CD45 / LCA antibody

Principal name

CD45 / LCA antibody

Alternative names for CD45 / LCA antibody

PTPRC, Leukocyte common antigen, L-CA, T200, Receptor-type tyrosine-protein phosphatase C

SwissProt ID

P04157 (Rat), P06800 (Mouse), P08575 (Human), Q6SZ85 (Pig), Q9GMB4 (Bovin), Q9I8D7 (Chick)

Gene ID

24699 (Ptprc), 5788 (PTPRC), 19264

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Prim (Primate), Bov (Bovine), Fe (Feline), Por (Porcine), Rb (Rabbit), Chk (Chicken), Mky (Monkey), Can (Canine), GP (Guinea Pig), Sh (Sheep), Gt (Goat), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit, Chicken, Goat

Available applications

Cytotoxicity Assay (CT), Functional assay (FN), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Radioimmunoassay (R)

Background of CD45 / LCA antibody

Ly 5 is a major cell surface component of T and B lymphocytes and most hematopoietic cells including promyelocytes, metamyelocytes, macrophages, eosinophils and neutrophils but is absent from mature erythroid cells (1). An interesting feature of the Ly 5 system is that members of this family of glycoproteins range in size from approximately 180 kDa to 240 kDa and differ in both protein sequence and carbohydrate content (2). Ly 5 alloantigens may be present in one of several molecular isoforms, and it has been postulated that each defines stages and lineages of hematopoietic differentiation (2). B lymphocytes express a 220 kDa form (B220) (3), while T lymphocytes indicate a more complicated pattern of expression with at least four forms identified by SDS-PAGE (2). Different patterns of expression have been identified between T lymphocyte subsets, indicating that expression of individual members is controlled in a cell-type specific fashion. Individual T cells may also express more than one member of the Ly 5 family and during activation, expression of the various forms may change (4). Additionally, there appears to be further Ly 5 modifications that occur as mature thymocytes migrate to peripheral lymphoid organs (4). Thus, the Ly 5 antigen exhibits inter and intratissue polymorphism, and it becomes clear that a programmed sequence of Ly 5 expression occurs during T cell differentiation and activation and that the forms expressed by lymphocyte populations are dynamic.
The Ly 5.2 antigen has also been defined as a major component of natural killer cells and it has been further suggested that the Ly 5 (or modified Ly 5) may be part of or close to a receptor on natural killer cells, for their target (6,7).

General readings

1. Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362(1):26-30.
2. Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.
3. Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
4. Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
5. Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
6. Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19.
7. Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34 1):23-34.
9. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
10. Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD45 / LCA (CD45RA) antibody

Formalin-Fixed, Paraffin-Embedded Human spleen stained with CDR5RA Antibody Cat.-No AM32858PU (Clone 158-4D3). Mouse IgG2a 158-4D3 Purified Hu C, F, ICC/IF, IP, P
0.2 mg / €390.00
  Acris Antibodies GmbH

CD45 / LCA (CD45RA) antibody

Formalin-Fixed, Paraffin-Embedded Human spleen stained with CDR5RA Antibody Cat.-No AM32858PU (Clone 158-4D3). Mouse IgG2a 158-4D3 Purified Hu C, F, ICC/IF, IP, P
0.1 mg / €320.00
  Acris Antibodies GmbH

CD45 / LCA (CD45RA) antibody

Formalin-Fixed, Paraffin-Embedded Human spleen stained with CDR5RA Antibody Cat.-No AM32858PU (Clone 158-4D3). Mouse IgG2a 158-4D3 Purified Hu C, F, ICC/IF, IP, P
20 µg / €160.00
  Acris Antibodies GmbH

CD45 / LCA (CD45RB) antibody

CD45 / LCA Rat IgG2a 16A PE Ms F
50 µg / €230.00
  Acris Antibodies GmbH

CD45 / LCA (CD45RB) antibody

CD45 / LCA Rat IgG2a 16A PE Ms F
0.3 mg / €540.00
  Acris Antibodies GmbH

CD45 / LCA antibody

Staining of mouse spleen cells with RAT ANTI MOUSE CD45 (Ly-5) Rat IgG2b YW 62.3 Purified Ms C, F, IP
0.25 mg / €390.00
  Acris Antibodies GmbH

CD45 / LCA antibody

Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD45 -RPE (Cat.-No SM274R). Mouse IgG1 OX-1 PE Rt F
100 Tests / €510.00
  Acris Antibodies GmbH

CD45 / LCA (CD45RB) antibody

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-CD45RB (MEM-55). Mouse IgG1 MEM-55 Purified Hu, Prim F, IP, P, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPRC mouse monoclonal antibody. (TA506046; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI2E7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PTPRC mouse monoclonal antibody. (TA506054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI1B7 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PTPRC mouse monoclonal antibody. (TA506055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI2H1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PTPRC mouse monoclonal antibody. (TA506056; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI2F1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506534) Mouse IgG1 OTI1F6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506537) Mouse IgG1 OTI1B6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunofluorescent staining of HeLa cells using anti-PTPRC mouse monoclonal antibody (TA506545). Mouse IgG1 OTI3E3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506559) Mouse IgG1 OTI3F2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPRC mouse monoclonal antibody. (TA506563; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI3D1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506566) Mouse IgG2b OTIC8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PTPRC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506567) Mouse IgG1 OTI4C11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD45 / LCA antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD45RO mouse monoclonal antibody. (TA807197; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG2a UCH-L1 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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