CD62P / P-Selectin antibody

Principal name

CD62P / P-Selectin antibody

Alternative names for CD62P / P-Selectin antibody

SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, CD62 antigen-like family member P

SwissProt ID

P16109 (Human), P42201 (Bovin), P98106 (Rat), P98109 (Sheep), Q01102 (Mouse)

Gene ID

6403 (SELP), 20344 (Selp), 25651

Available reactivities

Hu (Human), Mky (Monkey), Bov (Bovine), Eq (Equine), Fe (Feline), Gt (Goat), Ms (Mouse), Rt (Rat), Sh (Sheep), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Enzyme Immunoassay (E)

Background of CD62P / P-Selectin antibody

P-Selectin (GMP-140, LECAM-3, PADGEM, CD62, CD62P) is a cell surface glycoprotein that plays a critical role in the migration of lymphocytes into tissues . It is found constitutively in a pre-formed state in the Weibel-Palade bodies of endothelial cells and in the alpha granules of platelets . This stored P-Selectin is mobilized to the cell surface within minutes in response to a variety of inflammatory or thrombogenic agents . The mobilized P-Selectin is apparently present on the cell surface for only a few minutes after which it is recycled to intracellular compartments . Additional evidence indicates that transcription of P-Selectin mRNA can be activated in the endothelium by treatment with inflammatory mediators .P-Selectin consists of an NH2-terminal lectin type C domain, an EGF-like domain, nine complement control domains, a transmembrane domain, and a short cytoplasmic domain . Mouse P-Selectin shows a similar organization of functional domains and an overall sequence identity of approximately 73% . However, mouse P-Selectin contains only eightcomplement control domains, suggesting that the absolute number of these domains is not crucial for function . The molecular weight predicted from the cDNA for P-Selectin is approximately 86,000 . The observed molecular weight on reducing SDS-PAGE,however, is approximately 140,000 .

Evidence indicates that P-Selectin is involved in the adhesion of myeloid cells, as well as B cells and a subset of T cells, to activated endothelium . P-Selectin is also involved in the adhesion of platelets to monocytes and neutrophils, playing a central role in neutrophil accumulation within thrombi . The adhesion of leukocytes and neutrophils to the endothelium is initiated by weak interactions that produce a characteristic Â?rollingÂ? motion of the leukocytes and neutrophils on the endothelial surface . P-Selectin, acting in cooperation with L-Selectin, is implicated in the mediation of these initial interactions . Stronger interactions, probably involving E-Selectin, follow the initial interactions, leading eventually to extravasation through the blood vessel walls into lymphoid tissues and to sites of inflammation . The tetrasaccharide sialyl Lewisx (sLex) has been identified as a ligand for both P- and E-Selectin, but P-, E- and L-Selectin can all bind sLex and sLea under appropriate conditions . P-Selectin also reportedly binds selectively to a 160 kDa glycoprotein present on mouse myeloid cells, and to a glycoprotein on human myeloid cells, blood neutrophils, monocytes, and lymphocytes termed P-Selectin glycoprotein ligand-1 (PSGL-1), a ligand that also can bind E-Selectin . P-Selectin-mediated rolling of leukocytes can be completely inhibited by a monoclonal antibody specific for PSGL-1, suggesting that even though P-Selectin can bind to a variety of glycoproteins under in vitro conditions, it is likely that physiologically important binding is more limited .

P-Selectin is found in the plasma of normal individuals at ng/mL concentrations . Circulating P-Selectin appears to be slightly smaller than native P-Selectin. An alternatively spliced mRNA encoding a form of human P-Selectin lacking the transmembrane anchoring domain has been reported for both megakaryocytes and endothelial cells , and evidenc suggests that the majority of circulating soluble P-Selectin arises in this manner . A number of studies have reported that levels of soluble P-Selectin in biological fluids may be elevated in subjects with a variety of pathological conditions .

General readings

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, pp. 132.

Barclay, A.N. et al. (1993) in The Leukocyte Antigen Facts Book, pp. 240.

