CD62E / E-Selectin antibody

Principal name

CD62E / E-Selectin antibody

Alternative names for CD62E / E-Selectin antibody

SELE, ELAM1, LECAM2, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, CD62 antigen-like family member E

SwissProt ID

P16581 (Human), P27113 (Rabit), P33730 (Canfa), P98105 (Rat), P98107 (Bovin), P98110 (Pig), Q00690 (Mouse), Q95LG1 (Horse)

Gene ID

6401 (SELE), 20339 (Sele), 25544

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Mky (Monkey), Chimp (Chimpanzee), Bab (Baboon)

Available hosts

Rat, Mouse, Rabbit, Sheep

Available applications

Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), ELISA (capture) (E(capture)), Luminex (LMNX)

Background of CD62E / E-Selectin antibody

E-Selectin (also known as Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E) is a 115 kDa, type I transmembrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines (IL-1. or TNF-.) or endotoxins . Expression is transitory, reaching a maximum within about 6 hours of stimulation and then declining with shedding of soluble E-Selectin . Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in extravasation of leukocytes at the site of inflammation thereby playing a key role in localized inflammatory response. E-Selectin is believed to be particularly important in inflammation involving the skin .

The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatory-protein-like sequence . E-Selectin binds sialyl Lewis X (sLex), a sialic acid-galactose-N-acetylglucosamine-fructose tetrasaccharide, but the actual recognition is thought to be for a specific presentation of those glycosyl units in a precise three-dimensional configuration on a specific glycoprotein rather than for that particular carbohydrate .

Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals , probably arising from proteolytic cleavage of the surface-expressed molecule . Elevated levels of sE-Selectin in serum have been reported in a variety of pathological conditions . Although it might be anticipated that sE-Selectin would suppress leukocyte migration by competing with surface-associated E-Selectin, it may actually activate neutrophils and act as a pro-inflammatory agent .

General readings

Bevilacqua, M.P. and R.M. Nelson (1993) J. Clin. Invest. 91:379.

McEver, R.P. (1994) Curr. Opin. Immunol. 6:75.

Tedder, T.F. et al. (1995) FASEB J. 9:866.

Kansas, G.S. (1996) Blood 88:3259.

Wyble, C.W. et al. (1997) J. Surg. Res. 73:107.

Kunkel, E.J. and K. Ley (1996) Circ. Res. 79:1196.

Lobb, R.R. et al. (1991) J. Immunol. 147:124.

Gearing, A.J.H. et al. (1992) Annals N.Y. Acad. Sci. 667:324.

Morandini, R. et al. (1996) Am. J. Physiol. 270:807.

Hebbar, M. et al. (1998) Clin. Cancer Res. 4:373.

Ohsaka, A. et al. (1998) Br. J. Haematol. 100:66.

Frijns, C.J.M. et al. (1997) Stroke 28:2214.

Okajima, K. et al. (1997) Am. J. Hematol. 54:219.

Matsuura, N. et al. (1997) Anticancer Res. 17:1367.

Ruchaud-Sparagano, M-H. et al. (1998) Eur. J. Immunol. 28:80.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD62E / E-Selectin antibody

Western blot analysis of E-Selectin with Jurkat cell lysate  35 Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD62E (E- Selectin) Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Total viable cells were used for analysis. Mouse P2H3 Purified Hu F, IP, P
0.1 mg / €130.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian and gastric cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SELE antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

Predicted cell location: Cell membrane,Cytoplasm . Positive control: Human esophagus and lung cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using SELE antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

Western blot analysis of extracts of various cell lines, using SELE antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

SELE ELISA with CL-37 Capture (TA600028) and CL2 Detection (TA700028) Antibodies. Substrate used: Recombinant Human SELE (TP321153) Mouse IgG1 OTICL-37 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

SELE ELISA with CL-37 Capture (TA600028) and CL2 Detection (TA700028) Antibodies. Substrate used: Recombinant Human SELE (TP321153) Mouse IgG1 OTICL2 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD62E / E-Selectin antibody

CD62E / E-Selectin Rat IgG2a 4D10 FITC Hu F
0.5 mg / €420.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Mouse IgG1 ENA2 Purified Hu, Mky C, E, FN
0.1 mg / €350.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Mouse IgG1 ENA1 Purified Hu, Mky C, E, FN, IP
0.1 mg / €350.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

A typical staining pattern with the B-P7 monoclonal antibody of ELAM-1 transfected CHO cell line Mouse IgG1 B-P7 Azide Free Hu F
0.2 mg / €270.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

A typical staining pattern with the B-P7 monoclonal antibody of ELAM-1 transfected CHO cell line Mouse IgG1 B-P7 Purified Hu F
2ml (200 Tests) / €250.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Mouse IgG1 B-P7 Azide Free Hu E(capture)
1 mg / €790.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Mouse IgG1 B-S3 Biotin Hu E(detection)
0.1 mg / €460.00
  Acris Antibodies GmbH

CD62E / E-Selectin F(ab')2 antibody

CD62E / E-Selectin Mouse IgG1 ENA2 Liquid Bab, Chimp, Hu, Mky C, E, FN
1 ml / €650.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Mouse IgG1 ENA1 Biotin Hu C
0.5 ml / €390.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Mouse IgG1 ENA1 Liquid Hu C
1 ml / €390.00
  Acris Antibodies GmbH

CD62E / E-Selectin control peptide

CD62E / E-Selectin Purified
50 µg / €190.00
  Acris Antibodies GmbH

CD62E / E-Selectin antibody

CD62E / E-Selectin Rabbit IgG Biotin Hu, Mky C, E, WB
50 µg / €460.00
  Acris Antibodies GmbH

CD62E / E-Selectin (+CD62P) antibody

Staining of Thrombin activated Human peripheral blood platelets with Mouse anti Human CD62E/CD62P antibody Mouse IgG1 1.2B6 Purified Hu, Por C, E, F, ICC/IF, WB
0.2 mg / €480.00
  Acris Antibodies GmbH

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