CD73 antibody

Principal name

CD73 antibody

Alternative names for CD73 antibody

NT5, NTE, NT5E, 5'-nucleotidase

SwissProt ID

P21588 (Rat), P21589 (Human), Q05927 (Bovin), Q61503 (Mouse)

Gene ID

4907 (NT5E), 23959 (Nt5e)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of CD73 antibody

The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5'-nucleotidase. CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5'-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5'-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.

General readings

Sern P.C, et al., (2006) Purinergic Signal 2(2):351-360
Linda F.T, et al., (2004) J Exp Med 200(11):1395-1405
Laura A, et al., (1997) J Cell Biol 136(2):421-431

  1. Quick-Filters:
  2. Highly Validated (1)
  3. Overnight Delivery (1)

Items 1 to 20 of 37 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD73 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NT5E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809085)(1:150) Mouse IgG1 OTI1G2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD73 antibody

Western blot analysis of extracts from A549 cells and JK cells, using NT5E antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD73 antibody

CD73 (NT5E) Antibody (C-term)(Cat. #TA324900) western blot analysis in MDA-MB-231,WiDr cell line lysates (35ug/lane).This demonstrates the CD73 (NT5E) antibody detected the CD73 (NT5E) protein (arrow). Mouse IgG1 974CT7.5.3 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD73 antibody

Western blot analysis of extracts of various cell lines, using NT5E antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD73 (27-252) antibody

Immunohistochemistry:  AM03116PU-N CD73 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 2B6 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD73 (27-252) antibody

Immunohistochemistry:  AM03116PU-N CD73 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 2B6 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CD73 antibody

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), cholangiocarcinorna (B), lymph node (C) and esophagus (D) tissues using NT5E mouse mAb with DAB staining. Mouse IgG1 1D7 Ascites Hu E, P
0.1 ml / €410.00
  Acris Antibodies GmbH

CD73 antibody

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176. Mouse IgG1 AD2 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD73 antibody

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176. Mouse IgG1 AD2 Purified Hu F
0.1 mg / €210.00
  Acris Antibodies GmbH

CD73 antibody

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176. Mouse IgG1 AD2 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD73 antibody

Flow cytometric detection of human CD73 on Huvec (mAb 4G4, AM26202PU-N). Black, red and blue line represents cells only, isotype control and 4G4 (4 µg/ml, respectively). Mouse IgG1 4G4 Purified Hu C, FN, ICC/IF, IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CD73 (N-term) antibody

Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human kidney tissue stained with CD73 (NT5E) Antibody (N-term) (Cat.-No AP11872PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CD73 (NT5E) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

CD73 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD73 (C-term) antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with NT5E antibody AP22774PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms E, P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

CD73 antibody

Western Blot analysis of NT5E expression in transfected 293T cell line by NT5E monoclonal antibody (M01), clone 4C4-2B5.

Lane 1: NT5E transfected lysate(30.36 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 4C4-2B5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD73 antibody

CD73 Mouse IgG1 4D3-2C10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD73 antibody

Western blot analysis of cell lysate of human mesenchymal stem cell ( 30 µg ) was resolved by SDS - PAGE , transferred to NC membrane and probed with NT5E monoclonal antibody , clone 2B6 ( 1 : 1000 ) ( Cat # MAB1114 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 2B6 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

CD73 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with NT5E polyclonal antibody ( Cat # PAB3845 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CD73 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with NT5E polyclonal antibody ( Cat # PAB3846 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CD73 antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD73 Antibody-FITC labled. Mouse IgG1 FITC Hu F, ICC/IF
50 Assays / €250.00
  Cohesion Biosciences Ltd.

Items 1 to 20 of 37 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn