CD43 / Leukosialin antibody

Principal name

CD43 / Leukosialin antibody

Alternative names for CD43 / Leukosialin antibody

Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN

SwissProt ID

P13838 (Rat), P15702 (Mouse), P16150 (Human)

Gene ID

6693 (SPN), 20737 (Spn), 9332 (CD163)

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Rat

Available applications

Flow Cytometry (F), Functional assay (FN), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of CD43 / Leukosialin antibody

Human CD43 antigen is a heavily sialylated single chain membrane glycoprotein with both molecular weight and antigenicity depending on the nature and complexity of the attached O-linked polysaccharides. Human CD43 is an integral membrane protein with a 235 amino acids (a.a.) extracellular domain, a transmembrane domain of 23 a.a and a 123 a.a. intracytoplasmic domain. The extracellular domain of CD43 has been shown to react with many ligands such as ICAM-1(CD54), E-selectin, galectin-1, MHC-I, and human serum albumin (HSA). The intracytoplasmic domain, which is highly conserved between species, is constitutively phosphorylated on serine residues. The level of phosphorylation increases after cell activation. This cell surface glycoprotein is also called leukosialin, sialophron or GPL115. A soluble form of CD43 is also present in human serum. Thymocytes, CD4+ T lymphocytes and monocytes express more of a 115 kDa CD43 isoform, whereas a 130 kDa isoform is found mostly on activated CD4+ T cells, CD8+ resting and activated T cells, neutrophils, platelets, and B cells. CD43 plays a role in the regulation of lymphoid cell adhesion, cellular maturation, and activation although the nature of this participation remains controversial. For example, the structural characteristics of the CD43 extracellular domain indicate that this molecule has both adhesive and anti-adhesive properties. O-glycosylated chains of CD43 such as a sialyl-Lewis x (sLex) epitope, a ligand for both P- and E-selectin, potentiate binding to lectin-like molecules. However, the adhesion molecule function for CD43 is counteracted by the presence of negatively charged sialic acid residues on its extracellular domain. In addition to the strong structural evidence for the involvement of CD43 in cell interactions, experimental data suggest contradictive role for CD43 in regulating cell contacts.

General readings

1. D. Opstelten et al. B lymphocyte-associated antigens on terminal deoxynucleotidyl transferase-positive cells and pre-B cells in bone marrow of the rat. J. Immunol.137, 76, (1986)
2. F.G.M. Kroese et al. The ontogeny of germinal center forming capacity of neonatal rat spleen. Immunology, 60, 597, (1987)
3. F.G.M. Kroese et al. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage associated antigens. Eur. J. Immunol. 17, 921, (1987)
4. J. Kampinga et al. Thymocyte differentiation and thymic micro-environment development in the fetal rat thymus: an immunohistochemical approach. In: Thymus Update 3. The role of the thymus in tolerance induction. Eds. M.D. Kendall and M. A. Ritter. Harwood Academic Publishers GmbH, Switzerland (1990)
5. F.G.M. Kroese et al T. Lymphocytes in rat lymphoid follicles are a subset of T helper cells. Adv. Exp. Med. Biol. 186, 443, (1985)
OX-12

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD43 / Leukosialin antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SPN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800785) Mouse IgG1 OTI6A4 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD43 / Leukosialin antibody

Formalin-Paraffin Human Spleen stained with Cp43 Antibody Cat.-No AM08160PU (Clone DF-T1). Mouse IgG1 DF-T1 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Formalin-Paraffin Human Spleen stained with Cp43 Antibody Cat.-No AM08160PU (Clone DF-T1). Mouse IgG1 DF-T1 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Formalin-Paraffin Human Spleen stained with Cp43 Antibody Cat.-No AM08160PU (Clone DF-T1). Mouse IgG1 DF-T1 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Human Tonsil stained with anti-CD43 Antibody Cat.-No AM11106PU (Clone SP55). Rabbit SP55 Purified Hu F, P, WB
0.5 ml / €520.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Human Tonsil stained with anti-CD43 Antibody Cat.-No AM11106PU (Clone SP55). Rabbit SP55 Purified Hu F, P, WB
1 ml / €750.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Human Tonsil stained with anti-CD43 Antibody Cat.-No AM11106PU (Clone SP55). Rabbit SP55 Purified Hu F, P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Formalin-paraffin spleen stained with CD43 Antibody Cat.-No AM50171PU (Clone SPM503). Mouse IgG1 SPM503 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Formalin-paraffin spleen stained with CD43 Antibody Cat.-No AM50171PU (Clone SPM503). Mouse IgG1 SPM503 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Formalin-paraffin spleen stained with CD43 Antibody Cat.-No AM50171PU (Clone SPM503). Mouse IgG1 SPM503 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Tonsil stained with Anti-CD43 antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Tonsil stained with Anti-CD43 antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

Tonsil stained with Anti-CD43 antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD43 / Leukosialin antibody

CD43 / Leukosialin Mouse eBio84-3C1 (84-3C1) Purified Hu F, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD43 / Leukosialin antibody

Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE .  Cells in the lymphocyte gate were used for analysis. Rat eBioR2/60 Purified Ms F, IP, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD43 / Leukosialin antibody

Immunohistochemistry staining of Paraffin Tonsil tissue by CD43 antibody (dilution: 1:50 - 1:200; visualization of staining: Membranous) Mouse IgG1 MT1 Purified Hu P
1 ml / €440.00
  OriGene Technologies, Inc.

CD43 / Leukosialin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPN (RC204195, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPN. Mouse IgG1 OTID11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD43 / Leukosialin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPN (RC204195, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPN. Mouse IgG1 OTIF6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD43 / Leukosialin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPN (RC204195, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPN. Mouse IgG1 OTI2D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD43 / Leukosialin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SPN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800797) Mouse IgG1 OTI4G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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