CD54 / ICAM1 antibody

Principal name

CD54 / ICAM1 antibody

Alternative names for CD54 / ICAM1 antibody

ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

SwissProt ID

P05362 (Human), P13597 (Mouse), P33729 (Canfa), Q00238 (Rat), Q95132 (Bovin)

Gene ID

3383 (ICAM1), 25464 (Icam1), 15894

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Ms (Mouse), Can (Canine), Mky (Monkey), Sh (Sheep)

Available hosts

Mouse, Hamster, Rabbit, Rat, Sheep, Goat

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), ELISA (capture) (E(capture))

Background of CD54 / ICAM1 antibody

Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein that plays a key role in leukocyte migration and activation . Human ICAM-1 contains five Ig-like domains in its extracellular domain (ECD) and associates into non-covalently linked dimers . Soluble forms of monomeric and dimeric ICAM-1 (sICAM-1) can be generated via proteolytic cleavage by cathepsin G, elastase, MMP-9, MMP-14/MT1-MMP, and TACE/ADAM17 . In the mouse, alternate splicing generates isoforms that lack particular Ig-like domains and are differentially sensitive to proteolysis . Within the ECD, human ICAM-1 shares 53% amino acid sequence identity with mouse and rat ICAM-1.

The principal binding partners of ICAM-1 are the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) . The multivalency of dimeric ICAM-1 increases its strength of interaction with LFA-1 . ICAM-1 also binds several non-integrin ligands including CD43/sialophorin, fibrinogen, hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes . At sites of inflammation, ICAM-1 is upregulated on endothelial and epithelial cells where it mediates the adhesion and paracellular migration of leukocytes expressing activated LFA-1 and Mac-1 . ICAM-1 ligation prolongs antigen presentation by dendritic cells and promotes T cell proliferation and cytokine release . ICAM-1 activation also participates in angiogenesis, wound healing, and bone metabolism .

Soluble ICAM-1 has been reported in serum, cerebrospinal fluid, urine, and bronchoalveolar lavage fluid . Elevated levels of sICAM-1 in these fluids are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies . sICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage . It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis .

General readings

1. Etienne S, Adamson P, Greenwood J, Strosberg AD, Cazaubon S, Couraud PO. ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells. J Immunol. 1998 Nov 15;161(10):5755-61. PubMed PMID: 9820557.
2. Etienne-Manneville S, Manneville JB, Adamson P, Wilbourn B, Greenwood J, Couraud PO. ICAM-1-coupled cytoskeletal rearrangements and transendothelial lymphocyte migration involve intracellular calcium signaling in brain endothelial cell lines. J Immunol. 2000 Sep 15;165(6):3375-83. PubMed PMID: 10975856.
3. McKechnie NM, Gürr W, Yamada H, Copland D, Braun G. Antigenic mimicry: Onchocerca volvulus antigen-specific T cells and ocular inflammation. Invest Ophthalmol Vis Sci. 2002 Feb;43(2):411-8. PubMed PMID: 11818385.
4. Azcutia V, Abu-Taha M, Romacho T, Vázquez-Bella M, Matesanz N, Luscinskas FW, et al. Inflammation determines the pro-adhesive properties of high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in vivo. PLoS One. 2010 Apr 8;5(4):e10091. doi: 10.1371/journal.pone.0010091. PubMed PMID: 20386708.
5. Banerjee S, Figueiredo FC, Easty DL, Dick AD, Nicholls SM. Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats. Br J Ophthalmol. 2003 Dec;87(12):1515-22. PubMed PMID: 14660464.
6. Kielian T, Hickey WF. Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 2000 Aug;157(2):647-58. PubMed PMID: 10934167.
7. Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry. Mol Vis. 2008 Jun 16;14:1149-56. PubMed PMID: 18587491.
8. Adamson P, Etienne S, Couraud PO, Calder V, Greenwood J. Lymphocyte migration through brain endothelial cell monolayers involves signaling through endothelial ICAM-1 via a rho-dependent pathway. J Immunol. 1999 Mar 1;162(5):2964-73. PubMed PMID: 10072547.
9. Arsenović-Ranin N, Vucević D, Okada N, Dimitrijević M, Colić M. A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes. Immunology. 2000 Jul;100(3):334-44. PubMed PMID: 10929055.
10. Beck-Schimmer B, Schimmer RC, Madjdpour C, Bonvini JM, Pasch T, Ward PA. Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells. Am J Respir Cell Mol Biol. 2001 Dec;25(6):780-7. PubMed PMID: 11726405.
11. Couty JP, Rampon C, Leveque M, Laran-Chich MP, Bourdoulous S, Greenwood J, et al. PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells. J Neurochem. 2007 Oct;103(2):793-801. Epub 2007 Jul 27. PubMed PMID: 17662049.
12. Deng H, Han HS, Cheng D, Sun GH, Yenari MA. Mild hypothermia inhibits inflammation after experimental stroke and brain inflammation. Stroke. 2003 Oct;34(10):2495-501. Epub 2003 Sep 11. PubMed PMID: 12970518.
13. Ikezumi Y, Hurst L, Atkins RC, Nikolic-Paterson DJ. Macrophage-mediated renal injury is dependent on signaling via the JNK pathway. J Am Soc Nephrol. 2004 Jul;15(7):1775-84. PubMed PMID: 15213265.
14. Kanellis J, Ma FY, Kandane-Rathnayake R, Dowling JP, Polkinghorne KR, Bennett BL, et al. JNK signalling in human and experimental renal ischaemia/reperfusion injury. Nephrol Dial Transplant. 2010 Sep;25(9):2898-908. doi: 10.1093/ndt/gfq147. Epub 2010 Apr 5. PubMed PMID: 20368303.
15. Westermann D, Van Linthout S, Dhayat S, Dhayat N, Escher F, Bücker-Gärtner C, et al. Cardioprotective and anti-inflammatory effects of interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy. Diabetes. 2007 Jul;56(7):1834-41. Epub 2007 May 1. PubMed PMID: 17473225.
16. Zhu X, Qiu Y, Shi M, Ding Y. Matrine protects sinusoidal endothelial cells from cold ischemia and reperfusion injury in rat orthotopic liver transplantation. Ann Clin Lab Sci. 2003 Spring;33(2):216-25. PubMed PMID: 12817627.
17. Choi JS, Park J, Suk K, Moon C, Park YK, Han HS. Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model. Stroke Res Treat. 2011;2011:846716. doi: 10.4061/2011/846716. Epub 2011 Mar 16. PubMed PMID: 21716663.
18. Azcutia V et al. (2010) Inflammation determines the pro-adhesive properties of high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in vivo. PLoS One. 5 (4): e10091.
19. Li, W. & Klein, S.L. (2012) Seoul virus-infected rat lung endothelial cells and alveolar macrophages differ in their ability to support virus replication and induce regulatory T cell phenotypes. J Virol. 86 (21): 11845-55.
20. Gates, D. et al. (2012) Apo J/clusterin expression and secretion: evidence for 15-deoxy-Δ(12,14)PGJ(2) dependentmechanism. Biochim Biophys Acta. 1821 (2): 335-42.
21. Li, Z. et al. (2015) Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring. Mater Sci Eng C Mater Biol Appl. 57: 181-8.
22. Gautier, S. et al. (2015) PPAR-Alpha Agonist Used at the Acute Phase of Experimental Ischemic Stroke Reduces Occurrence of Thrombolysis-Induced Hemorrhage in Rats. PPAR Research. 2015: 1-6.
23. Liu, Y.C. et al. (2013) A biodegradable, sustained-released, prednisolone acetate microfilm drug delivery system effectively prolongs corneal allograft survival in the rat keratoplastymodel. PLoS One. 8 8): e70419.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD54 / ICAM1 antibody

