CD74 antibody

Principal name

CD74 antibody

Alternative names for CD74 antibody

p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain

SwissProt ID

P04233 (Human), P04441 (Mouse), P10247 (Rat)

Gene ID

972 (CD74)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bab (Baboon)

Available hosts

Mouse, Rabbit, Goat

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of CD74 antibody

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing. CLIP or the class II associated invariant chain peptide region of the invariant chain (Ii) has several important functions in the MHC class II antigen processing pathway. In specialized antigen presenting cells (APCs) such as macrophages, B cells and dendritic cells the MHC class II alpha-beta dimers assemble in the endoplasmic reticulum (ER) with a trimer of the nonpolymorphic invariant chain to form a nonameric complex which is transported through the Golgi apparatus to the trans Golgi network. In the trans Golgi network, signals to the invariant chain cytosolic tail target the complex to organelles in the endocytic pathway. Here the peptides derived from antigens, which are usually internalized into the endocytic pathway of antigenpresenting cells, are prevented from binding MHC class II molecules which are at the early stages of their bioynthesis. This prevention is mediated by the membrane proximal domain of Ii which is degraded upon arrival in the endocytic structures prior to peptide loading and these fragments are termed CLIP which consists of amino acids 81 to 104 of Ii.

General readings

Shi GP et al. Role for cathepsin F in invariant chain processing and major histocompatibility complex class II peptide loading by macrophages. J Exp Med 191:1177-86 (2000). PubMed PMID: 10748235
Hammond C et al. The tetraspan protein CD82 is a resident of MHC class II compartments where it associates with HLA-DR, -DM, and -DO molecules. J Immunol 161:3282-91 (1998). PubMed PMID: 9759843
Watts C Capture and processing of exogenous antigens for presentation on MHC molecules. Annu Rev Immunol 15:821-50 (1997). PubMed PMID: 9143708

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD74 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD74 Antibody Cat.-No AM32811PU (Clone LN2). Mouse IgG1 LN2 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD74 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD74 Antibody Cat.-No AM32811PU (Clone LN2). Mouse IgG1 LN2 Purified Bab, Hu, Ms C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD74 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD74 Antibody Cat.-No AM32811PU (Clone LN2). Mouse IgG1 LN2 Purified Bab, Hu, Ms C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD74 antibody

CD74 antibody [N1N2], N-term detects CD74 protein by Western blot analysis. A. 30 ug GL261 whole cell lysate/extract. 12 % SDS-PAGE. CD74 antibody [N1N2], N-term (TA308865) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD74 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507336) Mouse IgG1 OTI1G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD74 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507339) Mouse IgG1 OTI1H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD74 antibody

Formalin Fixed, Formalin-Paraffin Human tonsil stained with CD74 Antibody Cat.-No AM33264PU (Clone SPM523). Note membrane staining. Mouse IgG1 SPM523 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD74 antibody

Formalin Fixed, Formalin-Paraffin Human tonsil stained with CD74 Antibody Cat.-No AM33264PU (Clone SPM523). Note membrane staining. Mouse IgG1 SPM523 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD74 antibody

Formalin Fixed, Formalin-Paraffin Human tonsil stained with CD74 Antibody Cat.-No AM33264PU (Clone SPM523). Note membrane staining. Mouse IgG1 SPM523 Purified Hu, Ms C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD74 antibody

Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF). Mouse IgG1 PIN.1 Purified Hu C, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD74 antibody

Immunohistochemistry staining of Paraffin Tonsil tissue by CD74 antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic, membranous) Mouse IgG1 LN2 Purified Hu P
1 ml / €840.00
  OriGene Technologies, Inc.

CD74 antibody

Western blot analysis of extracts of various cell lines, using CD74 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD74 (alpha+beta) antibody

TA334191 (0.03µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD74 (159-171) antibody

TA334192 (0.01µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD74 antibody

CD74 Mouse IgG1 Bu45 FITC Hu F
0.1 mg / €390.00
  Acris Antibodies GmbH

CD74 antibody

CD74 Mouse IgG1 Bu45 FITC Hu F
25 µg / €190.00
  Acris Antibodies GmbH

CD74 antibody

CD74 Mouse IgG1 Bu45 Purified Hu C, F, FN, ICC/IF, IP
0.2 mg / €430.00
  Acris Antibodies GmbH

CD74 antibody

CD74 Mouse IgG1 Bu45 Purified Hu C, IP
25 µg / €180.00
  Acris Antibodies GmbH

CD74 antibody

Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF). Mouse IgG1 PIN.1 Purified Hu C, ICC/IF, IP, WB
25 µg / €200.00
  Acris Antibodies GmbH

CD74 antibody

Staining of human peripheral blood lymphocytes with mouse anti human CD74 (SM2102P). Mouse IgG1 AT14/19 Purified Hu F, IP
0.2 mg / €440.00
  Acris Antibodies GmbH

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