CD69 antibody

Principal name

CD69 antibody

Alternative names for CD69 antibody

GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM, Early T-cell activation antigen p60, Early activation antigen CD69, Activation inducer molecule, C-type lectin domain family 2 member C

SwissProt ID

P37217 (Mouse), Q07108 (Human)

Gene ID

12515 (Cd69), 969 (CD69)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Chimp (Chimpanzee), Rhesus Mky (Rhesus Monkey), Bab (Baboon)

Available hosts

Hamster, Rabbit, Mouse

Available applications

Functional assay (FN), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD69 antibody

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

General readings

Li J., et al. (2013) Cell. Immunol. 285:23-30.Gonzalez-Amaro R., et al. (2013) Trends Mol. Med. 19(10):625-632.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD69 antibody

Surface staining of Human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57). Mouse IgG1 FN50 Aff - Purified Hu C, F, FN, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Mouse FN50 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD69 antibody

Surface staining of ConA-stimulated splenocytes with  Anti-Mouse CD69 PE .  Appropriate isotype controls were used  (open histogram).  Total viable cells were used for analysis. Hamster H1.2F3 Purified Ms F, IP, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD69 antibody

Predicted band size: 23 kDa . Positive control: Lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD69 antibody

Western blot analysis of extracts of various cell lines, using CD69 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD69 antibody

CD69 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD69 antibody

Western blot analysis of CD69 expression in Jurkat cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD69 antibody

Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57). Mouse IgG1 FN50 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD69 antibody

Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57). Mouse IgG1 FN50 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD69 antibody

Surface staining of human peripheral blood using anti-CD69 antibody (AM03132PP-N) after overnight activation of T cells by anti-CD3 (clone MEM-57). Mouse IgG1 FN50 PerCP Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD69 antibody

Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57). Mouse IgG1 FN50 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Mouse IgG1 SMM FITC Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Mouse IgG1 SMM Purified Hu F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Mouse IgG1 SMM PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD69 antibody

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-CD69 mouse mAb Mouse IgG1 8B6 Ascites Hu E, F, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Mouse IgG3 BL-Ac/p26 Lyophilized Hu C, ICC/IF
1 ml / €300.00
  Acris Antibodies GmbH

CD69 antibody

Western blot (WB) analysis of CD69 antibody (Cat.-No.: AP21168PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Hamster IgG HI.2F3 PE-Cy5 Ms F
100 Tests / €440.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Hamster IgG HI.2F3 FITC Ms F
0.1 mg / €290.00
  Acris Antibodies GmbH

CD69 antibody

CD69 Hamster IgG HI.2F3 Purified Ms C, F, IP
0.2 mg / €390.00
  Acris Antibodies GmbH

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