CD62L / L-Selectin antibody

Principal name

CD62L / L-Selectin antibody

Alternative names for CD62L / L-Selectin antibody

SELL, LNHR, LYAM1, Leu-8, TQ1, gp90-MEL, LECAM1, LAM-1, Lymph node homing receptor, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, CD62 antigen-like family member L

SwissProt ID

P14151 (Human), P18337 (Mouse), P30836 (Rat), P98131 (Bovin)

Gene ID

6402 (SELL), 20343 (Sell), 281485, 6402186 (accA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey)

Available hosts

Rabbit, Rat, Mouse, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD62L / L-Selectin antibody

L-Selectin (CD62L) is a type I membrane protein expressed on the surface of T cells, B cells, monocytes, neutrophils, macrophages, eosinophils and NK cells. L-Selectin mediates entry of lymphocytes into secondary lymphoid tissue by adherence to endothelial cells of high endothelial venules (HEV). Recruitment of leukocytes to sites of inflammation also involves L-Selectin dependent adhesion to the endothelium. L-Selectin has been considered as a suitable target for treatment or prevention of inflammatory diseases such as asthma, rheumatoid arthritis, inflammatory bowel disease, allergies and atherosclerosis. L-Selectin is a glycoprotein composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two Sushi consensus domains, a transmembrane domain and a short cytoplasmic tail. L-Selectin is extensively glycosylated and different glycoforms exist on different leukocyte subsets. Moreover, different L-Selectin glycosylation patterns influence the binding activity to various ligands on the endothelium. L-Selectin contains 7 potential N-linked glycosylation sites. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

1. Fink, P., W. Gallatin, R. Reichert, et al. 1985. Homing receptor-bearing thymocytes, an immunocomponent cortical subpopulation. Nature 313: 233-235
2. Gallatin,W.M., I.L. Weissman., E.C. Butcher 1983. A cell surface molecule involved in organ specific homing of lymphocytes. Nature 304:30-34
3. Lewinsohn, D.M., R.F. Bargatze, E.C. Butcher 1987. Leukocyteendothelial cell recognition: evidence of a common molecular mechanism
shared by neutrophils, lymphocytes and other leukocytes. J.Immunology 138:4313-4321
4. Reichert, R., M. Gallitin, E. Butcher, et al.. 1984. A homing receptorbearing cortical thymocyte subset: Implications for thymus cell migration and the nature of cortisone-resisatant thymocytes. Cell 38: 89-99
5. Siegelman, M., I.C. Cheng, I.L. Weissman, et al. 1990. The mouse lymph node homing receptor is identical with the lymphocyte cell surface receptor Ly-22: Role of the EGF domain in endothelial binding. Cell 61: 611-622
6. Jalkanen, S., R.F. Bargatze, J. Toyos, et al. 1987. Lymphocyte Recognition of High Endothelium: Antibodies to Distinct Epitopes of an 85-95-kD Glycoprotein Antigen Differentially Inhibit Lymphocyte Binding to Lymph Node, Mucosal, or Synovial Endothelial Cell. J. of Cell Biol. 105: 983-990

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD62L / L-Selectin antibody

Western blot analysis of L-Selectin expression in Jurkat cell lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62L / L-Selectin antibody

Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD62P (P-Selectin) Purified (filled histogram) followed by  Anti-Mouse IgG FITC .  Cells in the lymphocyte gate were used for analysis. Mouse DREG-56 (DREG56) Purified Hu F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD62L / L-Selectin antibody

Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purifed (open histogram) or 0.25 ug of Anti-Mouse CD62L (L-Selectin) Purified (filled histogram) followed by Anti-Rat IgG FITC .  Cells in the lymphocyte gate were used for analysis. Rat MEL-14 Purified Ms F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD62L / L-Selectin (C-term) antibody

Western blot analysis of SELL Antibody (C-term) (Cat. #TA324887) in Jurkat cell line lysates (35ug/lane).SELL (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD62L / L-Selectin antibody

Western blot analysis of extracts of various cell lines, using SELL antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62L / L-Selectin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SELL (RC216982, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SELL. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62L / L-Selectin antibody

Surface staining of human peripheral blood cells with anti-CD62L (LT-TD180) purified, GAM-APC. Mouse IgG1 LT-TD180 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

Surface staining of human peripheral blood cells with anti-CD62L (LT-TD180) APC. Mouse IgG1 LT-TD180 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

Surface staining of human peripheral blood cells with anti-CD62L (LT-TD180) APC. Mouse IgG1 LT-TD180 Purified Hu F
0.1 mg / €210.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

Surface staining of human peripheral blood cells with anti-CD62L (LT-TD180) APC. Mouse IgG1 LT-TD180 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

CD62L / L-Selectin Mouse IgG2a 1H3 FITC Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

CD62L / L-Selectin Mouse IgG2a 1H3 Purified Hu F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

CD62L / L-Selectin Mouse IgG2a 1H3 PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

Surface staining of CD62L in human peripheral blood with anti-CD62L (DREG56) Alexa Fluor 700. Mouse IgG1 DREG56 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

Surface staining of CD62L in human peripheral blood with anti-CD62L (DREG56) Alexa Fluor 700. Mouse IgG1 DREG56 Low Endotoxin Hu C, F, FN, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

Surface staining of CD62L in human peripheral blood with anti-CD62L (DREG56) Alexa Fluor 700. Mouse IgG1 DREG56 Purified Hu C, F, FN, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

A typical staining pattern with the B-S13 monoclonal antibody of lymphocytes Mouse IgG1 B-S13 Azide Free Hu E, F
0.2 mg / €270.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

A typical staining pattern with the B-S13 monoclonal antibody of lymphocytes Mouse IgG1 B-S13 Purified Hu F
2ml (200 Tests) / €250.00
  Acris Antibodies GmbH

CD62L / L-Selectin antibody

A typical staining pattern with the B-S13 monoclonal antibody of lymphocytes Mouse IgG1 B-S13 PE Hu F
1ml (100 Tests) / €290.00
  Acris Antibodies GmbH

CD62L / L-Selectin Control Peptide

CD62L / L-Selectin Purified
50 µg / €190.00
  Acris Antibodies GmbH

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