CD59 antibody

Principal name

CD59 antibody

Alternative names for CD59 antibody

MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, 20 kDa homologous restriction factor

SwissProt ID

O62680 (Pig), O77541 (Rabit), P13987 (Human), P27274 (Rat), P58020 (Sheep)

Gene ID

966 (CD59), 25407 (Cd59)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Ms (Mouse), Mky (Monkey), Bab (Baboon), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit

Available applications

Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), Enzyme Immunoassay (E), Immunoelectron Microscopy (EM), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CD59 antibody

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants (1-8) encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71(1):1-9.
2. Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan
BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83(2):251-6.
3. Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153(6):2444-56.
4. Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173(6):3684-92.
5. Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha). 1986;32(1):12-25. (original description of MEM-43 antigen)
6. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
7. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
8. Robert Sutherland D, Keeney M, Illingworth A: Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry. Cytometry B Clin Cytom. 2012 Apr 12. doi: 10.1002/cyto.b.21023. [Epub ahead of print].
Large number of research studies was published referring the use of the antibody MEM-43, for example:
1. Forsberg UH, Bazil V, Stefanova I, Schroder J.: Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11. Immunogenetics. 1989;30(3):188-93.
2. Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V.: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 Feb;26(2):153-61.
3. Stefanova I, Horejsi V, Ansotegui IJ, Knapp W, Stockinger H.: GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science. 1991 Nov 15;254(5034):1016-9.
4. Cinek T, Horejsi V.: The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol. 1992 Oct 1;149(7):2262-70.
5. Bodian DL, Davis SJ, Morgan BP, Rushmere NK.: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185(3):507-16.
6. Cebecauer M, Cerny J, Horejsi V.: Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun. 1998 Feb 24;243(3):706-10.
7. Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP: Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity. J Immunol. 2006 Mar 1;176(5):2915-23.
8. Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD59 antibody

Western blot analysis of CD59 in mouse spleen tissue lysate with CD59 antibody at 1 ug/mL Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD59 antibody

Western blot analysis of extracts of BXPC-3 cell lines, using CD59 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD59 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD59 (RC218343, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD59. Mouse IgG1 OTI2E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD59 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD59 (RC200736, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD59. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD59 antibody

Cell Source: Lymphocytes Mouse IgG1 1F5 Biotin Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD59 antibody

Cell Source: Lymphocytes Mouse IgG1 1F5 FITC Hu F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD59 antibody

Placenta: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG1 1F5 Purified Hu F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD59 antibody

Cell Source: Lymphocytes Mouse IgG1 1F5 PE Hu F
50 µg / €260.00
  Acris Antibodies GmbH

CD59 antibody

Skin, pilosebaceous unit: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG2b MEM-43/5 Purified Hu, Ms F, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD59 antibody

AM08350PU-N at 10 µg/ml staining CD59 by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-Embedded Placenta Sections). Mouse IgG2a p282 (H19) Purified Bab, Hu, Mky, Prim F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD59 antibody

Flow cytometric analysis of CD59 expression on HPB-ALL. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26551AF-N to the cells. Mouse IgG2b WK8 Azide Free Hu F, IP
0.1 mg / €340.00
  Acris Antibodies GmbH

CD59 antibody

Flow cytometric analysis of CD59 expression on human lymphocyte (R1), monocyte (R2) and granulocyte (R3) using AM26551FC-S. Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26551FC-S to the cells. Mouse IgG2b WK8 FITC Hu F
0.1 ml / €390.00
  Acris Antibodies GmbH

CD59 antibody

CD59 Mouse IgG1 TH9 Purified Rt C, F, FN, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD59 antibody

Representative Data
Staining with clone NaM172-2B5 (anti-CD59) monoclonal antibody is illustrated by flow cytometry analysis of normal blood cells. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl red blood cell suspension. Mouse IgG1 NaM172-2B5 FITC Hu C, F, ICC/IF
100 Tests / €350.00
  Acris Antibodies GmbH

CD59 antibody

Representative Data
Staining with clone NaM172-2B5 (anti-CD59) monoclonal antibody is illustrated by flow cytometry analysis of normal blood cells. Direct staining was performed using 10 µl of the FITC-conjugated antibody and 100 µl red blood cell suspension. Mouse IgG1 NaM172-2B5 PE Hu C, F, ICC/IF
100 Tests / €400.00
  Acris Antibodies GmbH

CD59 antibody

CD59 Mouse IgG1 1B4 Purified Rt F, IP, WB
0.25 mg / €440.00
  Acris Antibodies GmbH

CD59 antibody

CD59 Mouse IgG1 1B4 Purified Rt F, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

CD59 antibody

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD59: AZIDE FREE (SM1145A). Mouse IgG2a MEM-43 Azide Free Hu C, E, F, IP, P, WB
1 mg / €920.00
  Acris Antibodies GmbH

CD59 antibody

CD59 Mouse IgG2a MEM-43 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD59 antibody

Staining of KG1  lymphocytes with Mouse Anti Human CD59-FITC (SM1145FT). Mouse IgG2a MEM-43 FITC Hu F
25 µg / €190.00
  Acris Antibodies GmbH

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