CD54 / ICAM1 Mouse IgG2b D3.6 Purified Hu F
0.1 mg / €250.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

Peripheral blood lymphocytes stained with PE conjugated CD54 Antibody Cat.-No AM05194RP-N (clone D3.6). Mouse IgG2b D3.6 PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

Western blottin analysis of CD54 expression in activated (A) and non-activated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control. Mouse IgG2a MEM-111 Purified Bov, Hu, Rt E, F, P, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD54 / ICAM1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ICAM1 and FGG. HeLa cells were stained with anti-ICAM1 rabbit purified polyclonal 1:1200 and anti-FGG mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD54 / ICAM1 antibody

  Rabbit anti-ICAM1 (Phospho-Tyr512) polyclonal antibody (Phospho-specific)  
Western blot analysis of extracts from HUVEC cells, using ICAM-1 antibody (Lanes 1, 2 and 3) and ICAM-1 (phospho-Tyr512) antibody (Lanes 4, 5 and 6). Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Goat Anti-ICAM1 Antibody  
(2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Mouse Anti-Human CD54 (ICAM-1) Purified (100 ug)  
Staining of normal human peripheral blood cells with Anti-Human CD54 (ICAM-1) Purified followed by Anti-Mouse IgG FITC . Mouse HA58 Purified Hu F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Rabbit Polyclonal ICAM-1 Antibody  
Western blot analysis of ICAM-1 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Rabbit Polyclonal ICAM-1 (Tyr512) Antibody (Phospho-specific)  
Western blot analysis of ICAM-1 phosphorylation expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Biotinylated Anti-Human ICAM-1 Rabbit Polyclonal Antibody  
To detect Human ICAM-1 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Anti-Human ICAM-1 Rabbit Polyclonal Antibody  
To detect Human ICAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ICAM-1 (TA328339) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human ICAM-1. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Rabbit anti-ICAM1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ICAM1 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Phospho-ICAM1-Y512 Rabbit Polyclonal Antibody  
CD54 / ICAM1 Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Mouse Monoclonal CD54(ICAM-1) Antibody  
Western blot detection of CD54(ICAM-1) in Raji and SW480 cell lysates and using CD54(ICAM-1) mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 96KDa. Mouse IgG2a 1C7-A8-D6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Mouse Monoclonal CD54(ICAM-1) Antibody  
Western blot detection of CD54(ICAM-1) in SW480 and Raji cell lysates using CD54(ICAM-1) mouse mAb (1:1000 diluted).Predicted band size:96KDa.Observed band size:96KDa. Mouse IgG2b 4F5-F9-F12 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Goat Polyclonal Anti-ICAM1 (aa313-327) Antibody  
TA348959 (0.1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Rabbit Polyclonal Anti-ICAM1 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Raji cells, huvec cells, Primary antibody: (ICAM1 Antibody) at dilution 1/1150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Rabbit Polyclonal Anti-ICAM1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Huvec cells, hela cells, raji cells, Primary antibody: (ICAM1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Mouse monoclonal Anti-ICAM1 Clone 15.2  
CD54 / ICAM1 Mouse IgG1 15.2 Purified Hu F, IP, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD54 / ICAM1 antibody

  Mouse monoclonal Anti-ICAM1 Clone P1W16  
CD54 / ICAM1 Mouse IgG1 P1W16 Purified Hu F, ICC/IF, IP, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

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