CD5 antibody
Principal name
CD5 antibody
Alternative names for CD5 antibody
CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
SwissProt ID
F6PYA8, O73906 (Chick), P06127 (Human), P13379 (Mouse), P19238 (Bovin), P51882 (Rat), Q07452 (Sheep), Q9GMA0
Ncbi ID
NP_031676.3, NP_055022.2, NP_062168.1, NP_776324.1, NP_990252.1, 9823, 9606, NP_055022, NP_031676, ABM83003.1, NM_014207, NM_007650, NM_019295
Available reactivities
Available hosts
Available applications
Cytotoxicity Assay (CT), Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P), ELISA (capture) (E(capture)), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Functional assay (FN), Luminex (LMNX)
Background of CD5 antibody
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative
disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some
autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of
CD5 expression in the expanded CD8+ human T cells.
General readings
1. Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19(5):849-55.
Raman C.: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26(1-3):255-63.
2. Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
3. Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L.: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9.
3. Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F.: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992 Mar 1;148(5):1287-93.
4. Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I.: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996 Jan;5(1):167-9.
Cytotoxicity Index
Tissue Distribution
Cytotoxic Index
Antibody Titration by Cytotoxicity Analysis
CYTOTOXICITY ANALYSIS - Cell Source
CYTOTOXICITY ANALYSIS - CELL SOURCE
CYTOTOXICITY ANALYSIS
FLOW CYTOMETRY ANALYSIS
Cytotoxic Index
FLOW CYTOMETRY ANALYSIS - Cell Source
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
FLOW CYTOMETRY ANALYSIS
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
FLOW CYTOMETRY ANALYSIS
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
FLOW CYTOMETRY ANALYSIS
CYTOTOXICITY ANALYSIS - Antibody Titration
CYTOTOXICITY ANALYSIS
FLOW CYTOMETRY ANALYSIS
Cytotoxic Index
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
FLOW CYTOMETRY ANALYSIS
FLOW CYTOMETRY ANALYSIS - Tissue Distribution
FLOW CYTOMETRY ANALYSIS
Staining of human peripheral blood lymphocytes with Mouse Anti Human CD5-APC
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD5
Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD5:BIOTIN (SM2174B).
Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD5 (SM2174P).
Staining of bovine peripheral blood lymphocytes with Mouse Anti Bovine CD5 (Cat.-No SM436P).
Figure 2. Flow Cytometry analysis -Dot plot.
Figure 1. Flow Cytometry analysis of Human Peripheral Blood Lymphocytes (PBL) stained with CRIS1/CD5 antibody (1 μg/ml). Histogram - gated on lymphocytes, overlay with isotypic control (Mouse IgG2a).
Figure 1. Peripheral blood lymphocytesstained with AM05171RP-N Antibody.
Staining of Rat lymph node cells with Mouse Anti Rat CD5: FITC (SM258FX).
Staining of porcine peripheral blood lymphocytes with mouse anti porcine CD5:FITC
Immunofluorescent Staining: Cells from BALB/c spleen were double-stained with Rat anti-Mouse CD5-R-PE and rat anti-mouse CD19-FITC (Clone 6D5), following which small lymphocytes were gated and analyzed using a FACScan(TM)flow cytometer (BDIS, San Jose, CA). Amount used: < / = 0.2 µg/10e6 cells.
Human tonsil stained with Anti-CD5 antibody
AM11112PU CD5 antibody staining of Human tonsil.
Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were first incubated with Mouse anti-Chicken CD5 and then counter-stained with goat anti-Mouse IgG-FITC. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
Formalin-fixed and paraffin-embedded human tonsil tissue ( 4 µm ) stained with CD5 monoclonal antibody, clone A25-G ( Cat # MAB1646 ) shows strong positive immunostaining of T-lymphocytes. Kindly performed and provided by Katarina Poliakova, MD and Lubomir Straka, MD, Ph. D. from Clinical Pathology Presov, Ltd., Presov, Slovakia.
Immunohistochemical staining of CD5 on formalin fixed, paraffin embedded human tonsil with CD5 polyclonal antibody.
Flow Cytometry analysis of human peripheral blood lymphocytes ( PBL ) stained with CD5 monoclonal antibody, clone CRIS1 ( Cat # MAB3840 ) ( dilution of purified antibody 1 µg/ml ).Fig. A : Histogram - gated on lymphocytes, overlay with isotypic control ( mouse IgG2a ).Fig. B : Dot plot.
Human COLO205; WB Suggested Anti-CD5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate; CD5 antibody - N-terminal region (ARP58440_P050) in Human COLO205 cells using Western Blot
Figure 1. Western blot (WB) analysis of CD5 antibody (Cat.-No.: AP20211PU-N) in extracts from 283 cells.
T-1367 CD5 (T cell antigen T1,Tp67), mouse anti human, clone CRIS-1 (purified monoclonal IgG2a, lyophilized)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Surface staining of normal Human peripheral blood cells with anti-Human CD5 (UCHT2) FITC conjugated. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 µm) stained with CD5 antibody (DB 027) shows positive immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 µm) stained with CD5 antibody (DB 027) shows positive immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 µm) stained with CD5 antibody (DB 027) shows positive immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia.
