CD63 antibody

Principal name

CD63 antibody

Alternative names for CD63 antibody

OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491, Melanoma-associated antigen ME491, Ocular melanoma-associated antigen

SwissProt ID

P08962 (Human), P28648 (Rat), P41731 (Mouse), Q28709 (Rabit), Q76B49 (Felca), Q9XSK2 (Bovin)

Gene ID

967 (CD63), 12512 (Cd63)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Mky (Monkey), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Goat

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Functional assay (FN)

Background of CD63 antibody

CD63 is not only expressed on activated platelets, but also activated monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and translocated to cell surface upon various stimuli. The membrane of lytic granules in CTLs contains CD63/LAMP-3 and other lysosomal-associated glycoproteins (LAMPs) such as CD107a/LAMP-1 and CD107b/LAMP-2. LAMPs have b een observed on the cell surface as a result of degranulation. CD63 belongs to a member of the tetraspanin transmembrane-protein (TM4) superfamily, which includes CD9, CD37, CD53, CD81, CD82, CD151 and CD231. Several members of this family form noncovalent associations with integrins, particularly ß 1 integrins (CD29), and modulate cellular adhesion properties. CD63 has a tyrosine-based internalization motif in the cytoplasmic C-terminal tail and interacts with adaptor protein complexes such as AP-2 and AP-3. Because AP-2 and AP-3 are involved in facilitating the clathrin-mediated endocytosis, CD63 could be directly involved in the internalization of its membrane protein partners.

General readings

Modderman, P.W., in Leukocyte Typing IV (W. Knapp et al., eds.), p. 1042

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD63 antibody

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 antibody (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG1 MEM-259 Purified Hu F, ICC/IF, IP, P, WB
0.1 mg / €220.00
  Acris Antibodies GmbH

CD63 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802751) Mouse IgG2a OTI2G6 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD63 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802811) Mouse IgG1 OTI1E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD63 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD63 mouse monoclonal antibody. (TA803392; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG2a OTI1D12 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD63 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD63 mouse monoclonal antibody. (TA803394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI2A2E2 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD63 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803574) Mouse IgG1 OTI5E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD63 antibody

Fig. 3. Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG1 MEM-259 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD63 antibody

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue). Mouse IgG1 MEM-259 Biotin Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD63 antibody

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG1 MEM-259 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD63 antibody

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue). Mouse IgG1 MEM-259 PerCP Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD63 antibody

Fig. 3. Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG1 MEM-259 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD63 (Late Endosomes Marker) antibody

Formalin-paraffin human melanoma stained with CD63 MAb (Clone SPM524) Mouse IgG1 SPM524 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD63 (Late Endosomes Marker) antibody

Formalin-paraffin human melanoma stained with CD63 MAb (Clone SPM524) Mouse IgG1 SPM524 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD63 (Late Endosomes Marker) antibody

Formalin-paraffin human melanoma stained with CD63 MAb (Clone SPM524) Mouse IgG1 SPM524 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD63 (Late Endosomes Marker) antibody

Formalin-paraffin human melanoma stained with CD63 MAb (Clone NKI/C3) Mouse IgG1 NKI/C3 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD63 (Late Endosomes Marker) antibody

Formalin-paraffin human melanoma stained with CD63 MAb (Clone NKI/C3) Mouse IgG1 NKI/C3 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD63 (Late Endosomes Marker) antibody

Formalin-paraffin human melanoma stained with CD63 MAb (Clone NKI/C3) Mouse IgG1 NKI/C3 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD63 antibody

Immunohistochemistry staining of Paraffin Melanoma tissue by CD63 antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic, membranous) Mouse IgG1 NKI/C3 Purified Hu P
1 ml / €340.00
  OriGene Technologies, Inc.

CD63 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (RC201733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63. Mouse IgG1 OTI1E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD63 antibody

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802752) Mouse IgG1 OTI1E3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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