CD50 / ICAM3 antibody

Principal name

CD50 / ICAM3 antibody

Alternative names for CD50 / ICAM3 antibody

ICAM-3, ICAM-R, CDw50, Intercellular adhesion molecule 3

SwissProt ID

P32942 (Human), Q28125 (Bovin)

Gene ID

3385 (ICAM3)

Available reactivities

Hu (Human), Can (Canine), Mky (Monkey), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Functional assay (FN), Neutralisation (NEUT)

Background of CD50 / ICAM3 antibody

ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses. The initial interaction of ICAM-3 with LFA-1 might increase LFA-1-mediated cell binding to ICAM-1. Furthermore, ICAM-3 expression has been shown for dendritic epidermal Langerhans cells, whereas it is absent on other dendritic cells from different lymphoid organs. Thus potential function of ICAM-3 at the initiation phase of LC-leukocyte interactions taking place during skin localized immune reactions can be postulated.

General readings

1. Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernandez-Caselles T, Craig A, Pulido R, Sánchez-Madrid F. ICAM-3 interacts with LFA-1 and regulates the LFA-1/ICAM-1 cell adhesion pathway. J Cell Biol. 1993 Nov;123(4):1007-16.
2. Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM-3, the third LFA-1 counterreceptor, is a co-stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov;23(11):2799-806.
3. Arroyo AG, Campanero MR, Sanchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sanchez-Madrid F: Induction of tyrosine phosphorylation during ICAM-3 and LFA-1-mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep;126(5):1277-86.
4. Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar;17(3):324-33.
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
5. Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol. 2010;2010:841451.
6. Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
7. Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD50 / ICAM3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507134) Mouse IgG1 OTI1E7 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507174) Mouse IgG2b OTI1C2 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG2b SPM505 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG2b SPM505 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG2b SPM505 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI1F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI1F2 Purified Can, Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI1G4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI4F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI4G8 Purified Hu, Mky, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI5D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI4D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI3A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG1 MEM-04 Low Endotoxin Hu F, FN, P
0.1 mg / €210.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

Flow cytometric analysis of CD50 expression on THP-1 cells (left) and HPB-ALL cells (right). Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26548AF-N to the cells. Mouse IgG1 YUK2 Azide Free Hu F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

Flow cytometric analysis of CD50 expression on human lymphocyte (R1), monocyte (R2) and granulocyte (R3) using AM26548RP-N. Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26548RP-N to the cells. Mouse IgG1 YUK2 PE Hu F
50 Tests / €390.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

A typical staining pattern with the B-R1 monoclonal antibody of lymphocytes Mouse IgG1 B-R1 Azide Free Hu F, IP
0.2 mg / €270.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

A typical staining pattern with the B-R1 monoclonal antibody of lymphocytes Mouse IgG1 B-R1 FITC Hu F
1ml (100 Tests) / €270.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

A typical staining pattern with the B-R1 monoclonal antibody of lymphocytes Mouse IgG1 B-R1 Purified Hu F, IP
2ml (200 Tests) / €250.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 2F8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

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