Formalin-Fixed, Paraffin-Embedded Human tonsil tissue (4 µm) stained with CD5 antibody (DB 027) shows positive immunostaining of T-lymphocytes. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia.
A typical staining pattern with the B-B8 monoclonal antibody of lymphocytes
A typical staining pattern with the B-B8 monoclonal antibody of lymphocytes
A typical staining pattern with the B-H7 monoclonal antibody of lymphocytes
A typical staining pattern with the B-B8 monoclonal antibody of lymphocytes
A typical staining pattern with the B-B8 monoclonal antibody of lymphocytes
Cell Source: Thymus
Tissue Distribution
Cell Source: Thymus. Percentage of cells stained above control: 97.9%
Staining with clone MCD5 (CD5) monoclonal antibody is illustrated by flow cytometry analysis of normal blood cells. Direct staining was performed using 10 µl of the FITC-conjugated antibody with 100 µl blood sample.Testing by flow cytometry using a 'lyse-wash'method on whole blood from healthy donors showed the following values expressed in terms of % of the total lymphocyte count:Product code: AM39009FC-N (anti-CD5 FITC)Mean % positive: 72,61S.D.: 5,10% CV: 7,03
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody Cat.-No AM32836SU (Clone C5/473) at 1/200 dilution using peroxidase-conjugate and DAB chromogen. Note membrane staining.
Staining of equine peripheral blood lymphocytes with mouse anti equine CD5 clone CVS5 Cat.-No. SM498PS, followed by secondary FITC-conjugated antibody
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody Cat.-No AM50190PU (Clone SPM546).
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody Cat.-No AM32836PU (Clone C5/473) .
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody Cat.-No AM50236PU (Clone CD5/54/F6)
Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody Cat.-No AM50237PU (Clone C5/473 + CD5/54/F6).
Representative Standard Curve
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD5 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD5 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD5 Antibody; followed by anti-mouse IgG PE.
Anti-CD5 mouse monoclonal antibody (TA501333) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5(RC206494).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA501333), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA501334), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501335, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501335, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501335, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501335, Dilution 1:50)
Anti-CD5 mouse monoclonal antibody (TA501335) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5(RC206494).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA501335), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA501335),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501376)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501376)
Anti-CD5 mouse monoclonal antibody (TA501376) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5(RC206494).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA501376),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA501376), and then analyzed by flow cytometry.
ELISA: CD5 Antibody
CD5 ELISA with 10H3 Capture (TA600464) and 5D4 Detection (TA700464) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 3A9 Capture (TA600465) and 9E9 Detection (TA700465) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 5D4 Capture (TA600466) and 10H3 Detection (TA700466) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 2G8 Capture (TA600467) and 9E9 Detection (TA700465) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 2G8 Capture (TA600467) and 2D8 Detection (TA700467) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 3A9 Detection (TA700468) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 10H3 Detection (TA700466) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 2G8 Detection (TA700475) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 5D4 Detection (TA700464) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 10H3 Capture (TA600464) and 5D4 Detection (TA700464) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 5D4 Detection (TA700464) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 3A9 Capture (TA600465) and 9E9 Detection (TA700465) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 2G8 Capture (TA600467) and 9E9 Detection (TA700465) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 10H3 Detection (TA700466) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 5D4 Capture (TA600466) and 10H3 Detection (TA700466) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 2G8 Capture (TA600467) and 2D8 Detection (TA700467) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 3A9 Detection (TA700468) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 ELISA with 6F7 Capture (TA600468) and 2G8 Detection (TA700475) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
CD5 Luminex ELISA with 6F7 Capture (TA600468) and 2G8 Detection (TA700475) Antibodies. Substrate used: Recombinant Human CD5 (TP306494)
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA801940), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA801940),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA801941), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA801941),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA801972), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA801972),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802083),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). Dilution: 1:50
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802401), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802401),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802402), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802402),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802403), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802403),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802404), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802404),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802406), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802406),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802408),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). Dilution: 1:50
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802409), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802409),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802410), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802410),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802411),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). Dilution: 1:50
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. Dil
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802415), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802415),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802417), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802417),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802418), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802418),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802422), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802422),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802423),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). Dilution: 1:50
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802424), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802424),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802425), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802425),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802427)
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802427), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802427),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802428), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802428),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802439), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802439),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells transfected with either RC206494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD5 antibody(TA802440), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(TA802440),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Staining of mouse splenocytes with Anti-Mouse CD5 FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Staining of normal human peripheral blood cells with Anti-Human CD5 FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Immunohistochemistry staining of Paraffin Tonsil tissue by CD5 antibody (dilution: 1:10 - 1:50; visualization of staining: Membranous)
C57BL/6 mouse splenocytes stained with purified 53-7.3, followed by anti-rat IgGs-FITC
Human Tonsil stained with anti-CD5 antibody.
Human B Cell Lymphoma stained with anti-CD5 antibody.
Human Esophagus stained with anti-CD5 antibody.
Human Lung stained with anti-CD5 antibody.
