CD75 / ST6GAL1 antibody

Principal name

CD75 / ST6GAL1 antibody

Alternative names for CD75 / ST6GAL1 antibody

SIAT1, B-Cell marker, Beta-galactoside alpha-2,6-sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1

SwissProt ID

P13721 (Rat), P15907 (Human), Q64685 (Mouse)

Gene ID

6480 (ST6GAL1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD75 / ST6GAL1 antibody

CDw75 (also known as CD75s) is an alpha (2,6)-sialylated neuraminidase-sensitive lymphocyte cell surface differentiation antigen synthesized by the ST6Gal 1 enzyme (Bast, 1992; Kumar, 2012). CDw75 was characterized in 1989 at the 4th Leucocyte Typing Workshop (code number LN1) as an antigen or differentiation marker expressed by the majority of B cells. The LN-1 CDw75 antibody clone is a well-established B lymphocyte marker and detects CDw75 on germinal center B lymphocytes and leukemias/lymphomas and other tissues (Epstein, 1984). The antibody is highly published and widely recognized as a reliable and robust CDw75 antibody for identifying or confirming a B cell phenotype in known lymphoid tissues.
Overall, the analysis of lymphocyte populations in human lymphoid tissues is greatly facilitated by using antibody markers against lymphoid differentiation antigens such as CDw75. The CDw75 antibody may be used in of itself or as part of an antibody panel to localize lymphocyte subsets in the lymph node, spleen, and thymus as well as for immunophenotypic characterization of malignancies.
However, it is important to note that CD molecules like CDw75 are typically not strictly specific for a single hematopoietic lineage and a considerable number are not leukocyte restricted (Moller, 1991). In this regard, CDw75 is also expressed on a large spectrum of nonlymphoid cells (reviewed in Epstein, 1984 & Moller, 1991). The spectrum of CDw75 antibody reactivity includes RBC precursors of bone marrow, epithelium of kidney, breast, prostate, pancreas, and lung, glioblastomas, astrocytomas, colon carcinomas and Reed-Sternberg cells in Hodgkin's lymphoma.
The activity of ST6Gal 1, the enzyme that synthesizes CDw75, may be modified during carcinogenesis and be associated with emergence of the CDw75 antigen. For example, CDw75 was found to be expressed in a subset of colon carcinomas but not in healthy disease-free nor in inflammatory colorectal mucosa (Costa-Noguiera, 2009; Villar-Portela, 2011). These results suggested that the CDw75 antibody may also be a useful antibody marker of tumor progression and malignant potential in certain solid tumors (Costa-Noguiera, 2009; Villar-Portela, 2011).

General readings

Kuhn B., et al. (2013) Acta Crystallogr D Biol Crystallogr. 69:1826-1838.Wu ZL., et al. (2011) Glycobiology. 21:727-733.

Items 1 to 20 of 32 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD75 / ST6GAL1 antibody

Western blot analysis of extracts from RAW264.7 cells, using ST6GAL1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD75 / ST6GAL1 antibody

A typical staining pattern with the B-L5 monoclonal antibody of Raji cell line Mouse IgM B-L5 Azide Free Hu F
0.2 mg / €270.00
  Acris Antibodies GmbH

CD75 / ST6GAL1 antibody

A typical staining pattern with the B-L5 monoclonal antibody of Raji cell line Mouse IgM B-L5 Purified Hu F
2ml (200T) / €250.00
  Acris Antibodies GmbH

CD75 / ST6GAL1 antibody

Surface staining of Human lymphocytes with CDw75 Monoclonal Antibody (LN-1) (red) and isotype control (green).PPI negative population analyzed. Mouse IgM LN-1 Purified Hu F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

CD75 / ST6GAL1 antibody

Western blot (WB) analysis of CD75 antibody (Cat.-No.: AP21150PU-N) in extracts from RAW264.7 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD75 / ST6GAL1 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD75 / ST6GAL1 antibody

Western Blot analysis of ST6GAL1 expression in transfected 293T cell line (H00006480-T01) by ST6GAL1 MaxPab polyclonal antibody.

Lane 1: ST6GAL1 transfected lysate(19.25 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD75 / ST6GAL1 antibody

ST6GAL1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ST6GAL1 expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD75 / ST6GAL1 antibody

Western Blot detection against Immunogen (36.74 KDa) Mouse IgG2a 2E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD75 / ST6GAL1 antibody

CD75 / ST6GAL1 Mouse IgM LN-1 Hu C, P
1 ml / €820.00
  Abnova Taiwan Corp.

CD75 / ST6GAL1 antibody

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using ST6GAL1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

CD75 / ST6GAL1 antibody

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using ST6GAL1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

CD75 / ST6GAL1 antibody

Immunohistochemical analysis of CD75 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CD75 / ST6GAL1 antibody

Immunohistochemical analysis of CD75 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CD75 / ST6GAL1 antibody

Immunohistochemical analysis of CD75 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CD75 / ST6GAL1 antibody

CD75 / ST6GAL1 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

CD75 / ST6GAL1 antibody

CD75 / ST6GAL1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

CD75 / ST6GAL1 antibody

CD75 / ST6GAL1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD75 / ST6GAL1 (transcript variant 2)

CD75 / ST6GAL1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD75 / ST6GAL1 (27-406, His-tag)

CD75 / ST6GAL1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Items 1 to 20 of 32 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn