IHC)

Background of CD40 antibody

CD40 is a transmembrane glycoprotein mainly expressed on the surface of B cells and is also expressed on monocytes, dendritic cells, endothelial cells, and epithelial cells. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 ligand (CD40L, CD154, gp39, TRAM) belongs to the TNF gene family and is expressed more widely than activated CD4+ T cells. Following interaction with CD40L, CD40 mediates a number of major immunoregulatory functions, is central to the control of thymus-dependent humoral immunity and may be critical in the development of cell-mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphoma and to induce the death of transformed cells of mesenchymal or epithelial origin. In their resting state up to 50% of B cells may express CD40L in their cytoplasm, but not on the surface and this cytoplasmic CD40L is readily released as a soluble receptor. The proportion of expressing cells and the amount of CD40L expressed is increased by signaling through immunoglobulin (Ig) or CD38. The signal transduction pathways triggered through CD40 have not yet been fully delineated. Early biochemical events include activation of Ras, of Lyn and Syk protein tyrosine kinases, phosphorylation and activation of phospholipase C2 and phosphoinositide 3 kinase, and induction of mitogen-activated protein kinases. Activation processes seem to be initiated by association of the CD40 receptor with signaling molecules of the TRAF family.

General readings

1. Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions. J Exp Med. 1996 May 1;183(5):2209-18.
2. Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106.
3. Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of
mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13(3):273-83.
4. Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD40 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-CD40 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800075)(1:200) Mouse IgG1 UMAB183 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-CD40 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800075)(1:200) Mouse IgG1 UMAB183 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807569) Mouse IgG2a OTI8B8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807575) Mouse IgG1 OTI1F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807682) Mouse IgG1 OTI9D8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807683)(1:150) Mouse IgG2b OTI5C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807686)(1:150) Mouse IgG2b OTI9F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

IHC staining on Bouin paraffin lymph node section. Human CD40 stained with mAb 89. Mouse IgG1 mAb89 Purified Hu C, F, FN, P
50 µg / €240.00
  Dendritics SAS

CD40 antibody

IHC staining on Bouin paraffin lymph node section. Human CD40 stained with mAb 89. Mouse IgG1 MAB89 Purified Hu C, F, FN, P
0.1 mg / €310.00
  Dendritics SAS

CD40 (C-term) antibody

Human Hodgkin’s lymphoma stained with Anti-CD40 antibody Rabbit Purified Bov, Can, Hu, Ms, Por P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD40 (C-term) antibody

Human Hodgkin’s lymphoma stained with Anti-CD40 antibody Rabbit Purified Bov, Can, Hu, Ms, Por P
1 ml / €440.00
  Acris Antibodies GmbH

CD40 (C-term) antibody

Human Hodgkin’s lymphoma stained with Anti-CD40 antibody Rabbit Purified Bov, Can, Hu, Ms, Por P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD40 (Isoform 1) antibody

AP33488PU-N(1µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD40 antibody

Western blot analysis of extracts from COS7 cells, using CD40 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD40 antibody

Western blot analysis of CD40 expression in rat kidney tissue lysate. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD40 antibody

CD40 Mouse 5C3 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD40 antibody

Staining of BALB/c splenocytes with 0.5 ug Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.5 ug Anti-Mouse/Rat CD40 Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC .  Total viable cells were used for analysis. Hamster HM40-3 Purified Ms, Rt F, P
0.1 mg / €150.00
  OriGene Technologies, Inc.

CD40 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40. Mouse IgG1 OTI7H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807572)(1:150) Mouse IgG1 OTI8G5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD40 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40. Mouse IgG2b OTI8C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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