Human Lung Adenocarcinoma stained with anti-CD5 antibody.
Human Prostate stained with anti-CD5 antibody.
Human HK Lymphoma stained with anti-CD5 antibody.
Human Tonsil stained with anti-CD5 antibody.
Human Thymus stained with anti-CD5 antibody.
Human Spleen stained with anti-CD5 antibody.
Human Reactive Lymph Node stained with anti-CD5 antibody.
Human Colon stained with anti-CD5 antibody.
Human Endometrium stained with anti-CD5 antibody.
Human Bladder Transitional Cell Carcinoma stained with anti-CD5 antibody.
Human Tonsil stained with anti-CD5 antibody.
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- Highly Validated (11)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM500009 | CD5 antibody |
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| CD5 mouse monoclonal antibody, clone UMAB9 | |||||||
 |
Mouse | IgG1 | UMAB9 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| UM570009 | CD5 antibody |
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| CD5 mouse monoclonal antibody, clone UMAB9 | |||||||
|
Mouse | IgG1 | UMAB9 | Purified | Hu | F, ICC/IF, WB |
30 µl /
€140.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA353709 | CD5 antibody |
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| Rabbit Monoclonal anti-CD5 Antibody | |||||||
 |
Rabbit | IgG | SP19 | Purified | Hu | P |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody! |
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| TA501335 | CD5 antibody |
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| anti-CD5 mouse monoclonal antibody, clone OTI7A7 (formerly 7A7) | |||||||
 |
Mouse | IgG1 | OTI7A7 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA501376 | CD5 antibody |
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| CD5 mouse monoclonal antibody, clone OTI2B8 (formerly 2B8) | |||||||
 |
Mouse | IgG1 | OTI2B8 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA600467 | CD5 antibody |
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| Luminex and ELISA validated purified CD5 mouse monoclonal antibody clone (2G8, clone OTI2G8 (formerly 2G8) | |||||||
 |
Mouse | IgG1 | OTI2G8 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA600468 | CD5 antibody |
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| Luminex and ELISA validated purified CD5 mouse monoclonal antibody clone (6F7, clone OTI6F7 (formerly 6F7) | |||||||
 |
Mouse | IgG1 | OTI6F7 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody! |
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| TA700464 | CD5 antibody |
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| Luminex and ELISA validated purified biotinylated CD5 mouse monoclonal antibody clone (5D4), clone OTI5D4 (formerly 5D4) | |||||||
 |
Mouse | IgG1 | OTI5D4 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA700465 | CD5 antibody |
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| Luminex and ELISA validated purified biotinylated CD5 mouse monoclonal antibody clone (9E9), clone OTI9E9 (formerly 9E9) | |||||||
 |
Mouse | IgG1 | OTI9E9 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA700466 | CD5 antibody |
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| Luminex and ELISA validated purified biotinylated CD5 mouse monoclonal antibody clone (10H3), clone OTI10H3 (formerly 10H3) | |||||||
 |
Mouse | IgG1 | OTI10H3 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA802427 | CD5 antibody |
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| CD5 mouse monoclonal antibody, clone OTI10F4 (formerly 10F4) | |||||||
 |
Mouse | IgG1 | OTI10F4 | Purified | Hu | F, P |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +17 additional images |
Highly validated antibody!
|
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| DB 027-0.1 | CD5 (C-term) antibody |
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 |
Rabbit | IgG | A25-G | Aff - Purified | P |
0.1 ml /
€310.00
|
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
|
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| DB 027-0.2 | CD5 (C-term) antibody |
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 |
Rabbit | IgG | A25-G | Aff - Purified | P |
0.2 ml /
€420.00
|
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
|
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| DB 027-0.5 | CD5 (C-term) antibody |
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 |
Rabbit | IgG | A25-G | Aff - Purified | P |
0.5 ml /
€540.00
|
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
|
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| DB 027-1 | CD5 (C-term) antibody |
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 |
Rabbit | IgG | A25-G | Aff - Purified | P |
1 ml /
€840.00
|
|
| DB Biotech, spol. s r.o. | |||||||
Acris recommends this product for Immunohistochemistry.
|
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| AM11112PU-N | CD5 antibody |
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 |
Rabbit | SP19 | Supernatant | Hu | P |
1 ml /
€660.00
|
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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| AM11112PU-S | CD5 antibody |
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|
Rabbit | SP19 | Supernatant | Hu | P |
Regular Price: 0.2 ml / €200.00 Special Price: 0.2 ml / €140.00 |
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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| AM50190PU-N | CD5 antibody |
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 |
Mouse | IgG1 | SPM546 | Purified | Hu | F, ICC/IF, P |
0.2 mg /
€410.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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| AM50190PU-S | CD5 antibody |
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|
Mouse | IgG1 | SPM546 | Purified | Hu | F, ICC/IF, P |
0.1 mg /
€320.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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| AM50190PU-T | CD5 antibody |
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|
Mouse | IgG1 | SPM546 | Purified | Hu | F, ICC/IF, P |
20 µg /
€180.00
|
| Acris Antibodies GmbH | |||||||
Acris recommends this product for Immunohistochemistry. |
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