CD59 antibody

Principal name

CD59 antibody

Alternative names for CD59 antibody

MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, 20 kDa homologous restriction factor

SwissProt ID

O62680 (Pig), O77541 (Rabit), P13987 (Human), P27274 (Rat), P58020 (Sheep)

Gene ID

966 (CD59), 25407 (Cd59)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Ms (Mouse), Mky (Monkey), Prim (Primate), Bab (Baboon)

Available hosts

Mouse, Rat, Rabbit

Available applications

Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), Enzyme Immunoassay (E), Immunoelectron Microscopy (EM), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CD59 antibody

Rat CD59 was previously known as rat inhibitory protein (RIP) and it is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization. CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes. In rat, CD59 is expressed on vacular endothelium and circulating cells. In the central nervous system (CNS) of the rat, CD59 is expressed on the Schwann sheath of peripheral nerve fibres and on ependymal cells, but not on glial cells and neurons in the CNS. Rat astrocytes in vitro express CD59 on its surface.
CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells.

General readings

1. Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71(1):1-9.
2. Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan
BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83(2):251-6.
3. Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153(6):2444-56.
4. Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173(6):3684-92.
5. Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha). 1986;32(1):12-25. (original description of MEM-43 antigen)
6. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
7. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
8. Robert Sutherland D, Keeney M, Illingworth A: Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry. Cytometry B Clin Cytom. 2012 Apr 12. doi: 10.1002/cyto.b.21023. [Epub ahead of print].
Large number of research studies was published referring the use of the antibody MEM-43, for example:
1. Forsberg UH, Bazil V, Stefanova I, Schroder J.: Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11. Immunogenetics. 1989;30(3):188-93.
2. Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V.: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 Feb;26(2):153-61.
3. Stefanova I, Horejsi V, Ansotegui IJ, Knapp W, Stockinger H.: GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science. 1991 Nov 15;254(5034):1016-9.
4. Cinek T, Horejsi V.: The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol. 1992 Oct 1;149(7):2262-70.
5. Bodian DL, Davis SJ, Morgan BP, Rushmere NK.: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185(3):507-16.
6. Cebecauer M, Cerny J, Horejsi V.: Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun. 1998 Feb 24;243(3):706-10.
7. Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP: Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity. J Immunol. 2006 Mar 1;176(5):2915-23.
8. Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8.

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Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD59 (transcript variant 2)

  Recombinant protein of human CD59 molecule, complement regulatory protein (CD59), transcript variant 2  
CD59 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  OriGene Technologies GmbH

CD59 (26-102, His-tag)

CD59 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  OriGene Technologies GmbH

3 Item(s)

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