CD48 antibody

Principal name

CD48 antibody

Alternative names for CD48 antibody

BCM1, BLAST1, MEM-102, TCT.1, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102

SwissProt ID

P09326 (Human), P10252 (Rat), P18181 (Mouse)

Gene ID

962 (CD48), 12506 (Cd48), 245962

Available reactivities

Hu (Human), Prim (Primate), Ms (Mouse), Mky (Monkey), Rt (Rat)

Available hosts

Mouse, Rat, Rabbit, Hamster

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CD48 antibody

The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen; also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. Recent publications have reported differential expression of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors based on the expression of these SLAM family members. Hematopoietic stem cells (HSC) are highly purified as CD150(+)CD244(-)CD48(-) cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244(+)CD150(-)CD48(-) and the most restricted progenitors are CD48(+)CD244(+)CD150(-). CD48 plays a critical role in adhesion and T cell activation. In the mouse, the primary counter-receptors for CD48 are CD2 and CD244. HM48-1 is reported to modulate in vitro and in vivo CD48 functions including blocking the CD48/CD2 and CD48/CD244 interactions, inhibiting the proliferative response of mitogen-activated spleen cells, providing a costimulation signal for T cells activated in vitro through their TCR, and prolonging cardiac allograft survival in vivo.

General readings

1. Assarsson E, Kambayashi T, Persson CM, Chambers BJ, Ljunggren HG: 2B4/CD48-mediated regulation of lymphocyte activation and function. J Immunol. 2005 Aug 15;175(4):2045-9.
2. Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA: Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells. J Immunol. 2005 Jul 15;175(2):1005-13.
3. Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V: Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006 Apr 15;107(8):3181-8.
4. Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F: CD48 is an allergen and IL-3-induced activation molecule on eosinophils. J Immunol. 2006 Jul 1;177(1):77-83.
5. Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V.: Monoclonal antibodies against human
leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97.
6. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
7. Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V.: The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1. Immunogenetics. 1991;33(2):108-12.
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
8. Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
9. Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93(4):1437-9.
10. Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7.
11. Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD48 (Center) antibody

Western blot analysis of SLAMF2 in rat lung tissue lysate with SLAMF2 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD48 antibody

Staining of normal human peripheral blood mononuclear cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified  (open histogram) or 0.5 ug of Anti-Human CD48 Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Cells in the lymphocyte gate were used for analysis. Mouse eBio156-4H9 (156-4H9) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD48 antibody

Staining of BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD48 Purified  (filled histogram) followed by Anti-Armenian Hamster IgG FITC .  Total viable cells were used for analysis. Hamster HM48-1 Purified Ms F, IP, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD48 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3B8-2C2 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CD48 control peptide

CD48
50 µg / €160.00
  Acris Antibodies GmbH

CD48 antibody

CD48 Rat IgG2a OX-78 FITC Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD48 antibody

CD48 Rat IgG2a OX-78 FITC Ms F
25 µg / €190.00
  Acris Antibodies GmbH

CD48 antibody

Staining of Mouse spleen cells with Rat Anti Mouse CD48 Antibody Cat.-No SM036. Rat IgG2a OX-78 Purified Ms F
0.25 mg / €390.00
  Acris Antibodies GmbH

CD48 antibody

CD48 Rat IgG2a OX-78 Purified Ms F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD48 antibody

CD48 Rat IgG2a OX-78 PE Ms F
100 Tests / €290.00
  Acris Antibodies GmbH

CD48 antibody

CD48 Rat IgG2a OX-78 PE Ms F
25 Tests / €210.00
  Acris Antibodies GmbH

CD48 antibody

Peripheral Human lymphocytes stained with Mouse Anti Human CD48-APC (SM1121APC). Mouse IgG1 MEM-102 APC Hu, Mky F
100 Tests / €430.00
  Acris Antibodies GmbH

CD48 antibody

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD48-FITC (SM1121F/FT). Mouse IgG1 MEM-102 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD48 antibody

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD48-FITC (SM1121F/FT). Mouse IgG1 MEM-102 FITC Hu, Mky F
25 µg / €190.00
  Acris Antibodies GmbH

CD48 antibody

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD48 (SM1121P). Mouse IgG1 MEM-102 Purified Hu F, IP, P, WB
0.2 mg / €430.00
  Acris Antibodies GmbH

CD48 antibody

Surface staining of human peripheral blood with anti-CD48 (MEM-102) PE. Mouse IgG1 MEM-102 Purified Hu, Prim F, IP, P, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD48 antibody

SM1121R/RT staining of human peripheral blood lymphocytes. Mouse IgG1 MEM-102 PE Hu F
100 Tests / €470.00
  Acris Antibodies GmbH

CD48 antibody

SM1121R/RT staining of human peripheral blood lymphocytes. Mouse IgG1 MEM-102 PE Hu, Mky F
25 Tests / €220.00
  Acris Antibodies GmbH

CD48 antibody

CD48 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

CD48 antibody

CD48 MaxPab polyclonal antibody. Western Blot analysis of CD48 expression in human spleen. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

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