CD54 / ICAM1 antibody

Principal name

CD54 / ICAM1 antibody

Alternative names for CD54 / ICAM1 antibody

ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

SwissProt ID

P05362 (Human), P13597 (Mouse), P33729 (Canfa), Q00238 (Rat), Q95132 (Bovin)

Gene ID

3383 (ICAM1), 25464 (Icam1), 15894, 9332 (CD163)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Ms (Mouse), Can (Canine), Hst (Hamster), Chimp (Chimpanzee), Mky (Monkey), Bab (Baboon), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Hamster, Rabbit, Rat, Sheep, Goat

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), ELISA (capture) (E(capture))

Background of CD54 / ICAM1 antibody

The human ICAM-1 protein consist of 505 amino acids functioning as a type I transmembrane protein belonging to the immunoglobulin superfamily, C2 subset. It contains five Ig-like domains in its extracellular part, a transmembrane domain and a charged cytoplasmic tail. ICAM-1 is differentially glycosylated depending on cell type, giving rise to an 80-114 kDa protein. Deglyosylated ICAM-1 is ~60 kDa. ICAM-1 is expressed by a wide variety of cells including endothelial and epithelial cells, macrophages, fibroblast-like cells and dendritic cells. ICAM-1 expression can be induced by inflammatory cytokines (interleukin-1 (IL-1), Interferon gamma (IFN), tumor necrosis factor alpha (TNFα)), phorbol esters or lipopolysaccharide . Expression regulation is cell specific and depends on the availability of cytokine/hormone receptors, signal transduction pathways, transcription factors, and posttranslational modification.
ICAM-1 is an essential component in many immune-related processes. ICAM-1 links with receptors of
the integrin family, thereby mediating cell-cell interactions and allowing for signal transduction. ICAM-1
interacts specifically with its receptors to induce a reversible adhesion interaction. For processes like T cell activation and leucocyte recruitment, normal immune function relies on ICAM-1. Ligands for ICAM-1 are the Integrins LFA-1 and MAC-1on leukocytes, CD43 (sialophorin), the soluble molecule fibrinogen, the matrix factor hyaluronan, rhinoviruses, and Plasmodium falciparum malaria-infected erythrocytes.
Derangement of ICAM-1 expression contributes to the clinical manifestations of a variety of diseases, predominantly by interfering with normal immune functions. Among these are malignancies, many inflammatory conditions (e.g. asthma, autoimmune disorders), atherosclerosis, ischemia, certain neurological disorders (e.g. multiple sclerosis) and allogeneic organ transplantation.
ICAM-1 is shed by the cell and detected in plasma, CSF and/or BAL as soluble ICAM-1 (sICAM-1). sICAM-1 can be very useful as biomarker for disease activity.

