CD66a / CEACAM1 antibody

Principal name

CD66a / CEACAM1 antibody

Alternative names for CD66a / CEACAM1 antibody

BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1

SwissProt ID

P13688 (Human), P16573 (Rat), P31809 (Mouse)

Gene ID

634 (CEACAM1), 26365 (Ceacam1)

Available reactivities

Hu (Human), Mam (Mammalia), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunoprecipitation (IP)

Background of CD66a / CEACAM1 antibody

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa's 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD66a / CEACAM1 antibody

Immunohistochemical staining on cryosections of colon tissue using 8G5 (DM1201). PBS containing 2.5% horse serum served as negative control (A). Detection of 8G5 occurred with a bioti-nylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminoben-zidine was used as substrate. Nuclei were stained with hematoxilin. Mouse IgG1 8G5 Purified Hu, Mam C, E, F, ICC/IF
0.1 mg / €360.00
  Acris Antibodies GmbH

CD66a / CEACAM1 antibody

  Rabbit Polyclonal CEACAM1 Antibody  
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CEACAM1 Rabbit Polyclonal antibody. (TA590096) Rabbit IgG Purified Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD66a / CEACAM1 antibody

Western blot: Human CEACAM-1 lysate Detection utilysing 10 μg/ml. Mouse IgG1 C5-1X Purified Hu E, F, ICC/IF, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD66a / CEACAM1 antibody

IHC staining of human jejunum tissue with Monoclonal Antibody B3. CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using B3 followed by staining with anti-mouse HRP-DAB and counterstaining with hematoxylin. The labeling showed week staining of CEACAM1 by B3. Mouse IgG1 B3 Purified Hu E, F, ICC/IF, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD66a / CEACAM1 antibody

  Mouse Anti-Mouse CD66a (CEACAM1) Purified (25 ug)  
CD66a / CEACAM1 Mouse CC1 Purified Ms F, P
25 µg / €120.00
  OriGene Technologies, Inc.

CD66a / CEACAM1 antibody

  CEACAM1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CEACAM1 antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD66a / CEACAM1 antibody

  Rabbit Polyclonal Anti-CEACAM1 Antibody (Internal)  
Anti-CEACAM1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

CD66a / CEACAM1 antibody

  Rabbit Polyclonal Anti-CEACAM1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Human metastatic malignant melanoma tissue, Primary antibody: (CEACAM1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD66a / CEACAM1 antibody

A typical staining pattern with the B-D60 FITC monoclonal antibody of granulocytes Mouse IgG1 B-D60 FITC Hu F
1ml (100T) / €270.00
  Acris Antibodies GmbH

CD66a / CEACAM1 antibody

Flow cytometric analysis with the B-D60 monoclonal antibody on granulocytes Mouse IgG1 B-D60 Purified Hu F
2ml (200T) / €250.00
  Acris Antibodies GmbH

CD66a / CEACAM1 antibody

A typical staining pattern with the B-D60 PE monoclonal antibody of granulocytes Mouse IgG1 B-D60 PE Hu F
1ml (100T) / €290.00
  Acris Antibodies GmbH

CD66a / CEACAM1 antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2F6 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CD66a / CEACAM1 (D1-4) antibody

Western Analysis with anti-Human CEACAM1. Both antibodies recognize CEACAM1 but not CEACAM8. Cross reaction with CEACAM3, CEACAM4, CEACAM5, CEACAM6 und CEACAM7 are not tested so far. The experiment was performed by Dr. Bernhard Singer, University Hospital Essen, Germany Rabbit Aff - Purified Hu E, F, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

CD66a / CEACAM1 (D1-4) antibody

Western Analysis with anti-Human CEACAM1. Both antibodies recognize CEACAM1 but not CEACAM8. Cross reaction with CEACAM3, CEACAM4, CEACAM5, CEACAM6 und CEACAM7 are not tested so far. The experiment was performed by Dr. Bernhard Singer, University Hospital Essen, Germany Rabbit Aff - Purified Hu E, F, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD66a / CEACAM1 (N-term) antibody

Western Analysis with anti-Human CEACAM1. Both antibodies recognize CEACAM1 but not CEACAM8. Cross reaction with CEACAM3, CEACAM4, CEACAM5, CEACAM6 und CEACAM7 are not tested so far.The experiment was performed by Dr. Bernhard Singer, University Hospital Essen, Germany Rabbit Aff - Purified Hu E, F, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

CD66a / CEACAM1 (N-term) antibody

Western Analysis with anti-Human CEACAM1. Both antibodies recognize CEACAM1 but not CEACAM8. Cross reaction with CEACAM3, CEACAM4, CEACAM5, CEACAM6 und CEACAM7 are not tested so far.The experiment was performed by Dr. Bernhard Singer, University Hospital Essen, Germany Rabbit Aff - Purified Hu E, F, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD66a / CEACAM1 (Extracell. Dom.) antibody

CD66a / CEACAM1 Mouse IgG1 283324 Aff - Purified Hu E, E(capture), WB
0.5 mg / €410.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

CEACAM1 - human gene knockout kit via CRISPR

CEACAM1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CEACAM1 - human gene knockout kit via CRISPR

CEACAM1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CEACAM1 - human gene knockout kit via CRISPR

CEACAM1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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