CD50 / ICAM3 antibody

Principal name

CD50 / ICAM3 antibody

Alternative names for CD50 / ICAM3 antibody

ICAM-3, ICAM-R, CDw50, Intercellular adhesion molecule 3

SwissProt ID

P32942 (Human), Q28125 (Bovin)

Gene ID

3385 (ICAM3)

Available reactivities

Hu (Human), Can (Canine), Mky (Monkey), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Frozen Sections (C)

Background of CD50 / ICAM3 antibody

Intercellular adhesion molecules (ICAMs) are type I transmem brane glycoproteins, which contain 2-9 immunoglobulin-like C2-type domains, and bind to lymphocyte function- associated antigen-1 (LFA-1, also known as a L/ ß 2 or CD11a/CD18). ICAM-3 (CD50) is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. ICAM-3 is the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 (CD54) and ICAM-2 (CD102) on resting leukocytes is very low or not at all. Previous studies suggest that ICAM-3 acts as a biologically significant molecule playing a role in HIV-1 transcription and replication, particularly in resting CD4 + T cells. It has been suggested that dendritic cell-specific ICAM grabbing nonintegrin (DC-SIGN, CD209), a C-type lectin of macrophages and DCs, is an additional ligand of ICAM-3. The interaction of DC-SIGN with ICAM-3 establishes initial contact between dendritic cells and resting T-cells during antigen presentation.

General readings

1. Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernandez-Caselles T, Craig A, Pulido R, Sánchez-Madrid F. ICAM-3 interacts with LFA-1 and regulates the LFA-1/ICAM-1 cell adhesion pathway. J Cell Biol. 1993 Nov;123(4):1007-16.
2. Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM-3, the third LFA-1 counterreceptor, is a co-stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov;23(11):2799-806.
3. Arroyo AG, Campanero MR, Sanchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sanchez-Madrid F: Induction of tyrosine phosphorylation during ICAM-3 and LFA-1-mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep;126(5):1277-86.
4. Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar;17(3):324-33.
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
5. Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol. 2010;2010:841451.
6. Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
7. Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI1E7 (formerly 1E7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507134) Mouse IgG1 OTI1E7 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507174) Mouse IgG2b OTI1C2 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG2b SPM505 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG2b SPM505 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG2b SPM505 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

CD50 / ICAM3 antibody

  Rabbit Polyclonal Anti-ICAM3 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Jurkat cells, Primary antibody: (ICAM3 Antibody) at dilution 1/348, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD50 / ICAM3 antibody

  Mouse monoclonal Anti-ICAM3 Clone ICAM 3.1  
CD50 / ICAM3 Mouse IgG1 ICAM 3.1 Purified Hu E, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD50 / ICAM3 antibody

  Mouse monoclonal Anti-ICAM3 Clone ICAM 3.2  
CD50 / ICAM3 Mouse IgG1 ICAM 3.2 Purified Hu E, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD50 / ICAM3 antibody

  Mouse monoclonal Anti-ICAM3 Clone By44  
CD50 / ICAM3 Mouse IgG1 By44 Purified Hu F, IP, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD50 / ICAM3 antibody

  Mouse monoclonal Anti-ICAM3 Clone CG106  
CD50 / ICAM3 Mouse IgG2 CG106 Purified Hu F, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD50 / ICAM3 antibody

  Mouse monoclonal Anti-ICAM3 Clone BU65  
CD50 / ICAM3 Mouse IgG1 Bu65 Purified Hu F, IP
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI1F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI1F2 Purified Can, Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI1G4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI4F5 (formerly 4F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI4F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI4G8 (formerly 4G8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI4G8 Purified Hu, Mky, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI5D6 (formerly 5D6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI5D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI4D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

  ICAM3 mouse monoclonal antibody, clone OTI3A7 (formerly 3A7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3. Mouse IgG2b OTI3A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD50 / ICAM3 antibody

CD50 / ICAM3 Mouse IgG1 MEM-04 Low Endotoxin Hu F, FN, P
0.1 mg / €210.00
  Acris Antibodies GmbH

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