Ley, K. and T.F. Tedder (1995) J. Immunol. 155:525.

Tedder, T.F. et al. (1995) FASEB J. 9:866.

Lasky, L. (1995) Annu. Rev. Biochem. 64:113.

Weller, A. et al. (1992) J. Biol. Chem. 267:15176.

Lasky, L.A. (1993) Current Biol. 3:680.

Sako, D. et al. (1993) Cell 75:1179.

Moore, K.L. et al. (1995) J. Cell Biol. 128:661.

Gearing, A.J.H. and W. Newman (1993) Immunol. Today 14:506.

Ishiwata, N. et al. (1994) J. Biol. Chem. 265:21381.

Takeda, I. et al. (1994) Allergy Immunol. 105:128.

Katayama, M. et al. (1993) Br. J. Haematol. 84:702.

Facer, C.A. and A. Theodoridou (1994) Microbiol. Immunol. 38:727.

Gruschwitz, M.S. et al. (1995) Arthritis Rheum. 38:184.

Ikeda, H. et al. (1994) Coron. Artery Dis. 5:515.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD62P / P-Selectin antibody

Staining of thrombin activated human peripheral blood platelets with Mouse Anti-Human CD62P Antibody FITC conjugated (SM1150F/FT). Mouse IgG1 AK-6 FITC Hu, Mky F
0.1 mg / €410.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

Staining of thrombin activated human peripheral blood platelets with Mouse Anti-Human CD62P Antibody FITC conjugated (SM1150F/FT). Mouse IgG1 AK-6 FITC Hu, Mky F
25 µg / €190.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

  Rabbit polyclonal anti-P-Selectin antibody  
Western blot analysis of P- Selectin expression in Jurkat cell lysate. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62P / P-Selectin antibody

  Mouse Anti-Human CD62P (P-Selectin) Purified (100 ug)  
Staining of thrombin-activated human peripheral blood platelets with 0.5 ug of Mouse IgG1 kappa Isotype Control Purifed (open histogram) or 0.5 ug of Anti-Human CD62P (P-Selectin) Purified (filled histogram) followed by Anti-Mouse IgG FITC .  Total viable cells were used for analysis. Mouse AK-4 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD62P / P-Selectin antibody

  Mouse Monoclonal anti-CD62P (P-Selectin) Antibody  
Thrombin-activated human peripheral blood platelets stained with purified AK4, followed by anti-mouse IgGs FITC Mouse AK4 Purified Hu F, P
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD62P / P-Selectin antibody

  Rabbit Polyclonal CD62P Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SELP (RC209822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SELP. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 HI62P Aff - Purified Hu F, WB
0.1 mg / €220.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

Immunohistochemistry: AM05567PU-N SELP antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 Psel.KO.2.5 Purified Hu, Por, Sh F, IP, P
0.2 mg / €430.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

Staining of thrombin activated peripheral blood platelets with mouse anti human CD62P Antibody -RPE (Cat.-No AM05567RP-N) Mouse IgG1 Psel.KO.2.5 PE Hu, Por F
100 Tests / €470.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 AK4 APC Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 AK4 Biotin Hu, Prim F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 AK4 FITC Hu, Prim F
100 Tests / €260.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 AK4 Low Endotoxin Hu, Prim
0.1 mg / €210.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 AK4 Aff - Purified Hu, Prim F, FN, ICC/IF, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 AK4 PE Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG2b B-G43 Azide Free Hu
0.2 mg / €270.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

A typical staining pattern with the B-F46 monoclonal antibody of platelets. Mouse IgG1 B-F46 Azide Free Hu
0.2 mg / €270.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

A typical staining pattern with the B-F46 monoclonal antibody of platelets. Mouse IgG1 B-F46 Purified Hu F
2ml (200T) / €250.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 CRC81 Purified Hu ICC/IF, WB
1 ml / €350.00
  Acris Antibodies GmbH

CD62P / P-Selectin antibody

CD62P / P-Selectin Mouse IgG1 CRC81 PE Hu
0.5 ml / €470.00
  Acris Antibodies GmbH

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