CD70 antibody

Principal name

CD70 antibody

Alternative names for CD70 antibody

CD27 ligand, CD27L, CD27LG, TNFSF7, Tumor necrosis factor ligand superfamily member 7

SwissProt ID

O55237 (Mouse), P32970 (Human), Q3ZDR4 (Pig)

Gene ID

21948 (Cd70), 970 (CD70)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Por (Porcine), Mky (Monkey), Gorilla

Available hosts

Rat, Mouse, Rabbit

Available applications

Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Functional assay (FN), Neutralisation (NEUT)

Background of CD70 antibody

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

General readings

Welten SP., et al. (2013) J. Virol. 87:6851-6865.Polak ME., et al. (2012) J. Invest. Dermatol. 132:1636-1644.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD70 antibody

Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 (Cat.-No AM05527PU), red in A and Mouse anti Human CD21 antibody, clone LB21, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power. Mouse IgG1 Bu69 Purified Hu C, F
25 µg / €180.00
  Acris Antibodies GmbH

CD70 antibody

Sample (30 ug of whole cell lysate). A: JurKat. 12% SDS PAGE. CD27L (CD70) antibody. TA308292 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD70 antibody

Western blot analysis of extracts from HuvEC, K562, HepG2, Jurkat, HeLa and COLO205 cells, using CD70 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD70 antibody

Anti-CD70 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Hu, Mky
50 µg / €345.00
  OriGene Technologies, Inc.

CD70 antibody

Anti-CD70 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Gorilla, Hu, Mky
50 µg / €345.00
  OriGene Technologies, Inc.

CD70 antibody

Anti-CD70 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Hu
50 µg / €345.00
  OriGene Technologies, Inc.

CD70 antibody

Surface staining of LPS and Anti-Mouse/Rat CD40 Functional Grade Purified stimulated mouse splenocytes with Anti-Mouse CD70 PE .  Autofluorescence is shown via open histogram.  Total viable cells were used for analysis. Rat FR70 Purified Ms F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD70 antibody

Predicted band size: 21 kDa . Positive control: NCI-H460 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD70 antibody

Western blot analysis of extracts of various cell lines, using CD70 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD70 antibody

Western blot analysis of CD70 expression in COLO205 cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD70 antibody

CD70 Mouse IgG1 Bu69 Purified Hu C, F
0.2 mg / €480.00
  Acris Antibodies GmbH

CD70 antibody

CD70 Rat IgG2b FR70 FITC Ms F
0.5 mg / €600.00
  Acris Antibodies GmbH

CD70 antibody

CD70 Mouse IgG3 Ki-24 Purified Hu F
0.1 mg / €210.00
  Acris Antibodies GmbH

CD70 antibody

CD70 Mouse IgG3 Ki-24 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD70 antibody

Western Blot analysis of CD70 expression in transfected 293T cell line (H00000970-T01) by CD70 MaxPab polyclonal antibody.

Lane 1: TNFSF7 transfected lysate(21.23 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD70 antibody

CD70 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CD70 (N-term) antibody

Human ACHN; WB Suggested Anti-CD70 Antibody. . Titration: 1.0 ug/ml. . Positive Control: ACHN Whole Cell. CD70 is supported by BioGPS gene expression data to be expressed in ACHN.; CD70 antibody - N-terminal region (ARP61309_P050) in Human ACHN cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

CD70 antibody

Western blot analysis of CD70 expression in Jurkat (A); NCIH460 (B) whole cell lysates. Rabbit Aff - Purified Hu IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CD70 antibody

Western blot analysis of CD70 expression in Jurkat (A); NCIH460 (B) whole cell lysates. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CD70 antibody

Western blot analysis of CD70 expression in Jurkat (A); NCIH460 (B) whole cell lysates. Rabbit Aff - Purified Hu IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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