General readings

1. Etienne, S. et al. (1998) ICAM-1 signalling pathways associated with Rho activation
in microvascular brain endothelial cells. J. Immunol. 161: 5755-5761.
2. Etienne, S. et al. (2000) ICAM-1-coupled cytoskeletal rearrangements and
transendothelial lymphocyte migration involve intracellular calcium signaling in brain
endothelial cell lines. J. Immunol. 165: 3375-3383.
3. McKechnie, N. M. et al. (2002) Antigenic mimicry: Onchocerca volvulus antigen-specific
T cells and ocular inflammation. Invest Ophthalmol Vis Sci. 43:411-8.
4. Azcutia, V. et al. (2010) Inflammation determines the pro-adhesive properties of
high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in
vivo. PLoS One 5: e10091
5. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates
after corneal transplantation in rats. Br J Ophthalmol. 2003 Dec;87(12):1515-22.
6. Kielian, T. et al. (2000) Proinflammatory cytokine, chemokine, and cellular adhesion
molecule expression during the acute phase of experimental brain abscess development.
Am J Pathol.157: 647-58.
7. Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis
model: correlation between in vivo confocal microscopy and immunohistochemistry. Mol
Vis. 14:1149-56.
8. Adamson, P. et al. (1999) Lymphocyte migration through brain endothelial cell
monolayers involves signaling through endothelial ICAM-1 via a rho-dependent pathway. J
Immunol. 162: 2964-73.
9. ArsenovićRanin, N. et al. (2000) A monoclonal antibody to the rat Crry/p65 antigen,
a complement regulatory membrane protein, stimulates adhesion and proliferation of
thymocytes. Immunology. 100: 334-44.
10. Beck-Schimmer, B. et al. (2001) Hypoxia mediates increased neutrophil and
macrophage adhesiveness to alveolar epithelial cells. Am J Respir Cell Mol Biol. 25: 780-7.
11. Couty, J.P. (2007) PECAM-1 engagement counteracts ICAM-1-induced signaling in
brain vascular endothelial cells. J Neurochem. 103: 793-801.
12. Deng, H. et al. (2003) Mild hypothermia inhibits inflammation after experimental stroke
and brain inflammation. Stroke. 34: 2495-501.
13. Ikezumi, Y. et al. (2004) Macrophage-mediated renal injury is dependent on signaling
via the JNK pathway. J Am Soc Nephrol. 15: 1775-84.
14. Kanellis, J. et al. (2010) JNK signalling in human and experimental
renal ischaemia/reperfusion injury. Nephrol Dial Transplant. 25: 2898-908.
15. Westermann, D. et al. (2007) Cardioprotective and anti-inflammatory effects of
interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy.
Diabetes. 56: 1834-41.
16. Zhu, X. et al. (2003) Matrine protects sinusoidal endothelial cells from cold ischemia
and reperfusion injury in rat orthotopic liver transplantation. Ann Clin Lab Sci. 33: 216-25.
17. Choi, J.S. et al. (2011) Mild Hypothermia Attenuates Intercellular Adhesion
Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal
Cerebral Ischemia Model. Stroke Res Treat. 2011: 846716.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD54 / ICAM1 antibody

CD54 / ICAM1 Mouse IgG2b D3.6 Purified Hu F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

Peripheral blood lymphocytes stained with PE conjugated CD54 Antibody Cat.-No AM05194RP-N (clone D3.6). Mouse IgG2b D3.6 PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ICAM1 and FGG. HeLa cells were stained with anti-ICAM1 rabbit purified polyclonal 1:1200 and anti-FGG mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD54 / ICAM1 antibody

(2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

Staining of normal human peripheral blood cells with Anti-Human CD54 (ICAM-1) Purified followed by Anti-Mouse IgG FITC . Mouse HA58 Purified Hu F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

Western blot analysis of ICAM-1 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

To detect Human ICAM-1 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

To detect Human ICAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ICAM-1 (TA328339) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human ICAM-1. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

Western blot analysis of extracts of various cell lines, using ICAM1 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

Western blot detection of CD54(ICAM-1) in Raji and SW480 cell lysates and using CD54(ICAM-1) mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 96KDa. Mouse IgG2a 1C7-A8-D6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

Western blot detection of CD54(ICAM-1) in SW480 and Raji cell lysates using CD54(ICAM-1) mouse mAb (1:1000 diluted).Predicted band size:96KDa.Observed band size:96KDa. Mouse IgG2b 4F5-F9-F12 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

TA348959 (0.1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM1 (RC200714, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM1. Mouse IgG1 OTI1E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD54 / ICAM1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ICAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506870) Mouse IgG1 OTI2H4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD54 / ICAM1 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil sections stained with CD54 antibody Cat.-No. AM00428SU-L using peroxidase conjugate and AEC chromogen. Note the cytoplasmic staining of endothelial cells. Mouse IgG1 23G12 Supernatant Hu P
1 ml / €720.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

CD54 / ICAM1 Rat IgG2a BE29G1 Biotin Ms C, F, IP
0.5 mg / €480.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

CD54 / ICAM1 Rat IgG2a BE29G1 Purified Ms C, F, IP
0.5 mg / €380.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

CD54 / ICAM1 Rat IgG2a BE29G1 PE Ms C, F, IP
0.1 mg / €350.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

CD54 / ICAM1 Mouse IgG1 6.5B5 Supernatant Hu C, E, F, IP
2 ml / €460.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

CD54 / ICAM1 Mouse IgG2b 1H4 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

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