CD73 antibody

Principal name

CD73 antibody

Alternative names for CD73 antibody

NT5, NTE, NT5E, 5'-nucleotidase

SwissProt ID

P21588 (Rat), P21589 (Human), Q05927 (Bovin), Q61503 (Mouse)

Gene ID

4907 (NT5E), 23959 (Nt5e)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Functional assay (FN), Frozen Sections (C)

Background of CD73 antibody

CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5’-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5’-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo.
In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency.

General readings

Misumi Y., et al. (1990) Eur. J. Biochem. 191:563-569
Hansen K.R., et al. (1995) Gene. 167:307-312

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD73 antibody

  NT5E mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NT5E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809085)(1:150) Mouse IgG1 OTI1G2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD73 (27-252) antibody

Immunohistochemistry:  AM03116PU-N CD73 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 2B6 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD73 (27-252) antibody

Immunohistochemistry:  AM03116PU-N CD73 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 2B6 Purified Hu E, F, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CD73 antibody

  Rabbit polyclonal anti-NT5E antibody  
Western blot analysis of extracts from A549 cells and JK cells, using NT5E antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD73 antibody

  Mouse monoclonal CD73(NT5E) Antibody (C-term)(Ascites)  
CD73 (NT5E) Antibody (C-term)(Cat. #TA324900) western blot analysis in MDA-MB-231,WiDr cell line lysates (35ug/lane).This demonstrates the CD73 (NT5E) antibody detected the CD73 (NT5E) protein (arrow). Mouse IgG1 974CT7.5.3 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD73 antibody

  NT5E Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using NT5E antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD73 antibody

  Rabbit Polyclonal Anti-NT5E Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: A549 cells, Primary antibody: (NT5E Antibody) at dilution 1/850, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD73 antibody

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), cholangiocarcinorna (B), lymph node (C) and esophagus (D) tissues using NT5E mouse mAb with DAB staining. Mouse IgG1 1D7 Ascites Hu E, P
0.1 ml / €410.00
  Acris Antibodies GmbH

CD73 antibody

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176. Mouse IgG1 AD2 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD73 antibody

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176. Mouse IgG1 AD2 Purified Hu F
0.1 mg / €210.00
  Acris Antibodies GmbH

CD73 antibody

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD73 (AD2) Yb176. Mouse IgG1 AD2 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD73 antibody

Flow cytometric detection of human CD73 on Huvec (mAb 4G4, AM26202PU-N). Black, red and blue line represents cells only, isotype control and 4G4 (4 µg/ml, respectively). Mouse IgG1 4G4 Purified Hu C, FN, ICC/IF, IP, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

CD73 (N-term) antibody

Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human kidney tissue stained with CD73 (NT5E) Antibody (N-term) (Cat.-No AP11872PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CD73 (NT5E) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

CD73 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD73 (C-term) antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with NT5E antibody AP22774PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

NT5E - human gene knockout kit via CRISPR

NT5E - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NT5E - human gene knockout kit via CRISPR

NT5E - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NT5E - human gene knockout kit via CRISPR

NT5E - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NT5E - human gene knockout kit via CRISPR

NT5E - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nt5e - mouse gene knockout kit via CRISPR

Nt5e - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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