CD49d / ITGA4 antibody

Principal name

CD49d / ITGA4 antibody

Alternative names for CD49d / ITGA4 antibody

Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4, CD49 antigen-like family member D

SwissProt ID

P13612 (Human), Q00651 (Mouse)

Gene ID

16401 (Itga4), 3676 (ITGA4), 3680 (ITGA9)

Available reactivities

Ms (Mouse), Hu (Human), Bov (Bovine), Mky (Monkey), Por (Porcine), Rt (Rat), Gt (Goat), Rb (Rabbit), Prim (Primate), Chk (Chicken), Hst (Hamster), Eq (Equine)

Available hosts

Rat, Mouse, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Functional assay (FN), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Radioimmunoassay (R)

Background of CD49d / ITGA4 antibody

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

General readings

1. Issekutz, T.B. (1991). Inhibition of in vivo lymphocyte migration to inflammation and homing to lymphoid tissues by the TA-2 monoclonal antibody. A likely role for VLA-4 in vivo. J. Immunol. 147: 4178-4184.
2. Issekutz, T.B. and Wykretowicz, A. (1991). Effect of a new monoclonal antibody, TA-2 that inhibits lymphocyte adherence to cytokine stimulated endothelium in the rat.
J. Immunol. 147:109-116.
3. Yang, H. et al. (1995). Prolongation of rat islet&allograft survival by treatment with monoclonal antibodies against VLA-4 and LFA-1. Transplantation 60: 71-76.
4. Palecanda, A. et al. (1997) Rat Mast cell binds to the vascular cell adhesion molecule VCAM-1 and mucosal addresin cell adhesion molecule-1(MAdCAM-1).
J. Immunol. 158: 2904-2910.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD49d / ITGA4 antibody

Western blot analysis using ITGA4 antibody Cat.-No AM06742PU-N against human ITGA4 (AA: 926-978) recombinant protein (Expected MW is 31.7 kDa Mouse IgG1 5C4 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD49d / ITGA4 (C-term) antibody

Western blot analysis of Integrin alpha 4 in Jurkat cell lysate with Integrin alpha 4 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD49d / ITGA4 (Center) antibody

Western blot analysis of Integrin alpha 4 using (A) 25 and (B) 100 ng of recombinant Integrin alpha 4 with Integrin alpha 4 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD49d / ITGA4 (Center) antibody

Western blot analysis of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD49d / ITGA4 antibody

Staining of mouse splenocytes with Anti-Mouse CD49d PE.  Appropriate isotype controls were used  (open histogram). Total viable cells were used for analysis. Rat R1-2 Purified Ms F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD49d / ITGA4 antibody

Predicted band size: 70; 140; 180 kDa . Positive control: Jurkat and Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2 : Jurkat and Raji cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD49d / ITGA4 antibody

Western blot analysis of Integrin alpha4 expression in PMA treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD49d / ITGA4 antibody

Anti-ITGA4 / Integrin Alpha 4 antibody IHC of human thymus, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD49d / ITGA4 antibody

Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD49d / ITGA4 antibody

Western blot (WB) analysis of ITGA4 antibody (Cat.-No.: AP01224PU-N) in extracts from Jurkat cells treated with PMA 125ng/ml. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD49d / ITGA4 (C-term) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD49d / ITGA4 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD49d / ITGA4 (C-term) antibody

Immunohistochemical analysis of paraffin embedded Mammary cancer sections, staining ITGA4 in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD49d / ITGA4 Control Peptide

CD49d / ITGA4
50 µg / €160.00
  Acris Antibodies GmbH

CD49d / ITGA4 Control Peptide

CD49d / ITGA4
50 µg / €160.00
  Acris Antibodies GmbH

CD49d / ITGA4 Control Peptide

CD49d / ITGA4
50 µg / €160.00
  Acris Antibodies GmbH

CD49d / ITGA4 antibody

CD49d / ITGA4 Mouse IgG1 BU49 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD49d / ITGA4 antibody

CD49d / ITGA4 Mouse IgG1 Bu49 FITC Hu F
25 µg / €190.00
  Acris Antibodies GmbH

CD49d / ITGA4 antibody

CD49d / ITGA4 Mouse IgG1 Bu49 Purified Hu C, F, IP
25 µg / €180.00
  Acris Antibodies GmbH

CD49d / ITGA4 antibody

Cell Source: TK1 Cells; 
Percentage of cells stained above control: 99.3% Rat IgG2b R1-2 Ascites Ms C, F, IP
0.5 ml / €500.00
  Acris Antibodies GmbH

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